scholarly journals CRISPR screening strategies for microRNA target identification

FEBS Journal ◽  
2020 ◽  
Vol 287 (14) ◽  
pp. 2914-2922 ◽  
Author(s):  
Bing Yang ◽  
Katherine McJunkin
Keyword(s):  
Target Identification ◽  
Microrna Target ◽  
Screening Strategies

Human microRNA target identification by RRSM

2011 ◽  
Vol 286 ◽  
pp. 79-84 ◽  
Author(s):  
Wan J. Hsieh ◽  
Hsiuying Wang
Keyword(s):  
Target Identification ◽  
Microrna Target

Screening Strategies for Drug Discovery and Target Identification

2015 ◽  
pp. 135-166 ◽  
Author(s):  
Fatma Al-­Awadhi ◽  
Lilibeth Salvador ◽  
Hendrik Luesch
Keyword(s):  
Drug Discovery ◽  
Target Identification ◽  
Screening Strategies

scholarly journals MicroRNA Target Identification—Experimental Approaches

Biology ◽  
2013 ◽  
Vol 2 (1) ◽  
pp. 189-205 ◽  
Author(s):  
Aida Martinez-Sanchez ◽  
Chris Murphy
Keyword(s):  
Target Identification ◽  
Microrna Target ◽  
Experimental Approaches

scholarly journals Experimental strategies for microRNA target identification

2011 ◽  
Vol 39 (16) ◽  
pp. 6845-6853 ◽  
Author(s):  
D. W. Thomson ◽  
C. P. Bracken ◽  
G. J. Goodall
Keyword(s):  
Target Identification ◽  
Microrna Target ◽  
Experimental Strategies

scholarly journals Contemporary Ribonomics Methods for Viral microRNA Target Analysis

2018 ◽  
Vol 4 (4) ◽  
pp. 31 ◽  
Author(s):  
Lauren Gay ◽  
Peter Turner ◽  
Rolf Renne
Keyword(s):  
High Throughput Sequencing ◽  
Rna Binding ◽  
Cultured Cells ◽  
Target Identification ◽  
Microrna Target ◽  
Link Protein ◽  
Barr Virus ◽  
Cellular Processes ◽  
Viral Microrna ◽  
Epstein Barr

Numerous cellular processes are regulated by microRNAs (miRNAs), both cellular and viral. Elucidating the targets of miRNAs has become an active area of research. An important method in this field is cross-linking and immunoprecipitation (CLIP), where cultured cells or tissues are UV-irradiated to cross-link protein and nucleic acid, the RNA binding protein of interest is immunoprecipitated, and the RNAs pulled down with the protein are isolated, reverse-transcribed, and analyzed by sequencing. CLIP using antibody against Argonaute (Ago), which binds to both miRNA and mRNA as they interact in RISC, has allowed researchers to uncover a large number of miRNA targets. Coupled with high-throughput sequencing, CLIP has been useful for revealing miRNA targetomes for the γ-herpesviruses Kaposi’s sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV). Variants on the CLIP protocol are described, with the benefits and drawbacks of each. In particular, the most recent methods involving RNA–RNA ligation to join the miRNA and its RNA target have aided in target identification. Lastly, data supporting biologically meaningful interactions between miRNAs and long non-coding RNAs (lncRNAs) are reviewed. In summary, ribonomics-based miRNA targetome analysis has expanded our understanding of miRNA targeting and has provided a rich resource for EBV and KSHV research with respect to pathogenesis and tumorigenesis.

MicroRNA Target Identification: Lessons from HypoxamiRs

2014 ◽  
Vol 21 (8) ◽  
pp. 1249-1268 ◽  
Author(s):  
Thomas Bertero ◽  
Karine Robbe-Sermesant ◽  
Kevin Le Brigand ◽  
Gilles Ponzio ◽  
Nicolas Pottier ◽  
...  
Keyword(s):  
Target Identification ◽  
Microrna Target

scholarly journals Statistical Use of Argonaute Expression and RISC Assembly in microRNA Target Identification

2009 ◽  
Vol 5 (9) ◽  
pp. e1000516 ◽  
Author(s):  
Stephen A. Stanhope ◽  
Srikumar Sengupta ◽  
Johan den Boon ◽  
Paul Ahlquist ◽  
Michael A. Newton
Keyword(s):  
Target Identification ◽  
Microrna Target

Transfected Cell Lines as Tools for High Throughput Screening: A Call for Standards

2001 ◽  
Vol 6 (3) ◽  
pp. 133-136 ◽  
Author(s):  
Len Pagliaro ◽  
Morten Præstegaard
Keyword(s):  
Cell Lines ◽  
High Throughput ◽  
High Throughput Screening ◽  
Target Identification ◽  
Complex Variables ◽  
Screening System ◽  
Library Size ◽  
Global Issues ◽  
Screening Strategies ◽  
Assay Design

During 1999, Journal of Biomolecular Screening presented a series of Point-Counterpoint articles that addressed a question posed by editor Bill Janzen: "What is the future of HTS?" These articles discussed many of the global issues involved in HTS, such as target identification and library size, as well as the scientific and technical challenges facing the field. In this perspective we address a related, but very focused, issue that is increasingly important for many of us in the HTS community: the use of stably transfected cell lines as an integral part of screening strategies. Transfected cell lines provide powerful tools for assay design, but at the same time they introduce complex variables into the screening system. Although it is difficult to develop precise definitions and standards for biologicals such as cell lines, we propose that the development of guidelines for the nomenclature and use of transfected cell lines is essential for their use in HTS.

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