Nonlinear Ultrasound Propagation in Homogeneous and Heterogeneous Media: Factors Affecting the in situ Mechanical Index (MI)

Monodisperse polypyrrole/SBA-15 composite (PPy/SBA-15) was fabricated by in-situ polymerization and used for Cr(Ⅵ) adsorption from aqueous solution. PPy/SBA-15 was characterized by numerous approaches. Factors affecting the Cr(Ⅵ) adsorption process included...

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Analysis of TBM Tunnel Behavior in Jointed Rock Mass

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.90-93.2033 ◽

2011 ◽

Vol 90-93 ◽

pp. 2033-2036 ◽

Cited By ~ 1

Author(s):

Jin Shan Sun ◽

Hong Jun Guo ◽

Wen Bo Lu ◽

Qing Hui Jiang

Keyword(s):

Rock Mass ◽

Numerical Models ◽

Jointed Rock ◽

In Situ Stress ◽

Jointed Rock Mass ◽

Joint Orientation ◽

Joint Spacing ◽

Factors Affecting ◽

Dip Angle

The factors affecting the TBM tunnel behavior in jointed rock mass is investigated. In the numerical models the concrete segment lining of TBM tunnel is concerned, which is simulated as a tube neglecting the segment joint. And the TBM tunnel construction process is simulate considering the excavation and installing of the segment linings. Some cases are analyzed with different joint orientation, joint spacing, joint strength and tunnel depth. The results show that the shape and areas of loosing zones of the tunnel are influenced by the parameters of joint sets and in-situ stress significantly, such as dip angle, spacing, strength, and the in-situ stress statement. And the stress and deformation of the tunnel lining are influenced by the parameters of joint sets and in-situ stress, too.

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Factors Affecting Efficient Aquifer Restoration at In Situ Uranium Mine Sites

Groundwater Monitoring & Remediation ◽

10.1111/j.1745-6592.1990.tb00344.x ◽

1990 ◽

Vol 10 (2) ◽

pp. 107-112 ◽

Cited By ~ 8

Author(s):

James L. Osiensky ◽

Roy E. Williams

Keyword(s):

Uranium Mine ◽

Factors Affecting ◽

Mine Sites

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Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes

Applied and Environmental Microbiology ◽

10.1128/aem.03039-05 ◽

2006 ◽

Vol 72 (8) ◽

pp. 5311-5317 ◽

Cited By ~ 67

Author(s):

Kengo Kubota ◽

Akiyoshi Ohashi ◽

Hiroyuki Imachi ◽

Hideki Harada

Keyword(s):

In Situ Hybridization ◽

Nucleic Acid ◽

Fluorescence Intensity ◽

Dna Probes ◽

Locked Nucleic Acid ◽

Factors Affecting ◽

Hybridization Efficiency ◽

Probe Hybridization ◽

Major Factors

ABSTRACT Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. Fluorescently labeled LNA/DNA probes with two to four LNA substitutions exhibited strong fluorescence intensities equal to or greater than that of probe Eub338, although these probes did not show bright signals when they were synthesized as DNA probes; for example, the fluorescence intensity of probe Eco468 increased by 22-fold after three LNA bases were substituted for DNA bases. Dissociation profiles of the probes revealed that the dissociation temperature was directly related to the number of LNA substitutions and the fluorescence intensity. These results suggest that the introduction of LNA residues in DNA probes will be a useful approach for effectively enhancing probe hybridization efficiency.

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