This article introduces a measurement system based on LabVIEW used for optical interference fringe on micro-fluidic chips. This system mainly uses cameras to capture real-time images of wedge interference fringe on micro-fluidic chips, then the collected images will be binarized by LabVIEW. The processed images will be divided by zone , determine the flatness and gap thickness of the micro-fluidic chips by interference fringes with different directions of deflection and numbers. Finally, feedback from measured data will be used to adjust the flatness and gap thickness of micro-fluidic chips in order to meet the requirement of tests.