scholarly journals Focal Surface Detection of High Numerical Aperture Objective Lens Based on Differential Astigmatic Method

2021 ◽  
Vol 13 (4) ◽  
pp. 1-8
Author(s):  
Jia-Lin Du ◽  
Wei Yan ◽  
Li-Wei Liu ◽  
Fan-Xing Li ◽  
Fu-Ping Peng ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Objective Lens ◽  
High Numerical Aperture ◽  
Surface Detection ◽  
Focal Surface

Calibration of polarization effect of a high-numerical-aperture objective lens with Mueller matrix polarimetry

2018 ◽  
Vol 30 (2) ◽  
pp. 025201
Author(s):  
Chao Chen ◽  
Xiuguo Chen ◽  
Honggang Gu ◽  
Hao Jiang ◽  
Chuanwei Zhang ◽  
...  
Keyword(s):  
Polarization Effect ◽  
Numerical Aperture ◽  
Mueller Matrix ◽  
Objective Lens ◽  
High Numerical Aperture

Evaluation of Anisotropic Biaxial Stress by Raman Spectroscopy with a High Numerical Aperture Immersion Objective Lens

2010 ◽  
Vol 49 (4) ◽  
pp. 04DA21 ◽  
Author(s):  
Daisuke Kosemura ◽  
Munehisa Takei ◽  
Kohki Nagata ◽  
Hiroaki Akamatsu ◽  
Ryosuke Shimidzu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Numerical Aperture ◽  
Biaxial Stress ◽  
Objective Lens ◽  
High Numerical Aperture

Signal Simulation of 25 Gbytes Read-Only Optical Disk System using a High Numerical Aperture Objective Lens

2001 ◽  
Vol 40 (Part 1, No. 3B) ◽  
pp. 1536-1541 ◽  
Author(s):  
Takuma Yanagisawa ◽  
Takayuki Nomoto ◽  
Seiichi Ohsawa ◽  
Fumihiko Yokogawa
Keyword(s):  
Numerical Aperture ◽  
Optical Disk ◽  
Objective Lens ◽  
Disk System ◽  
High Numerical Aperture ◽  
Signal Simulation

Optical Disk System Using a High-Numerical Aperture Single Objective Lens and a Blue LD

2000 ◽  
Vol 39 (Part 1, No. 2B) ◽  
pp. 978-979 ◽  
Author(s):  
Makoto Itonaga ◽  
Shuichiro Chaen ◽  
Eiji Nakano ◽  
Hideki Nakamura ◽  
Fumihiko Ito ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Optical Disk ◽  
Objective Lens ◽  
Disk System ◽  
High Numerical Aperture ◽  
Single Objective

scholarly journals High-numerical-aperture cryogenic light microscopy for increased precision of superresolution reconstructions

2017 ◽  
Vol 114 (15) ◽  
pp. 3832-3836 ◽  
Author(s):  
Marc Nahmani ◽  
Conor Lanahan ◽  
David DeRosier ◽  
Gina G. Turrigiano
Keyword(s):  
Single Molecule ◽  
Room Temperature ◽  
Fluorescent Protein ◽  
Numerical Aperture ◽  
Fluorescent Imaging ◽  
Objective Lens ◽  
High Numerical Aperture ◽  
Superresolution Microscopy ◽  
Superresolution Imaging ◽  
Photoactivated Localization Microscopy

Superresolution microscopy has fundamentally altered our ability to resolve subcellular proteins, but improving on these techniques to study dense structures composed of single-molecule-sized elements has been a challenge. One possible approach to enhance superresolution precision is to use cryogenic fluorescent imaging, reported to reduce fluorescent protein bleaching rates, thereby increasing the precision of superresolution imaging. Here, we describe an approach to cryogenic photoactivated localization microscopy (cPALM) that permits the use of a room-temperature high-numerical-aperture objective lens to image frozen samples in their native state. We find that cPALM increases photon yields and show that this approach can be used to enhance the effective resolution of two photoactivatable/switchable fluorophore-labeled structures in the same frozen sample. This higher resolution, two-color extension of the cPALM technique will expand the accessibility of this approach to a range of laboratories interested in more precise reconstructions of complex subcellular targets.

Using high numerical aperture objective lens in micro-reflectance difference spectrometer

2017 ◽  
Vol 421 ◽  
pp. 535-541 ◽  
Author(s):  
Wanfu Shen ◽  
Chunguang Hu ◽  
Shuai Li ◽  
Xiaotang Hu
Keyword(s):  
Numerical Aperture ◽  
Objective Lens ◽  
High Numerical Aperture
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