Antisense Repression of Vacuolar and Cell Wall Invertase in Transgenic Carrot Alters Early Plant Development and Sucrose Partitioning

Abstract Background: SYP71, the plant-specific Qc-SNARE protein, is reported to regulate vesicle trafficking. SYP71 is localized on the ER, endosome, plasma membrane and cell plate, suggesting its multiple functions. Lotus SYP71 is essential for symbiotic nitrogen fixation in nodules. AtSYP71, GmSYP71 and OsSYP71 are implicated in plant resistance to pathogenesis. To date, SYP71 regulatory role on plant development remain unclear.Results: AtSYP71-knockout mutant atsyp71-4 was lethal at early development stage. Early development of AtSYP71-knockdown mutant atsyp71-2 was delayed, and stress response was also affected. Confocal images revealed that protein secretion was blocked in atsyp71-2. Transcriptomic analysis indicated that metabolism, response to environmental stimuli pathways and apoplast components were influenced in atsyp71-2. Moreover, the contents of lignin, cellulose and flavonoids as well as cell wall structures were also altered.Conclusion: Our findings suggested that AtSYP71 is essential for plant development. AtSYP71 probably regulates plant development, metabolism and environmental adaptation by affecting cell wall homeostasis via mediating secretion of materials and regulators required for cell wall biosynthesis and dynamics.

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Induction of a Pea Cell-Wall Invertase Gene by Wounding and Its Localized Expression in Phloem

PLANT PHYSIOLOGY ◽

10.1104/pp.112.3.1111 ◽

1996 ◽

Vol 112 (3) ◽

pp. 1111-1117 ◽

Cited By ~ 45

Author(s):

L. Zhang ◽

N. S. Cohn ◽

J. P. Mitchell

Keyword(s):

Cell Wall ◽

Cell Wall Invertase ◽

Invertase Gene

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Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

Frontiers in Plant Science ◽

10.3389/fpls.2019.00541 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 5

Author(s):

Wei Yan ◽

Xiaoyun Wu ◽

Yanan Li ◽

Guanghua Liu ◽

Zhanfei Cui ◽

...

Keyword(s):

Cell Wall ◽

Cell Wall Invertase ◽

Source To Sink

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Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

Functional Plant Biology ◽

10.1071/fp16436 ◽

2017 ◽

Vol 44 (12) ◽

pp. 1160 ◽

Cited By ~ 1

Author(s):

Zhen-Yuan Ruan ◽

Xiao-Ming Chen ◽

Pu Yang ◽

Bing-Yi Wang

Keyword(s):

Cell Wall ◽

Invertase Activity ◽

Activity Level ◽

Rapid Expansion ◽

Sink Strength ◽

Cell Wall Invertase ◽

Vacuolar Invertase ◽

Growing Period ◽

Rhus Chinensis ◽

Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

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