Testicular sperm retrieval: What should we expect from the fresh and subsequent cryopreserved sperm injection?

Andrologia ◽  
2020 ◽  
Author(s):  
Adva Aizer ◽  
Olga Dratviman‐Storobinsky ◽  
Meirav Noach‐Hirsh ◽  
Sarah Konopnicki ◽  
Alon Lazarovich ◽  
...  
Keyword(s):  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Testicular Sperm Retrieval ◽  
Cryopreserved Sperm

Successful testicular sperm retrieval in adolescents with Klinefelter syndrome treated with at least 1 year of topical testosterone and aromatase inhibitor

2013 ◽  
Vol 100 (4) ◽  
pp. 970-974 ◽  
Author(s):  
Akanksha Mehta ◽  
Alexander Bolyakov ◽  
Jordan Roosma ◽  
Peter N. Schlegel ◽  
Darius A. Paduch
Keyword(s):  
Aromatase Inhibitor ◽  
Klinefelter Syndrome ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Testicular Sperm Retrieval

scholarly journals USING GENE EXPRESSION PROFILES OF EJACULATES OF NOA MEN TO HELP PREDICT SUCCESSFUL TESTICULAR SPERM RETRIEVAL

2020 ◽  
Vol 114 (3) ◽  
pp. e366
Author(s):  
Brianne Barker ◽  
Alessandra Parrella ◽  
Zev Rosenwaks ◽  
Gianpiero D. Palermo
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Testicular Sperm Retrieval

scholarly journals Should diagnostic testicular sperm retrieval followed by cryopreservation for later ICSI be the procedure of choice for all patients with non-obstructive azoospermia?

2004 ◽  
Vol 19 (12) ◽  
pp. 2822-2830 ◽  
Author(s):  
G. Verheyen ◽  
V. Vernaeve ◽  
L. Van Landuyt ◽  
H. Tournaye ◽  
P. Devroey ◽  
...  
Keyword(s):  
Obstructive Azoospermia ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Testicular Sperm Retrieval

scholarly journals Testicular Damage following Testicular Sperm Retrieval: A Ram Model Study

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Jens Fedder ◽  
Niels Marcussen ◽  
Maja D. K. Fedder ◽  
Birte Engvad
Keyword(s):  
Glass Plate ◽  
Histological Evaluation ◽  
Testicular Damage ◽  
Testicular Sperm ◽  
Open Biopsy ◽  
Sperm Retrieval ◽  
Model Study ◽  
Testicular Sperm Retrieval

The aim of this study was to evaluate the possible development of histological abnormalities such as fibrosis and microcalcifications after sperm retrieval in a ram model. Fourteen testicles in nine rams were exposed to open biopsy, multiple TESAs, or TESE, and the remaining four testicles were left unoperated on as controls. Three months after sperm retrieval, the testicles were removed, fixed, and cut into 1/2 cm thick slices and systematically put onto a glass plate exposing macroscopic abnormalities. Tissue from abnormal areas was cut into 3 μm sections and stained for histological evaluation. Pathological abnormalities were observed in testicles exposed to sperm retrieval (≥11 of 14) compared to 0 of 4 control testicles. Testicular damage was found independently of the kind of intervention used. Therefore, cryopreservation of excess sperm should be considered while retrieving sperm.

Cytological Evaluation of Germinal Populations as Part of a Testicular Sperm Retrieval Strategy

1999 ◽  
pp. 253-258 ◽  
Author(s):  
Vincent Izard ◽  
Dominique Marmor ◽  
Anne Peron ◽  
Valérie Paradis ◽  
Gérard Benoît ◽  
...  
Keyword(s):  
Retrieval Strategy ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Testicular Sperm Retrieval ◽  
Cytological Evaluation

CDY1 and BOULE transcripts assessed in the same biopsy as predictive markers for successful testicular sperm retrieval

2011 ◽  
Vol 95 (7) ◽  
pp. 2297-2302.e1 ◽  
Author(s):  
Sandra E. Kleiman ◽  
Ofer Lehavi ◽  
Ron Hauser ◽  
Amnon Botchan ◽  
Gedalia Paz ◽  
...  
Keyword(s):  
Predictive Markers ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Testicular Sperm Retrieval

P–038 Clinical outcomes of 77 testicular sperm extraction treatment cycles in non-mosaic Klinefelter syndrome patients

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
P Barros ◽  
M Cunha ◽  
A Barros ◽  
S Dória ◽  
M Sousa
Keyword(s):  
Clinical Outcomes ◽  
Intracytoplasmic Sperm Injection ◽  
Klinefelter Syndrome ◽  
Hormone Replacement ◽  
Testicular Volume ◽  
Testicular Sperm Extraction ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Aneuploidy Screening ◽  
Testicular Sperm Retrieval

Abstract Study question What are the clinical results of patients with azoospermia and nonmosaic Klinefelter syndrome, using fresh and cryopreserved sperm? Summary answer The results showed a recovery rate of testicular sperm in the order of 40% and a life newborn rate of 52% when using fresh sperm What is known already In Klinefelter syndrome (KS), the rates of successful testicular sperm retrieval were shown to be similar either using conventional TESE or micro-TESE (Corona et al., 2017), which highlights that the variability observed between studies is due to differences in patient characteristics. There are a few works with a large number of KS patients elucidating the clinical outcomes using fresh and cryopreserved testicular sperm. However, these studies revealed contradictory outcomes, either revealing better (Greco et al., 2013; Vicdan et al., 2016) or worst (Madureira et al., 2014) results with cryopreserved testicular sperm, or finding no differences (Chen et al, 2019). Study design, size, duration This study includes all patients up to 2019 presenting azoospermia due to non-mosaic Klinefelter syndrome (n = 76) that went for infertility consultations in a private fertility clinic. Patients were evaluated by the same Urologist. The genetic analysis of the patients was performed at an academic institution. At examination patients did not refer other complaints besides infertility, and referred to have not received any hormone replacement therapy in the past. Participants/materials, setting, methods The 76 azoospermic patients with non-mosaic Klinefelter syndrome (KS) were treated by testicular sperm extraction (TESE) followed by intracytoplasmic sperm injection (ICSI), using fresh and cryopreserved testicular sperm. Most patients used fresh testicular sperm, where others preferred to postpone ICSI treatment cycles and used cryopreserved testicular sperm. Aneuploidy screening in children was performed by prenatal diagnosis and MLPA (Multiplex ligation-dependent probe amplification). Full embryological, clinical and newborn outcomes are provided. Main results and the role of chance Of the 76 patients with non-mosaic Klinefelter syndrome, one repeated the testicular sperm extraction (TESE) procedure. Testicular sperm were recovered in 31/77 (40.3%) of the cases. Comparisons between the 31 cases with successful sperm recover (group–1) and the 46 cases without a successful TESE (group–2) revealed no significant differences regarding age, time of infertility, testicular volume, serum levels of FSH, LH and testosterone, total number of testicular fragments analyzed, and time of search in samples. The mean male age was 34 years. In most of the cases, the testicular volume was reduced (96.1%), the levels of FSH (98.3%) and LH (94.1%) were increased, and the levels of testosterone were normal (77.6%). There were 25 intracytoplasmic sperm injection (ICSI) treatment cycles using fresh testicular sperm and 22 ICSI treatment cycles using frozen testicular sperm. The rates of fertilization (63.5% fresh sperm vs 41.6% frozen sperm), implantation (37% fresh sperm vs 13.2% frozen sperm), clinical pregnancy (60.9% fresh sperm vs 19% frozen sperm), live birth delivery (52.2% fresh sperm vs 19% frozen sperm) and newborn (65.2% fresh sperm vs 23.8% frozen sperm) were higher in the group using fresh testicular sperm. Chromosome analysis of the 21 newborn was normal. Limitations, reasons for caution Although presenting a high number of cases with azoospermic non-mosaic Klinefelter syndrome treated with testicular sperm extraction and intracytoplasmic sperm injection, future studies are needed with a higher number of cycles using frozen testicular sperm, in order to confirm or rebut that the freezing methodology affects negatively the clinical outcomes. Wider implications of the findings: Data adds further information regarding testicular sperm retrieval rates and use of fresh or frozen testicular sperm in Klinefelter syndrome (KS) patients. High newborn rates were obtained only with fresh testicular sperm. Results also reassure KS patients about the safety relative to any abnormal chromosomal transmission to the born children. Trial registration number Not applicable

Characteristics and outcomes of 499 consecutive cases of testicular sperm retrieval and ICSI for azoospermia in a Turkish state hospital-based art program

2013 ◽  
Vol 100 (3) ◽  
pp. S217
Author(s):  
S. Yalcinkaya ◽  
S. Keskin ◽  
T.M. Yalcinkaya ◽  
Z. Yildirim ◽  
O. Yucel
Keyword(s):  
State Hospital ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Art Program ◽  
Testicular Sperm Retrieval ◽  
Turkish State
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