Nanoparticle Detection with Portable and Low-Cost Lensless Holographic Microscopy Platform

2020 ◽  
Author(s):  
Kerem Delikoyun ◽  
Seren Kecili ◽  
H. Cumhur Tekin
Keyword(s):  
Low Cost ◽  
Nanoparticle Detection ◽  
Holographic Microscopy

scholarly journals Single-Element Reflective Digital Holographic Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
José Ángel Picazo-Bueno ◽  
Javier García ◽  
Vicente Micó
Keyword(s):  
Low Cost ◽  
Digital Holographic Microscopy ◽  
Single Element ◽  
Phase Imaging ◽  
Single Shot ◽  
One Dimensional ◽  
Common Path ◽  
Microscopy Technique ◽  
Digital Holograms ◽  
Holographic Microscopy

Digital holographic microscopy (DHM) is a well-known microscopy technique using an interferometric architecture for quantitative phase imaging (QPI) and it has been already implemented utilizing a large number of interferometers. Among them, single-element interferometers are of particular interest due to its simplicity, stability, and low cost. Here, we present an extremely simple common-path interferometric layout based on the use of a single one-dimensional diffraction grating for both illuminating the sample in reflection and generating the digital holograms. The technique, named single-element reflective digital holographic microscopy (SER-DHM), enables QPI and topography analysis of reflective/opaque objects using a single-shot operation principle. SER-DHM is experimentally validated involving different reflective samples.

scholarly journals A Systematic Review of Food Allergy: Nanobiosensor and Food Allergen Detection

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 194
Author(s):  
Adriano Aquino ◽  
Carlos Adam Conte-Junior
Keyword(s):  
Systematic Review ◽  
Low Cost ◽  
Meta Analysis ◽  
Graphene Quantum Dots ◽  
Protein Detection ◽  
Food Samples ◽  
Accidental Exposure ◽  
Nanoparticle Detection ◽  
Allergen Detection ◽  
Allergenic Proteins

Several individuals will experience accidental exposure to an allergen. In this sense, the industry has invested in the processes of removing allergenic compounds in food. However, accidental exposure to allergenic proteins can result from allergenic substances not specified on labels. Analysis of allergenic foods is involved in methods based on immunological, genetic, and mass spectrometry. The traditional methods have some limitations, such as high cost. In recent years, biosensor and nanoparticles combined have emerged as sensitive, selective, low-cost, and time-consuming techniques that can replace classic techniques. Nevertheless, each nanomaterial has shown a different potential to specific allergens or classes. This review used Preferred Reporting Items for Systematic Reviews and the Meta-Analysis guidelines (PRISMA) to approach these issues. A total of 104 articles were retrieved from a standardized search on three databases (PubMed, Scopus and Web of Science). The systematic review article is organized by the category of allergen detection and nanoparticle detection. This review addresses the relevant biosensors and nanoparticles as gold, carbon, graphene, quantum dots to allergen protein detection. Among the selected articles it was possible to notice a greater potential application on the allergic proteins Ah, in peanuts and gold nanoparticle-base as a biosensor. We envision that in our review, the association between biosensor and nanoparticles has shown promise in the analysis of allergenic proteins present in different food samples.

scholarly journals High accuracy label-free classification of kinetic cell states from holographic cytometry

2017 ◽  
Author(s):  
Miroslav Hejna ◽  
Aparna Jorapur ◽  
Jun S. Song ◽  
Robert L. Judson
Keyword(s):  
Low Cost ◽  
Cancer Therapeutics ◽  
High Accuracy ◽  
Digital Holographic Microscopy ◽  
Adherent Cell ◽  
Label Free ◽  
Multiple Behaviors ◽  
Holographic Microscopy ◽  
Free Classification

AbstractDigital holographic microscopy permits live and label-free visualization of adherent cells. Here we report the application of this approach for high accuracy kinetic quantitative cytometry. We identify twenty-six label-free optical and morphological features that are biologically independent. When used as a basis for machine learning, these features allow blind single cell classification with up to 95% accuracy. We present methods to control for inherent holographic noise, thereby establishing a set of reliable quantitative features. Together, these contributions permit continuous digital holographic cytometry for three or more days. Applying our approach to human melanoma cells treated with a panel of cancer therapeutics, we can track the response of each cell, simultaneously classifying multiple behaviors such as cell cycle length, motility, apoptosis, senescence, and heterogeneity of response to each therapeutic. Importantly, we demonstrate relationships between these phenotypes over time. This work thus provides an experimental and computational roadmap for low cost live-cell imaging and kinetic classification of heterogeneous adherent cell populations.

scholarly journals Development of a Low Cost Digital Holographic Microscopy System

2016 ◽  
Vol 44 (7) ◽  
pp. 454
Author(s):  
Marie SANO ◽  
Junichi ITOI ◽  
Tomoyoshi SHIMOBABA ◽  
Shin ABIRU ◽  
Yutaka ENDO ◽  
...  
Keyword(s):  
Low Cost ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

scholarly journals Single-Entity Detection With TEM-Fabricated Nanopores

2021 ◽  
Vol 9 ◽  
Author(s):  
Hongcheng Yang ◽  
Muhammad Saqib ◽  
Rui Hao
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Low Cost ◽  
Ionic Current ◽  
High Sensitivity ◽  
Protein Sequencing ◽  
Next Generation Sequencing Technology ◽  
Single Entity ◽  
Nanoparticle Detection ◽  
The Future

Nanopore-based single-entity detection shows immense potential in sensing and sequencing technologies. Solid-state nanopores permit unprecedented detail while preserving mechanical robustness, reusability, adjustable pore size, and stability in different physical and chemical environments. The transmission electron microscope (TEM) has evolved into a powerful tool for fabricating and characterizing nanometer-sized pores within a solid-state ultrathin membrane. By detecting differences in the ionic current signals due to single-entity translocation through the nanopore, solid-state nanopores can enable gene sequencing and single molecule/nanoparticle detection with high sensitivity, improved acquisition speed, and low cost. Here we briefly discuss the recent progress in the modification and characterization of TEM-fabricated nanopores. Moreover, we highlight some key applications of these nanopores in nucleic acids, protein, and nanoparticle detection. Additionally, we discuss the future of computer simulations in DNA and protein sequencing strategies. We also attempt to identify the challenges and discuss the future development of nanopore-detection technology aiming to promote the next-generation sequencing technology.

Nanometric depth phase imaging using low-cost on-chip lensless inline holographic microscopy

2020 ◽  
Vol 59 (10) ◽  
Author(s):  
Hanu Phani Ram Gurram ◽  
Ashwini S. Galande ◽  
Renu John
Keyword(s):  
Low Cost ◽  
Phase Imaging ◽  
Holographic Microscopy ◽  
On Chip
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