Development of a PCR High‐Resolution Melt Assay for Artemisia absinthium (Wormwood) and a Triplex Assay with Two Additional “Unregulated Legal High” Species Datura stramonium (Jimson Weed) and Merremia tuberosa (Hawaiian Woodrose),

2019 ◽  
Vol 64 (6) ◽  
pp. 1817-1822
Author(s):  
Brianna D. Kiesel ◽  
Kelly M. Elkins
Keyword(s):  
High Resolution ◽  
Datura Stramonium ◽  
Artemisia Absinthium ◽  
High Resolution Melt ◽  
Jimson Weed ◽  
Legal High

scholarly journals High-resolution melt analysis to detect sequence variations in highly homologous gene regions: application to CYP2B6

2013 ◽  
Vol 14 (8) ◽  
pp. 913-922 ◽  
Author(s):  
Greyson P Twist ◽  
Roger Gaedigk ◽  
J Steven Leeder ◽  
Andrea Gaedigk
Keyword(s):  
High Resolution ◽  
Homologous Gene ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Sequence Variations

A rapid method for sex identification in Cannabis sativa using High Resolution Melt (HRM) analysis.

Botany ◽  
2021 ◽  
Author(s):  
Erin Jacqueline Gilchrist ◽  
Daniela Hegebarth ◽  
Shumin Wang ◽  
Teagen D. Quilichini ◽  
Jason Sawler ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Method ◽  
X Chromosome ◽  
Cannabis Sativa ◽  
Sex Identification ◽  
Male Plant ◽  
High Resolution Melt ◽  
Male And Female ◽  
High Resolution Melt Analysis ◽  
Plant Sex

We report the identification of two SNPs in Cannabis sativa that are associated with female and male plant sex phenotypes, and are located on the top arm of the X chromosome. High Resolution Melt analysis was used to develop and validate a novel, rapid method for sex identification in medical/recreational cannabis as well as in hemp. This method can distinguish between dioecious male (XY) and dioecious female (XX) cannabis plants with 100% accuracy, and can also be used to differentiate between male and female Humulus lupulus (hop) plants.

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