scholarly journals Structural characterization of the C-terminal coiled-coil domains of wild-type and kidney disease-associated mutants of apolipoprotein L1

FEBS Journal ◽  
2016 ◽  
Vol 283 (10) ◽  
pp. 1846-1862 ◽  
Author(s):  
Alok K. Sharma ◽  
David J. Friedman ◽  
Martin R. Pollak ◽  
Seth L. Alper
Keyword(s):  
Kidney Disease ◽  
Structural Characterization ◽  
Coiled Coil ◽  
Wild Type ◽  
Apolipoprotein L1

scholarly journals 2066 Functional characterization of mutant BRCA1

2018 ◽  
Vol 2 (S1) ◽  
pp. 13-13
Author(s):  
John Barrows ◽  
David Long
Keyword(s):  
Functional Characterization ◽  
Coiled Coil ◽  
Wild Type ◽  
Coiled Coil Region ◽  
Egg Extracts ◽  
Study Population ◽  
Xenopus Egg ◽  
Xenopus Egg Extracts

OBJECTIVES/SPECIFIC AIMS: The objective of this work is to determine the mechanistic consequences of BRCA1 mutants in inter-strand crosslink (ICL) repair. METHODS/STUDY POPULATION: Our lab uses Xenopus egg extracts to study ICL repair. These extracts can be depleted of endogenous BRCA1 by immunoprecipitation. The goal of this work is to rescue endogenous depletion with in vitro translated, wild type BRCA1. Once achieved, we can supplement the depleted extract with BRCA1 mutants to access their function in ICL repair. RESULTS/ANTICIPATED RESULTS: We hypothesize that the BRCT and RING domain mutations will abrogate ICL repair, while mutations in the coiled coil region will not affect repair. DISCUSSION/SIGNIFICANCE OF IMPACT: These findings will have an immense impact on the understanding of BRCA1 domains. Importantly these results will spur personalized therapy of BRCA1 mutants by showing which domains are sensitive to cross-linking agents.

Tumor Suppressor p16INK4A:  Structural Characterization of Wild-Type and Mutant Proteins by NMR and Circular Dichroism†

Biochemistry ◽  
1996 ◽  
Vol 35 (29) ◽  
pp. 9475-9487 ◽  
Author(s):  
Anton Tevelev ◽  
In-Ja L. Byeon ◽  
Thomas Selby ◽  
Karen Ericson ◽  
Hee-Jung Kim ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Tumor Suppressor ◽  
Structural Characterization ◽  
Wild Type ◽  
Mutant Proteins ◽  
Circular Dichroïsm

scholarly journals Structural Characterization of an LPA1 Second Extracellular Loop Mimetic with a Self-Assembling Coiled-Coil Folding Constraint

2013 ◽  
Vol 14 (2) ◽  
pp. 2788-2807 ◽  
Author(s):  
John Young ◽  
Benjamin Clayton ◽  
Alexandra Kikonyogo ◽  
Truc-Chi Pham ◽  
Abby Parrill
Keyword(s):  
Structural Characterization ◽  
Coiled Coil ◽  
Extracellular Loop ◽  
Self Assembling

Solution Structural Characterization of Coiled-Coil Peptide–Polymer Side-Conjugates

2012 ◽  
Vol 13 (6) ◽  
pp. 1945-1955 ◽  
Author(s):  
Jessica Y. Shu ◽  
Reidar Lund ◽  
Ting Xu
Keyword(s):  
Structural Characterization ◽  
Coiled Coil

Metal-binding properties and structural characterization of a self-assembled coiled coil: Formation of a polynuclear Cd–thiolate cluster

2013 ◽  
Vol 119 ◽  
pp. 1-9 ◽  
Author(s):  
Daniil V. Zaytsev ◽  
Vasily A. Morozov ◽  
Jiufeng Fan ◽  
Xianchun Zhu ◽  
Madhumita Mukherjee ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Metal Binding ◽  
Coiled Coil ◽  
Binding Properties ◽  
Self Assembled

Structural characterization of pellicle polysaccharides of Acetobacter tropicalis SKU1100 wild type and mutant strains

2011 ◽  
Vol 86 (2) ◽  
pp. 1000-1006 ◽  
Author(s):  
Ibnaof Ali Ibnaof Ali ◽  
Yoshihiko Akakabe ◽  
Somporn Moonmangmee ◽  
Arpaporn Deeraksa ◽  
Minenosuke Matsutani ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Wild Type ◽  
Mutant Strains

scholarly journals Structural Characterization of Lignocresols from Transgenic and Wild-Type Switchgrass

Polymers ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 727 ◽  
Author(s):  
Hao Ren ◽  
Wenyuan Tian ◽  
Fan Shu ◽  
Dongliang Xu ◽  
Chunxiang Fu ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Wild Type
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