Efficient In Vitro Amplification of Chronic Wasting Disease PrPRES
Keyword(s):
The Body
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ABSTRACT Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPC, to a protease-resistant conformer, PrPCWD. Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrPCWD in vitro. Normal brains from deer, transgenic mice expressing cervid PrPC [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrPC substrate produced >6.5 × 109-fold amplification after six rounds. Highly efficient in vitro amplification of PrPCWD is a significant step toward detection of PrPCWD in the body fluids or excreta of CWD-susceptible species.
2017 ◽
2007 ◽
Vol 364
(4)
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pp. 796-800
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2012 ◽
Vol 24
(4)
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pp. 746-749
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Keyword(s):
2006 ◽
Vol 80
(2)
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pp. 596-604
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Keyword(s):