HV-CMOS single-chip electronics platform for lab-on-chip DNA analysis

2016 ◽  
Author(s):  
David L. Sloan ◽  
Benjamin Martin ◽  
Gordon Hall ◽  
Andrew Hakman ◽  
Philip Marshall ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Single Chip ◽  
Lab On Chip ◽  
On Chip

scholarly journals Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 576 ◽  
Author(s):  
Edgar Jiménez-Díaz ◽  
Mariel Cano-Jorge ◽  
Diego Zamarrón-Hernández ◽  
Lucia Cabriales ◽  
Francisco Páez-Larios ◽  
...  
Keyword(s):  
Soft Lithography ◽  
Low Cost ◽  
Cost Effective ◽  
Microfluidic Chips ◽  
Single Chip ◽  
Lab On Chip ◽  
Molding Method ◽  
On Chip ◽  
Selective Integration ◽  
Important Progress

Microfluidics has become a very promising technology in recent years, due to its great potential to revolutionize life-science solutions. Generic microfabrication processes have been progressively made available to academic laboratories thanks to cost-effective soft-lithography techniques and enabled important progress in applications like lab-on-chip platforms using rapid- prototyping. However, micron-sized features are required in most designs, especially in biomimetic cell culture platforms, imposing elevated costs of production associated with lithography and limiting the use of such devices. In most cases, however, only a small portion of the structures require high-resolution and cost may be decreased. In this work, we present a replica-molding method separating the fabrication steps of low (macro) and high (micro) resolutions and then merging the two scales in a single chip. The method consists of fabricating the largest possible area in inexpensive macromolds using simple techniques such as plastics micromilling, laser microfabrication, or even by shrinking printed polystyrene sheets. The microfeatures were made on a separated mold or onto existing macromolds using photolithography or 2-photon lithography. By limiting the expensive area to the essential, the time and cost of fabrication can be reduced. Polydimethylsiloxane (PDMS) microfluidic chips were successfully fabricated from the constructed molds and tested to validate our micro–macro method.

scholarly journals Fast Track DNA Analysis Suite for human identification

2013 ◽  
Author(s):  
Peter T Docker ◽  
Joanna Baker ◽  
Steve Haswell
Keyword(s):  
Microfluidic Chip ◽  
Fast Track ◽  
Dna Analysis ◽  
Human Identification ◽  
Rapid Analysis ◽  
Human Interaction ◽  
Lab On Chip ◽  
Human Dna ◽  
On Chip ◽  
Proof Of Principle

This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.

scholarly journals Fast Track DNA Analysis Suite for human identification

2013 ◽  
Author(s):  
Peter T Docker ◽  
Joanna Baker ◽  
Steve Haswell
Keyword(s):  
Microfluidic Chip ◽  
Fast Track ◽  
Dna Analysis ◽  
Human Identification ◽  
Rapid Analysis ◽  
Human Interaction ◽  
Lab On Chip ◽  
Human Dna ◽  
On Chip ◽  
Proof Of Principle

This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.

scholarly journals Integration of Biosensors and Associated Electronics on Lab-on-Chip Devices

1970 ◽  
Vol 110 (4) ◽  
pp. 61-66
Author(s):  
A. T. Giannitsis ◽  
T. Parve ◽  
M. Min
Keyword(s):  
Environmental Monitoring ◽  
Point Of Care ◽  
Single Chip ◽  
Sensing Element ◽  
Lab On Chip ◽  
Liquid Samples ◽  
On Chip ◽  
Miniaturized Devices

Lab-on-chip devices comprise a class of bioelectronic miniaturized devices that incorporate microfluidic and biosensing apparatuses on a single chip. They are dedicated for analyzing and processing biochemical liquid samples, which may consist of enzymes, proteins, nucleotides, or even cells and viruses. Furthermore, lab-on-chips may enhance synthesis of biochemical products. The importance of lab-on-chip devices lies on their potentiality of advancing the development of environmental monitoring sensors and also point-of-care analyzers in medicine. This article presents the usual microfabrication methods for manufacturing lab-on-chip devices, with emphasis on the integration of the biosensor, the biocompatibility of the sensing element of the biosensor, and the essential electronics. Three major types of biosensors are analyzed: optical, impedimetric and electrochemical ones. Ill. 7, bibl. 28 (in English; abstracts in English and Lithuanian).http://dx.doi.org/10.5755/j01.eee.110.4.288

scholarly journals Design and Fabrication of Multichannel PDMS Microfluidic

2021 ◽  
Vol 2129 (1) ◽  
pp. 012048
Author(s):  
M N Afnan Uda ◽  
U Hashim ◽  
M N A Uda ◽  
N A Parmin ◽  
V Thivina
Keyword(s):  
Contact Area ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Main Tool ◽  
Morphological Properties ◽  
Single Chip ◽  
Lab On Chip ◽  
Micro Channels ◽  
On Chip ◽  
Disease Analysis

Abstract Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC’s. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.

Numerical Analyses of Microchannels Filling in a Lab-on-Chip Device

2006 ◽  
Author(s):  
Marco Rasponi ◽  
Monica Soncini ◽  
Franco Maria Montevecchi ◽  
Alberto Redaelli
Keyword(s):  
Numerical Model ◽  
Dna Analysis ◽  
Fluid Dynamic ◽  
Contact Angles ◽  
Surface Forces ◽  
Lab On Chip ◽  
Viscous Forces ◽  
Filling Time ◽  
Solid Substrates ◽  
On Chip

A prototype of a Lab-on-Chip (LoC) device manufactured by ST Microelectronics Inc., which is intended to be a diagnostic platform for DNA analysis, has been analyzed. In particular, the dynamics of the filling process was evaluated by means of a 3-D numerical model. Measurements of wettability were also conducted by evaluating the surface tension of the examined liquids and their contact angles on the solid substrates. Two different filling conditions were simulated: pure capillarity and a pressure of 1.5 kPa applied to the inlet. Results in terms of filling time, fluids velocities and percentage of air entrapped in the channels were analyzed. The numerical model revealed the presence of 3.4% of air in the channels (localized in the corner regions), when the pressure of 1.5 kPa was applied. In case of zero pressure, the top corners of the central channel got completely wetted, thus reducing the amount of air to 2.7%. The results showed that capillary forces are dominant during the filling of channels with dimensions smaller than 200 μm. General parameters used to have an insight into the kind of forces leading a fluid-dynamic process are the Reynolds (Re) and Capillary (Ca) numbers, ratios between inertial and viscous forces, and viscous and surface forces, respectively. The computed maximum values in our simulations were Re = 60 and Ca = 0.018, showing the predominance of surface forces on both viscous and, indirectly, inertial ones.

A multilevel Lab on chip platform for DNA analysis

2010 ◽  
Vol 13 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Simone Luigi Marasso ◽  
Eros Giuri ◽  
Giancarlo Canavese ◽  
Riccardo Castagna ◽  
Marzia Quaglio ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Lab On Chip ◽  
On Chip
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