Crystallization of an intact GST-estrogen receptor hormone binding domain fusion protein
1998 ◽
Vol 54
(3)
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pp. 423-426
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Keyword(s):
Crystals of an intact GST–estrogen receptor hormone binding domain fusion protein have been grown from solutions of MPD. The crystals grew as clusters of thin plates and needles of maximum dimensions 100 × 20 × 1 µm but were unsuitable for X-ray diffraction analysis. However, examination by electron microscopy shows an ordered lattice in which the protein molecules are clearly visible. Image analysis of electron micrographs of the protein crystals revealed electron stain-excluding density which showed a two-domain trimeric structure in projection, with each molecule of dimensions 12.0 × 5.0 nm diameter. The use of GST-fusion proteins in crystallization are discussed.
1991 ◽
Vol 266
(17)
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pp. 10880-10887
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1996 ◽
Vol 58
(1)
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pp. 21-30
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Keyword(s):
1993 ◽
Vol 7
(11)
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pp. 1408-1417
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1996 ◽
Vol 271
(33)
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pp. 20053-20059
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Keyword(s):
2003 ◽
Vol 46
(14)
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pp. 2865-2876
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Keyword(s):
1992 ◽
Vol 267
(14)
◽
pp. 9868-9873
Keyword(s):
1994 ◽
Vol 48
(5-6)
◽
pp. 447-452
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Keyword(s):