Building a better mousetrap for accurate and sensitive polymerase chain reaction

2021 ◽  
Author(s):  
Jerry Radich ◽  
Fred Hutch
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Sensitive Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Molecular diagnosis of Beckwith-Wiedemann Syndrome using quantitative methylation-sensitive polymerase chain reaction

2006 ◽  
Vol 8 (10) ◽  
pp. 628-634 ◽  
Author(s):  
Bradford Coffee ◽  
Kasinathan Muralidharan ◽  
William E Highsmith ◽  
Pablo Lapunzina ◽  
Stephen T Warren
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Diagnosis ◽  
Chain Reaction ◽  
Sensitive Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Species-Specific Polymerase Chain Reaction Amplification of Camel (Camelus) DNA Extracts

2005 ◽  
Vol 88 (5) ◽  
pp. 1394-1398 ◽  
Author(s):  
Ying Chen ◽  
Ya-Jun Wu ◽  
Bao-Liang Xu ◽  
Jing Wan ◽  
Zeng-Ming Qian
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Chain Reaction ◽  
Pcr Product ◽  
Mitochondrial Cytochrome B ◽  
Base Pair Fragment ◽  
Sensitive Polymerase Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Species Specific

Abstract A sensitive polymerase chain reaction (PCR) method based on amplification of a specific DNA fragment was established for the identification of camel (Camelus) materials. The species-specific primer pair L183/H372 was designed based on the nucleotide sequence of the mitochondrial cytochrome b gene, and its specificity was confirmed by amplification of 3 camel (domestic double-humped camel, wild double-humped camel, wild one-humped camel) samples and 11 non-Camelus animal (sheep, goat, pig, chicken, cattle, fish, dog, horse, donkey, deer, and rabbit) materials. An expected 208 base pair fragment was amplified from camel materials; no cross-reactive or additional fragments were generated from other animal materials. Taq I restriction endonuclease digestion of the unpurified PCR product can be used routinely to confirm the camel origin of the amplified sequence.

Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay

Transfusion ◽  
2007 ◽  
Vol 47 (10) ◽  
pp. 1756-1764 ◽  
Author(s):  
Steven H. Kleinman ◽  
Simone A. Glynn ◽  
Tzong-Hae Lee ◽  
Leslie Tobler ◽  
Leilani Montalvo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Donors ◽  
Parvovirus B19 ◽  
Chain Reaction ◽  
Screening Assay ◽  
Sensitive Polymerase Chain Reaction ◽  
Polymerase Chain
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