scholarly journals Graph-based image decoding for multiplexed in situ RNA detection

2021 ◽  
Author(s):  
Gabriele Partel ◽  
Carolina Wahlby
Keyword(s):  
Rna Detection ◽  
Image Decoding

scholarly journals Small RNA Detection by in Situ Hybridization Methods

2015 ◽  
Vol 16 (12) ◽  
pp. 13259-13286 ◽  
Author(s):  
Martyna Urbanek ◽  
Anna Nawrocka ◽  
Wlodzimierz Krzyzosiak
Keyword(s):  
In Situ Hybridization ◽  
Small Rna ◽  
Rna Detection

3′-End fluorochromized and haptenized oligonucleotides as in situ hybridization probes for multiple, simultaneous RNA detection

1991 ◽  
Vol 194 (2) ◽  
pp. 310-315 ◽  
Author(s):  
R.W. Dirks ◽  
R.P.M. Van Gijlswijk ◽  
M.A. Vooijs ◽  
A.B. Smit ◽  
J. Bogerd ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rna Detection ◽  
Hybridization Probes

scholarly journals Optimization of Nuclear Transcript Detection by FISH and Combination with Fluorescence Immunocytochemical Detection of Transcription Factors

1997 ◽  
Vol 45 (12) ◽  
pp. 1585-1592 ◽  
Author(s):  
Caroline Jolly ◽  
Fabien Mongelard ◽  
Michel Robert-Nicoud ◽  
Claire Vourc'h
Keyword(s):  
Heat Shock ◽  
High Efficiency ◽  
Simultaneous Detection ◽  
Nuclear Accumulation ◽  
Rna Detection ◽  
Immunocytochemical Detection ◽  
Transcription Factor 1 ◽  
Transcript Detection ◽  
Nuclear Texture

Detection of specific nuclear transcripts by fluorescence in situ hybridization (FISH) has constituted a major breakthrough in the study of the organization of transcription in the cell nucleus. Using the model of heat shock genes, we present an optimized procedure for nuclear transcripts that provides high efficiency for RNA detection and good preservation of cell morphology and nuclear texture. Using this procedure, we designed an original high-efficiency methodology combining FISH and fluorescence immunocytochemistry (FICC), which is used here for the simultaneous detection of heat-shock protein (hsp) nuclear transcripts and the specific heat-shock transcription factor 1 (HSF1). We show that the nuclear accumulation sites of HSF1 in heat-shocked cells do not correspond to the sites of transcription of the hsp70 gene.

scholarly journals RNA detection in situ with FISH-STICs

RNA ◽  
2013 ◽  
Vol 20 (2) ◽  
pp. 260-266 ◽  
Author(s):  
J. R. Sinnamon ◽  
K. Czaplinski
Keyword(s):  
Rna Detection
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