scholarly journals EFFECT OF ORAL COPPER SUPPLEMENTATION ON SUSCEPTIBILITY IN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) TO CHRONIC WASTING DISEASE

2020 ◽  
Vol 56 (3) ◽  
pp. 568
Author(s):  
Lisa L. Wolfe ◽  
Mary M. Conner ◽  
Michael W. Miller
Keyword(s):  
Odocoileus Virginianus ◽  
Chronic Wasting Disease ◽  
Wasting Disease ◽  
Copper Supplementation

scholarly journals Prion protein gene sequence and chronic wasting disease susceptibility in white-tailed deer (Odocoileus virginianus)

Prion ◽  
2015 ◽  
Vol 9 (6) ◽  
pp. 449-462 ◽  
Author(s):  
Adam L Brandt ◽  
Amy C Kelly ◽  
Michelle L Green ◽  
Paul Shelton ◽  
Jan Novakofski ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Gene Sequence ◽  
Disease Susceptibility ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Prion Protein Gene ◽  
Wasting Disease

scholarly journals Prion Protein Gene (PRNP) Sequences Suggest Differing Vulnerability to Chronic Wasting Disease for Florida Key Deer (Odocoileus virginianus clavium) and Columbian White-Tailed Deer (O. v. leucurus)

2020 ◽  
Vol 111 (6) ◽  
pp. 564-572
Author(s):  
Tolulope I N Perrin-Stowe ◽  
Yasuko Ishida ◽  
Emily E Terrill ◽  
Brian C Hamlin ◽  
Linda Penfold ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Protein Gene ◽  
Chronic Wasting Disease ◽  
Synonymous Substitution ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Wasting Disease ◽  
Genetic Vulnerability ◽  
Key Deer

Abstract Chronic wasting disease (CWD) is a fatal, highly transmissible spongiform encephalopathy caused by an infectious prion protein. CWD is spreading across North American cervids. Studies of the prion protein gene (PRNP) in white-tailed deer (WTD; Odocoileus virginianus) have identified non-synonymous substitutions associated with reduced CWD frequency. Because CWD is spreading rapidly geographically, it may impact cervids of conservation concern. Here, we examined the genetic vulnerability to CWD of 2 subspecies of WTD: the endangered Florida Key deer (O. v. clavium) and the threatened Columbian WTD (O. v. leucurus). In Key deer (n = 48), we identified 3 haplotypes formed by 5 polymorphisms, of which 2 were non-synonymous. The polymorphism c.574G>A, unique to Key deer (29 of 96 chromosomes), encodes a non-synonymous substitution from valine to isoleucine at codon 192. In 91 of 96 chromosomes, Key deer carried c.286G>A (G96S), previously associated with substantially reduced susceptibility to CWD. Key deer may be less genetically susceptible to CWD than many mainland WTD populations. In Columbian WTD (n = 13), 2 haplotypes separated by one synonymous substitution (c.438C>T) were identified. All of the Columbian WTD carried alleles that in other mainland populations are associated with relatively high susceptibility to CWD. While larger sampling is needed, future management plans should consider that Columbian WTD are likely to be genetically more vulnerable to CWD than many other WTD populations. Finally, we suggest that genetic vulnerability to CWD be assessed by sequencing PRNP across other endangered cervids, both wild and in captive breeding facilities.

scholarly journals Prion protein in cardiac muscle of elk (Cervus elaphus nelsoni) and white-tailed deer (Odocoileus virginianus) infected with chronic wasting disease

2006 ◽  
Vol 87 (11) ◽  
pp. 3443-3450 ◽  
Author(s):  
Jean E. Jewell ◽  
Jeremy Brown ◽  
Terry Kreeger ◽  
Elizabeth S. Williams
Keyword(s):  
Western Blot ◽  
Odocoileus Virginianus ◽  
Prion Protein ◽  
Cervus Elaphus ◽  
Chronic Wasting Disease ◽  
Striated Muscle ◽  
Mule Deer ◽  
Spongiform Encephalopathy ◽  
Wasting Disease ◽  
Cervus Elaphus Nelsoni

To investigate the possible presence of disease-associated prion protein (PrPd) in striated muscle of chronic wasting disease (CWD)-affected cervids, samples of diaphragm, tongue, heart and three appendicular skeletal muscles from mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), elk (Cervus elaphus nelsoni) and moose (Alces alces shirasi) were examined by ELISA, Western immunoblot and immunohistochemistry (IHC). PrPd was detected in samples of heart muscle from seven of 16 CWD-infected white-tailed deer, including one free-ranging deer, and in 12 of 17 CWD-infected elk, but not in any of 13 mule deer samples, nor in the single CWD-infected moose. For white-tailed deer, PrPd was detected by Western blot at multiple sites throughout the heart; IHC results on ventricular sections of both elk and white-tailed deer showed positive staining in cardiac myocytes, but not in conduction tissues or nerve ganglia. Levels of PrPd in cardiac tissues were estimated from Western blot band intensity to be lower than levels found in brain tissue. PrPd was not detected in diaphragm, triceps brachii, semitendinosus, latissiumus dorsi or tongue muscles for any of the study subjects. This is the first report of PrPd in cardiac tissue from transmissible spongiform encephalopathy-infected ruminants in the human food chain and the first demonstration by immunological assays of PrPd in any striated muscle of CWD-infected cervids.

scholarly journals Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)

2021 ◽  
Author(s):  
David Minich ◽  
Christopher Madden ◽  
Morgan V. Evans ◽  
Gregory A. Ballash ◽  
Daniel J. Barr ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Odocoileus Virginianus ◽  
Chronic Wasting Disease ◽  
16S Rrna Gene Sequencing ◽  
The Body ◽  
Rrna Gene ◽  
Wasting Disease ◽  
Indirect Contact ◽  
Rrna Gene Sequencing ◽  
Free Ranging

AbstractChronic wasting disease (CWD) is a fatal, contagious, neurodegenerative prion disease affecting both free-ranging and captive cervid species. CWD is spread via direct or indirect contact or oral ingestion of prions. In the gastrointestinal tract, prions enter the body through microfold cells (M-cells), and the abundance of these cells can be influenced by the gut microbiota. To explore potential links between the gut microbiota and CWD, we collected fecal samples from farmed and free-ranging white-tailed deer (Odocoileus virginianus) around the Midwest. Farmed deer orignated from farms that were depopulated due to CWD. Free-ranging deer were sampled during annual deer harvests. All farmed deer were tested for CWD via ELISA and IHC, and we used 16S rRNA gene sequencing to characterize the gut microbiota. We report significant differences in gut microbiota by provenance (Farm 1, Farm 2, Free-ranging), sex, and CWD status. CWD-positive deer from Farm 1 and 2 had increased abundances of Akkermansia, Lachnospireacea UCG-010, and RF39 taxa. Overall, differences by provenance and sex appear to be driven by diet, while differences by CWD status may be linked to CWD pathogenesis.

scholarly journals In Vitro detection of Chronic Wasting Disease (CWD) prions in semen and reproductive tissues of white tailed deer bucks (Odocoileus virginianus)

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0226560 ◽  
Author(s):  
Carlos Kramm ◽  
Ruben Gomez-Gutierrez ◽  
Claudio Soto ◽  
Glenn Telling ◽  
Tracy Nichols ◽  
...  
Keyword(s):  
Odocoileus Virginianus ◽  
Chronic Wasting Disease ◽  
Wasting Disease ◽  
Reproductive Tissues
Sign in / Sign up

Export Citation Format

Share Document