Stereo imaging is not new to electron microscopy. Von Ardenne, who first published transmission pairs nearly forty hears ago, himself refers to a patent application by Ruska in 1934. In the early days of the electron microscope von Ardenne employed a pair of magnetic lenses to view untilted specimens but soon opted for the now standard technique of tilting the specimen with respect to the beam.In the shadow electron microscope stereo images can, of course, be obtained by tilting the specimen between micrographs. This obvious method suffers from the disadvantage that the magnification is very sensitive to small changes in specimen height which accompany tilting in the less sophisticated stages and it is also time consuming. A more convenient method is provided by horizontally displacing the specimen between micrographs. The specimen is not tilted and the technique is both simple and rapid, stereo pairs being obtained in less than thirty seconds.
Sub-pixel matching method for low-resolution thermal stereo images