scholarly journals The non‐homogenous distribution and aggregation of carotenoids in the stratum corneum correlates with the organization of intercellular lipids in vivo

2019 ◽  
Vol 28 (11) ◽  
pp. 1237-1243 ◽  
Author(s):  
ChunSik Choe ◽  
JaeRyong Ri ◽  
Johannes Schleusener ◽  
Juergen Lademann ◽  
Maxim E. Darvin
Keyword(s):  
Stratum Corneum ◽  
Homogenous Distribution ◽  
Intercellular Lipids

scholarly journals Current views on non-invasive in vivo determination of physiological parameters of the stratum corneum using confocal Raman microspectroscopy

2022 ◽  
Author(s):  
Maxim E. Darvin ◽  
Johannes Schleusener ◽  
Jürgen Lademann ◽  
Chun-Sik Choe
Keyword(s):  
Stratum Corneum ◽  
Raman Microspectroscopy ◽  
Physiological Parameters ◽  
Skin Barrier Function ◽  
Non Invasive ◽  
Intercellular Lipids ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Confocal Raman microspectroscopy is widely used in dermatology and cosmetology for analysis of the concentration of skin components (lipids, natural moisturizing factor molecules, water) and the penetration depth of cosmetic/medical formulations in the human stratum corneum (SC) in vivo. In recent years, it was shown that confocal Raman microspectroscopy can also be used for non-invasive in vivo depth-dependent determination of the physiological parameters of the SC, such as lamellar and lateral organization of intercellular lipids, folding properties of keratin, water mobility and hydrogen bonding states. The results showed that the strongest skin barrier function, which is primarily manifested by the orthorhombic organization of intercellular lipids, is provided at ≈20–40% SC depth, which is related to the maximal bonding state of water with surrounding components in the SC. The secondary and tertiary structures of keratin determine water binding in the SC, which is depth-dependent. This paper shows the technical possibility and advantage of confocal Raman microspectroscopy in non-invasive investigation of the skin and summarizes recent results on in vivo investigation of the human SC.

scholarly journals Liposome percutaneous penetration in vivo

2017 ◽  
Vol 1 ◽  
pp. 239784731772319 ◽  
Author(s):  
A Lymberopoulos ◽  
C Demopoulou ◽  
M Kyriazi ◽  
MS Katsarou ◽  
N Demertzis ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Rhodamine B ◽  
Transdermal Delivery ◽  
Penetration Enhancers ◽  
Percutaneous Penetration ◽  
Hairless Mice ◽  
Liposomal Form ◽  
Multilamellar Vesicles ◽  
Small Unilamellar Vesicles

Objectives: Liposomes are reported as penetration enhancers for dermal and transdermal delivery. However, little is known about their percutaneous penetration and as to at which level they deliver encapsulated drugs. The penetration of multilamellar vesicles (MLVs) and small unilamellar vesicles (SUVs), in comparison to one of their lipid components, was investigated. Methods: Using the fluorescent lipid, Lissamine Rhodamine B-PE (R), as a constituent, MLV and SUV liposomes were prepared, tested, and R, MLV, or SUV were applied in vivo on the back of hairless mice. Absorption of each was evaluated at the levels of stratum corneum, living skin, and blood by fluorometry. Results: Penetration of the lipid R in stratum corneum in the nonliposomal form exceeded that in the liposomal form and only R penetrates the living skin in a statistically significant manner. No statistical significant absorption into blood was observed with either form. Conclusions: Liposomes size did not play an important role in penetration to stratum corneum. The lipid constituent in the nonliposomal form penetrated at higher rates into stratum corneum and living skin. Even though these liposomes entered stratum corneum, they were not significantly absorbed into viable skin or blood.

Human Skin Binding and Absorption of Contaminants from Ground and Surface Water During Swimming and Bathing

1989 ◽  
Vol 8 (5) ◽  
pp. 853-859 ◽  
Author(s):  
Ronald C. Wester ◽  
Howard I. Maibach
Keyword(s):  
Surface Water ◽  
Stratum Corneum ◽  
Human Skin ◽  
The Body ◽  
Water Soluble ◽  
Exposure Effect ◽  
Human Stratum Corneum ◽  
Water Dilution

Contaminants exist in ground and surface water. Human skin has the capacity to bind and then absorb these contaminants into the body during swimming and bathing. Powdered human stratum corneum will bind both lipid-soluble (alachlor, polychlorinated biphenyls [PCBs], benzene) and water-soluble (nitroaniline) chemicals. In vitro (human skin) and in vivo (Rhesus monkey) studies show that these chemicals readily distribute into skin, and then some of the chemical is absorbed into the body. Linearity in binding and absorption exists for nitroaniline over a 10-fold concentration range. Multiple exposure to benzene is at least cumulative. Binding and absorption can be significant for exposures as short as 30 min, and will increase with time. Absorption with water dilution increased for alachlor, but not for dinoseb. Soap reversed the partitioning of alachlor between human stratum corneum and water. The PCBs could be removed from skin by soap and water (70% efficiency) for up to 3 h and then decontamination potential decreased, due to continuing skin absorption. The model in vitro and in vivo systems used should permit easy estimation of this area of extensive human exposure effect on risk assessment.

scholarly journals Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

2019 ◽  
Vol 10 (6) ◽  
pp. 3092 ◽  
Author(s):  
Maxim E. Darvin ◽  
Chun-Sik Choe ◽  
Johannes Schleusener ◽  
Jürgen Lademann
Keyword(s):  
Stratum Corneum ◽  
Depth Profiling ◽  
Raman Microscopy ◽  
Homogeneous Distribution ◽  
Confocal Raman Microscopy ◽  
Non Invasive ◽  
Confocal Raman

scholarly journals The Effect of in Vivo Occlusion on Human Stratum Corneum Hydration-Dehydration in Vitro

1973 ◽  
Vol 61 (6) ◽  
pp. 375-379 ◽  
Author(s):  
Robert L. Anderson ◽  
Jean M. Cassidy ◽  
John R. Hansen ◽  
Wilbur Yellin
Keyword(s):  
Stratum Corneum ◽  
Human Stratum Corneum ◽  
Stratum Corneum Hydration

Physical Methods of Measuring Stratum Corneum Water Content in Vivo

2004 ◽  
pp. 112-152 ◽  
Author(s):  
Jean-Claude Bernengo ◽  
Jean de Rigal
Keyword(s):  
Stratum Corneum ◽  
Water Content ◽  
Physical Methods ◽  
Stratum Corneum Water Content

Determination in vivo of water concentration profile in human stratum corneum by a photoacoustic method

1981 ◽  
pp. 187-195 ◽  
Author(s):  
I. Simon ◽  
A. G. Emslie ◽  
C. M. Apt ◽  
I. H. Blank ◽  
R. R. Anderson
Keyword(s):  
Stratum Corneum ◽  
Concentration Profile ◽  
Water Concentration ◽  
Human Stratum Corneum ◽  
Photoacoustic Method

scholarly journals In vivo Tracking of DNA for Precise Determination of the Stratum Corneum Thickness and Superficial Microbiome Using Confocal Raman Microscopy

2019 ◽  
Vol 33 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Jin Song Ri ◽  
Se Hyok Choe ◽  
Johannes Schleusener ◽  
Jürgen Lademann ◽  
Chun Sik Choe ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Precise Determination ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Confocal Raman
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