scholarly journals In vivo tumor imaging using fluorescence conjugated anti-CEA antibody by two-photon excitation microscopy. A three-dimensional image of site inoculated with MKN-GFP gastric carcinoma cells preincubated with fluorescence conjugated anti-CEA antibody was ob

2014 ◽  
Vol 105 (10) ◽  
pp. October cover-October cover
Author(s):  
Shigehiro Koga ◽  
Yusuke Oshima ◽  
Naoki Honkura ◽  
Tadahiro Iimura ◽  
Kenji Kameda ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Tumor Imaging ◽  
Three Dimensional ◽  
Carcinoma Cells ◽  
Dimensional Image ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-Photon Excitation Imaging of Glucose Metabolism in Living Tissue

1997 ◽  
Vol 3 (S2) ◽  
pp. 305-306
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Collection Efficiency ◽  
Three Dimensional ◽  
Spatial Filter ◽  
Input Power ◽  
Photon Absorption ◽  
Focal Volume ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. It provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. This results in inherent submicron optical sectioning by excitation alone. In practice, TPEM is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 limits the average input power to less than 10 mW, only slightly greater than the power normally used in confocal microscopy. Because of the intensity-squared dependence of the two-photon absorption, the excitation is limited to the focal volume.

Crosstalk in Photoluminescence Readout of Three-Dimensional Memory in Vitreous Silica by One- and Two-Photon Excitation

2000 ◽  
Vol 39 (Part 1, No. 12A) ◽  
pp. 6763-6767 ◽  
Author(s):  
Mitsuru Watanabe ◽  
Saulius Juodkazis ◽  
Shigeki Matsuo ◽  
Junji Nishii ◽  
Hiroaki Misawa
Keyword(s):  
Three Dimensional ◽  
Vitreous Silica ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser

2020 ◽  
Vol 45 (10) ◽  
pp. 2704
Author(s):  
Ting Wu ◽  
Jiuling Liao ◽  
Jia Yu ◽  
Yufeng Gao ◽  
Hui Li ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Label Free ◽  
Two Photon ◽  
Photon Excitation ◽  
Femtosecond Fiber Laser ◽  
Two Photon Excitation

Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo

Bone ◽  
2015 ◽  
Vol 74 ◽  
pp. 134-139 ◽  
Author(s):  
Hiroshige Sano ◽  
Junichi Kikuta ◽  
Masayuki Furuya ◽  
Naoki Kondo ◽  
Naoto Endo ◽  
...  
Keyword(s):  
Bone Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Osteocytic Osteolysis ◽  
Two Photon Excitation

scholarly journals A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging

2005 ◽  
Vol 390 (3) ◽  
pp. 787-790 ◽  
Author(s):  
Stanley W. Botchway ◽  
Ignasi Barba ◽  
Randolf Jordan ◽  
Rebecca Harmston ◽  
Peter M. Haggie ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Emission ◽  
Yeast Cells ◽  
Fluorescent Label ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Novel Method ◽  
Two Photon Excitation

A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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