Background:
Based on gold nanoparticles (AuNPs) and thiol graphene (TGR) nanocomposite modified carbon
ionic liquid electrode, an electrochemical DNA biosensor was prepared to detect specific nuc gene of Staphylococcus
aureus, which was further used to the analysis of PCR amplification samples from unfrozen pork.
Objective:
The construction of DNA biosensor with AuNPs and TGR could be used as a new sensing platform to detect
specific ssDNA sequence in real biological samples.
Method:
Electrochemical behaviors of working electrodes were studied by cyclic voltammetry and electrochemical
impedance spectroscopy with electrochemical performances of this biosensor investigated by differential pulse
voltammetry.
Results:
This gene sensor could detect the specific nuc gene of Staphylococcus aureus in the linear concentration range
from 1.0×10-15 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 4.5×10-16 mol L-1 (3σ), and it was applied to the
detection of PCR amplification sample of Staphylococcus aureus with satisfactory results.
Conclusion:
This gene biosensor showed high sensitivity and good selectivity, wide detection range and low detection limit,
which demonstrated an effective tool to detect specific nuc gene sequences of Staphylococcus aureus.
Label-Free Electrochemical DNA Biosensor for the Detection of Mycobacterium Tuberculosis Using Gold Electrode Modified by Self-Assembled Monolayer of Thiol
