scholarly journals Characterizing the demographic history and prion protein variation to infer susceptibility to chronic wasting disease in a naïve population of white‐tailed deer ( Odocoileus virginianus )

2021 ◽  
Author(s):  
Sarah E. Haworth ◽  
Larissa Nituch ◽  
Joseph M. Northrup ◽  
Aaron B. A. Shafer
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Chronic Wasting Disease ◽  
Demographic History ◽  
Wasting Disease ◽  
Protein Variation

scholarly journals Association of chronic wasting disease susceptibility with prion protein variation in white-tailed deer (Odocoileus virginianus)

Prion ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 214-225 ◽  
Author(s):  
Yasuko Ishida ◽  
Ting Tian ◽  
Adam L. Brandt ◽  
Amy C. Kelly ◽  
Paul Shelton ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Disease Susceptibility ◽  
Chronic Wasting Disease ◽  
Wasting Disease ◽  
Protein Variation

scholarly journals Prion protein gene sequence and chronic wasting disease susceptibility in white-tailed deer (Odocoileus virginianus)

Prion ◽  
2015 ◽  
Vol 9 (6) ◽  
pp. 449-462 ◽  
Author(s):  
Adam L Brandt ◽  
Amy C Kelly ◽  
Michelle L Green ◽  
Paul Shelton ◽  
Jan Novakofski ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Gene Sequence ◽  
Disease Susceptibility ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Prion Protein Gene ◽  
Wasting Disease

scholarly journals Prion Protein Gene (PRNP) Sequences Suggest Differing Vulnerability to Chronic Wasting Disease for Florida Key Deer (Odocoileus virginianus clavium) and Columbian White-Tailed Deer (O. v. leucurus)

2020 ◽  
Vol 111 (6) ◽  
pp. 564-572
Author(s):  
Tolulope I N Perrin-Stowe ◽  
Yasuko Ishida ◽  
Emily E Terrill ◽  
Brian C Hamlin ◽  
Linda Penfold ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Synonymous Substitution ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Genetic Vulnerability ◽  
Key Deer

Abstract Chronic wasting disease (CWD) is a fatal, highly transmissible spongiform encephalopathy caused by an infectious prion protein. CWD is spreading across North American cervids. Studies of the prion protein gene (PRNP) in white-tailed deer (WTD; Odocoileus virginianus) have identified non-synonymous substitutions associated with reduced CWD frequency. Because CWD is spreading rapidly geographically, it may impact cervids of conservation concern. Here, we examined the genetic vulnerability to CWD of 2 subspecies of WTD: the endangered Florida Key deer (O. v. clavium) and the threatened Columbian WTD (O. v. leucurus). In Key deer (n = 48), we identified 3 haplotypes formed by 5 polymorphisms, of which 2 were non-synonymous. The polymorphism c.574G>A, unique to Key deer (29 of 96 chromosomes), encodes a non-synonymous substitution from valine to isoleucine at codon 192. In 91 of 96 chromosomes, Key deer carried c.286G>A (G96S), previously associated with substantially reduced susceptibility to CWD. Key deer may be less genetically susceptible to CWD than many mainland WTD populations. In Columbian WTD (n = 13), 2 haplotypes separated by one synonymous substitution (c.438C>T) were identified. All of the Columbian WTD carried alleles that in other mainland populations are associated with relatively high susceptibility to CWD. While larger sampling is needed, future management plans should consider that Columbian WTD are likely to be genetically more vulnerable to CWD than many other WTD populations. Finally, we suggest that genetic vulnerability to CWD be assessed by sequencing PRNP across other endangered cervids, both wild and in captive breeding facilities.

scholarly journals Prion protein in cardiac muscle of elk (Cervus elaphus nelsoni) and white-tailed deer (Odocoileus virginianus) infected with chronic wasting disease

2006 ◽  
Vol 87 (11) ◽  
pp. 3443-3450 ◽  
Author(s):  
Jean E. Jewell ◽  
Jeremy Brown ◽  
Terry Kreeger ◽  
Elizabeth S. Williams
Keyword(s):  
Western Blot ◽  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Cervus Elaphus ◽  
Chronic Wasting Disease ◽  
Striated Muscle ◽  
Mule Deer ◽  
Spongiform Encephalopathy ◽  
Wasting Disease ◽  
Cervus Elaphus Nelsoni

To investigate the possible presence of disease-associated prion protein (PrPd) in striated muscle of chronic wasting disease (CWD)-affected cervids, samples of diaphragm, tongue, heart and three appendicular skeletal muscles from mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), elk (Cervus elaphus nelsoni) and moose (Alces alces shirasi) were examined by ELISA, Western immunoblot and immunohistochemistry (IHC). PrPd was detected in samples of heart muscle from seven of 16 CWD-infected white-tailed deer, including one free-ranging deer, and in 12 of 17 CWD-infected elk, but not in any of 13 mule deer samples, nor in the single CWD-infected moose. For white-tailed deer, PrPd was detected by Western blot at multiple sites throughout the heart; IHC results on ventricular sections of both elk and white-tailed deer showed positive staining in cardiac myocytes, but not in conduction tissues or nerve ganglia. Levels of PrPd in cardiac tissues were estimated from Western blot band intensity to be lower than levels found in brain tissue. PrPd was not detected in diaphragm, triceps brachii, semitendinosus, latissiumus dorsi or tongue muscles for any of the study subjects. This is the first report of PrPd in cardiac tissue from transmissible spongiform encephalopathy-infected ruminants in the human food chain and the first demonstration by immunological assays of PrPd in any striated muscle of CWD-infected cervids.

scholarly journals Efficient In Vitro Amplification of Chronic Wasting Disease PrPRES

2007 ◽  
Vol 81 (17) ◽  
pp. 9605-9608 ◽  
Author(s):  
Timothy D. Kurt ◽  
Matthew R. Perrott ◽  
Carol J. Wilusz ◽  
Jeffrey Wilusz ◽  
Surachai Supattapone ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Prion Protein ◽  
Protein Misfolding ◽  
Chronic Wasting Disease ◽  
Body Fluids ◽  
The Body ◽  
Wasting Disease ◽  
Highly Efficient ◽  
Significant Step

ABSTRACT Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPC, to a protease-resistant conformer, PrPCWD. Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrPCWD in vitro. Normal brains from deer, transgenic mice expressing cervid PrPC [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrPC substrate produced >6.5 × 109-fold amplification after six rounds. Highly efficient in vitro amplification of PrPCWD is a significant step toward detection of PrPCWD in the body fluids or excreta of CWD-susceptible species.

scholarly journals Recombinant prion protein vaccination of transgenic elk PrP mice and reindeer overcomes self-tolerance and protects mice against chronic wasting disease

2018 ◽  
Vol 293 (51) ◽  
pp. 19812-19822 ◽  
Author(s):  
Dalia H. Abdelaziz ◽  
Simrika Thapa ◽  
Jenna Brandon ◽  
Justine Maybee ◽  
Lauren Vankuppeveld ◽  
...  
Keyword(s):  
Prion Protein ◽  
Chronic Wasting Disease ◽  
Wasting Disease ◽  
Self Tolerance ◽  
Recombinant Prion Protein

Species barriers for chronic wasting disease by in vitro conversion of prion protein

2007 ◽  
Vol 364 (4) ◽  
pp. 796-800 ◽  
Author(s):  
Li Li ◽  
Michael B. Coulthart ◽  
Aru Balachandran ◽  
Avi Chakrabartty ◽  
Neil R. Cashman
Keyword(s):  
Prion Protein ◽  
Chronic Wasting Disease ◽  
Wasting Disease
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