In this article, by utilizing the predefined-time stability theory, the predefined-time output tracking control problem for perturbed uncertain nonlinear systems with pure-feedback structure is addressed. The nonaffine structure of the original system is simplified as an affine form via the property of the mean value theorem. Furthermore, the design difficulty from the uncertain nonlinear function is overcome by the excellent approximation performance of RBF neural networks (NNs). An adaptive predefined-time controller is designed by introducing the finite-time differentiator which is used to decrease the computational complexity problem appeared in the traditional backstepping control. It is proved that the proposed control method guarantees all signals in the closed-loop system remain bound and the tracking error converges to zero within the predefined time. Based on the controller designed in this paper, the expected results can be obtained in predefined time, which can be illustrated by the simulation results.