Three-dimensional imaging with coherent X-rays at nano-scale resolution and beyond

The progress of tomographic coherent diffractive imaging with hard X-rays at the ID10 beamline of the European Synchrotron Radiation Facility is presented. The performance of the instrument is demonstrated by imaging a cluster of Fe2P magnetic nanorods at 59 nm 3D resolution by phasing a diffraction volume measured at 8 keV photon energy. The result obtained shows progress in three-dimensional imaging of non-crystalline samples in air with hard X-rays.

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Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

IUCrJ ◽

10.1107/s205225251501235x ◽

2015 ◽

Vol 2 (5) ◽

pp. 575-583 ◽

Cited By ~ 47

Author(s):

Jose A. Rodriguez ◽

Rui Xu ◽

Chien-Chun Chen ◽

Zhifeng Huang ◽

Huaidong Jiang ◽

...

Keyword(s):

Neospora Caninum ◽

Mass Density ◽

Three Dimensional ◽

Three Dimensions ◽

Natural State ◽

X Rays ◽

Diffractive Imaging ◽

Three Dimensional Imaging ◽

Whole Cells ◽

Dimensional Reconstruction

A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydratedNeospora caninumcell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.

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Three-dimensional imaging of nanoscale materials by using coherent x-rays

10.2172/1011392 ◽

2011 ◽

Author(s):

Jianwei Miao

Keyword(s):

Three Dimensional ◽

Nanoscale Materials ◽

X Rays ◽

Three Dimensional Imaging

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Report on a project on three-dimensional imaging of the biological cell by single-particle X-ray diffraction

Acta Crystallographica Section A Foundations of Crystallography ◽

10.1107/s010876730705550x ◽

2007 ◽

Vol 64 (1) ◽

pp. 33-35 ◽

Cited By ~ 15

Author(s):

D. Sayre

Keyword(s):

Single Particle ◽

High Vacuum ◽

Three Dimensional ◽

X Rays ◽

X Ray Diffraction ◽

Acta Cryst ◽

Three Dimensional Imaging ◽

X Ray ◽

X Ray Crystallography ◽

A Cell

Single-particle X-ray diffraction is an extension of X-ray crystallography which allows the specimen to be any small solid-state bounded object; in Shapiroet al.[Proc. Natl Acad. Sci. USA(2005),102, 15343–15346] and Thibaultet al.[Acta Cryst.(2006), A62, 248–261], the reader can find descriptions of a recent StonyBrook/Berkeley/Cornell two-dimensional imaging of a yeast cell by this technique. Our present work is aimed at extending the technique to the three-dimensional imaging of a cell. However, the usual method of doing that, namely rotating the specimen into many orientations in the X-ray beam, has not as yet given sufficiently good three-dimensional diffraction data to allow the work to go forward, the largest problem being the difficulty of preventing unwanted levels of change in the specimen through the extended exposure to a hostile environment of X-rays and, in some cases, high vacuum and/or extreme cold. The present paper discusses possible methods of dealing with this problem.

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Ultrafast Three-Dimensional Imaging of Lattice Dynamics in Individual Gold Nanocrystals

Science ◽

10.1126/science.1236034 ◽

2013 ◽

Vol 341 (6141) ◽

pp. 56-59 ◽

Cited By ~ 186

Author(s):

J. N. Clark ◽

L. Beitra ◽

G. Xiong ◽

A. Higginbotham ◽

D. M. Fritz ◽

...

Keyword(s):

Time Scale ◽

Three Dimensional ◽

Laser Pulses ◽

X Rays ◽

Three Dimensional Imaging ◽

Pump Probe ◽

Gold Nanocrystals ◽

Dynamics Simulations ◽

Pump Probe Experiment ◽

Gold Nanocrystal

Key insights into the behavior of materials can be gained by observing their structure as they undergo lattice distortion. Laser pulses on the femtosecond time scale can be used to induce disorder in a “pump-probe” experiment with the ensuing transients being probed stroboscopically with femtosecond pulses of visible light, x-rays, or electrons. Here we report three-dimensional imaging of the generation and subsequent evolution of coherent acoustic phonons on the picosecond time scale within a single gold nanocrystal by means of an x-ray free-electron laser, providing insights into the physics of this phenomenon. Our results allow comparison and confirmation of predictive models based on continuum elasticity theory and molecular dynamics simulations.

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Three-dimensional imaging of acoustic phonons using an X-ray laser

Acta Crystallographica Section A Foundations and Advances ◽

10.1107/s2053273314097113 ◽

2014 ◽

Vol 70 (a1) ◽

pp. C288-C288

Author(s):

Jesse Clark ◽

Ian Robinson

Keyword(s):

Time Scale ◽

Three Dimensional ◽

Laser Pulses ◽

X Rays ◽

Acoustic Phonons ◽

Three Dimensional Imaging ◽

Gold Nanocrystals ◽

X Ray ◽

Dynamics Simulations ◽

Pump Probe Experiment

"Key insights into the behaviour of materials can be gained by observing their structure when they undergo lattice distortion. Laser pulses on the femtosecond time scale can be used to induce disorder in a ``pump-probe"" experiment with the ensuing transients being probed stroboscopically using femtosecond pulses of visible light, X-rays or electrons (Fig. 1 A). Here we report two- and three-dimensional imaging of the generation and subsequent evolution of coherent acoustic phonons on the picosecond time scale within single gold nanocrystals using an X-ray free electron laser (Fig. 1 B). Our results allow comparisons between experiment and predictive models based on continuum elasticity theory and molecular dynamics simulations (Fig. 1 B). *Full author list - J.N. Clark L. Beitra, G. Xiong, A. Higginbotham, D. M. Fritz, H. T. Lemke, D. Zhu, M. Cholle, G. J. Williams, M. Messerschmidt, B. Abbey, R. J. Harder, A. M. Korsunsky, J. S. Wark, D. Reis, I. K. Robinson."

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Measurements in 3D images

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100086349 ◽

1991 ◽

Vol 49 ◽

pp. 408-409

Author(s):

John C. Russ

Keyword(s):

Three Dimensional ◽

Image Plane ◽

X Rays ◽

Traditional Use ◽

3D Images ◽

Confocal Scanning Laser Microscope ◽

Hard Materials ◽

Depth Direction ◽

Confocal Scanning ◽

Confocal Scanning Laser

Three-dimensional (3D) images consisting of arrays of voxels can now be routinely obtained from several different types of microscopes. These include both the transmission and emission modes of the confocal scanning laser microscope (but not its most common reflection mode), the secondary ion mass spectrometer, and computed tomography using electrons, X-rays or other signals. Compared to the traditional use of serial sectioning (which includes sequential polishing of hard materials), these newer techniques eliminate difficulties of alignment of slices, and maintain uniform resolution in the depth direction. However, the resolution in the z-direction may be different from that within each image plane, which makes the voxels non-cubic and creates some difficulties for subsequent analysis.

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