Investigation of Electrochemical DNA Biosensor for Detection of Specific nuc Gene of Staphylococcus aureus Based on Gold Nanoparticles and Thiol Graphene Nanocomposite

2020 ◽  
Vol 16 ◽  
Author(s):  
Lijun Yan ◽  
Bo Shao ◽  
Xiaoping Zhang ◽  
Yanyan Niu ◽  
Wendi Dang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Electrochemical Impedance ◽  
Pcr Amplification ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Electrochemical Performances ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: Based on gold nanoparticles (AuNPs) and thiol graphene (TGR) nanocomposite modified carbon ionic liquid electrode, an electrochemical DNA biosensor was prepared to detect specific nuc gene of Staphylococcus aureus, which was further used to the analysis of PCR amplification samples from unfrozen pork. Objective: The construction of DNA biosensor with AuNPs and TGR could be used as a new sensing platform to detect specific ssDNA sequence in real biological samples. Method: Electrochemical behaviors of working electrodes were studied by cyclic voltammetry and electrochemical impedance spectroscopy with electrochemical performances of this biosensor investigated by differential pulse voltammetry. Results: This gene sensor could detect the specific nuc gene of Staphylococcus aureus in the linear concentration range from 1.0×10-15 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 4.5×10-16 mol L-1 (3σ), and it was applied to the detection of PCR amplification sample of Staphylococcus aureus with satisfactory results. Conclusion: This gene biosensor showed high sensitivity and good selectivity, wide detection range and low detection limit, which demonstrated an effective tool to detect specific nuc gene sequences of Staphylococcus aureus.

Electrochemical DNA biosensor based on platinum-gold bimetal decorated graphene modified electrode for the detection of Vibrio Parahaemolyticus specific tlh gene sequence

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijun Yan ◽  
Fan Shi ◽  
Jingyao Zhang ◽  
Yanyan Niu ◽  
Lifang Huang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Modified Electrode ◽  
Vibrio Parahaemolyticus ◽  
Gene Sequence ◽  
Modified Electrodes ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: By using bimetal nanocomposite modified electrode, the electrochemical DNA biosensor showed the advantages of high sensitivity, low cost, rapid response and convenient operation, which was applied for disease diagnosis, food safety, and biological monitoring. Objective: A nanocomposite consisting of platinum (Pt)-gold (Au) bimetal and two-dimensional graphene (GR) was synthesized by hydrothermal method, which was modified on the surface of carbon ionic liquid electrode and further used for the immobilization of probe ssDNA related to Vibrio Parahaemolyticus tlh gene to construct an electrochemical DNA sensor. Method: Potassium ferricyanide was selected as electrochemical indicator, cyclic voltammetry was used to study the electrochemical behaviours of different modified electrodes and differential pulse voltammetry was employed to test the analytical performance of this biosensor for the detection of target gene sequence. Results: This electrochemical DNA biosensor could detect the Vibrio Parahaemolyticus tlh gene sequence as the linear concentration in the range from 1.0×10-13 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 2.91×10-14 mol L-1 (3σ). Conclusion: This proposed electrochemical DNA biosensor could be used to identify the special gene sequence with good selectivity, low detection limit and wide detection range.

Reduced graphene oxide nanoribbon immobilized gold nanoparticle based electrochemical DNA biosensor for the detection of Mycobacterium tuberculosis

2018 ◽  
Vol 6 (31) ◽  
pp. 5181-5187 ◽  
Author(s):  
Navin Kumar Mogha ◽  
Vikrant Sahu ◽  
Raj Kishore Sharma ◽  
Dhanraj T. Masram
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Graphene Oxide ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
High Specificity ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Reduced Graphene ◽  
Very High

A DNA based biosensor is reported with very high specificity to Mycobacterium tuberculosis and a detection limit much superior to those already reported in the literature.

Colorimetric Detection of Trace Arsenic(III) in Aqueous Solution Using Arsenic Aptamer and Gold Nanoparticles

2014 ◽  
Vol 67 (5) ◽  
pp. 813 ◽  
Author(s):  
Minglei Yu
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Colour Change ◽  
Colorimetric Sensing ◽  
Detection Range ◽  
Colorimetric Indicator ◽  
Blue Solution

In this study, trace arsenic(iii) (AsIII) in aqueous solution was detected by applying a classical aptamer-based gold nanoparticles colorimetric sensing strategy. An arsenic aptamer was used as a sensing probe and gold nanoparticles as a colorimetric indicator. In the absence of AsIII, the gold nanoparticles were stabilised by the arsenic aptamer and remained dispersed at high NaCl concentrations, displaying a red solution. Contrarily, in the presence of AsIII, the gold nanoparticles were prone to aggregation, owing to the formation of aptamer–AsIII complex between the arsenic aptamer and AsIII, and thus exhibited a blue solution. By monitoring the colour change, a simple and fast colorimetric assay for AsIII was established with a detection range of 1.26–200 ppb and a detection limit of 1.26 ppb. Because this colorimetric assay only involves common reagents and can be assessed visually, it holds great potential for arsenic(iii) monitoring in environment-related and other applications.

scholarly journals Application of Gold Nanoparticles for Electrochemical DNA Biosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ahmed Mishaal Mohammed ◽  
Ruslinda A. Rahim ◽  
Ibraheem Jaleel Ibraheem ◽  
Foo Kai Loong ◽  
Hasrul Hisham ◽  
...  
Keyword(s):  
Thin Films ◽  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Base Line ◽  
Frequency Range ◽  
Dna Immobilization ◽  
Electrochemical Dna Biosensor ◽  
Chemically Modified

An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2thin films. The SiO2thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2thin films using APTES and GNPs were successfully developed for DNA detection.

scholarly journals An electrochemical DNA biosensor for trace amounts of mercury ion quantification

2016 ◽  
Vol 14 (5) ◽  
pp. 808-815 ◽  
Author(s):  
Ferdaous Maâtouk ◽  
Mouna Maâtouk ◽  
Karima Bekir ◽  
Houcine Barhoumi ◽  
Abderrazak Maaref ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Mercury Ion ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Dna Oligonucleotides ◽  
Wastewater Samples ◽  
Eis Measurements

In this work we report the development of an electrochemical DNA biosensor with high sensitivity for mercury ion detection. A new matrix based on gold nanoparticles (AuNPs)-glutathione (GSH)/cysteine was investigated. The interaction between DNA oligonucleotides and Hg2+ ions followed by the formation of Thymine–Hg2+–Thymine (T–Hg2+–T) structures was quantified using different electrochemical methods. It has been shown that the electrochemical impedance spectroscopy (EIS) measurements and the differential pulse voltammetry (DPV) confirmed the specific interaction between the oligonucleotide receptor layer and the Hg2+ ions. Besides, the developed sensor exhibited high sensitivity towards mercury among some examined metal ions such as Pb2+, Cu2+ and Cd2+. As a result, a high electrochemical response and low detection limit of 50 pM were estimated in the case of Hg2+ ions. The developed DNA biosensor was applied successfully to the determination of Hg2+ions in wastewater samples.

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