Phosphatidylserine flux into mitochondria unveiled by organelle‐targeted Escherichia coli phosphatidylserine synthase PssA

FEBS Journal ◽  
2020 ◽  
Author(s):  
Hiroya Shiino ◽  
Shiina Furuta ◽  
Rieko Kojima ◽  
Keisuke Kimura ◽  
Toshiya Endo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phosphatidylserine Synthase

scholarly journals Substrate-induced membrane association of phosphatidylserine synthase from Escherichia coli.

1986 ◽  
Vol 165 (3) ◽  
pp. 805-812 ◽  
Author(s):  
K Louie ◽  
Y C Chen ◽  
W Dowhan
Keyword(s):  
Escherichia Coli ◽  
Membrane Association ◽  
Induced Membrane ◽  
Phosphatidylserine Synthase

scholarly journals Phosphatidylserine synthase from Escherichia coli. The role of Triton X-100 in catalysis.

1979 ◽  
Vol 254 (17) ◽  
pp. 8391-8397
Author(s):  
G.M. Carman ◽  
W. Dowhan
Keyword(s):  
Escherichia Coli ◽  
Phosphatidylserine Synthase ◽  
Triton X

Phospholipids chiral at phosphorus. Steric course of the reactions catalyzed by phosphatidylserine synthase from Escherichia coli and yeast

Biochemistry ◽  
1987 ◽  
Vol 26 (13) ◽  
pp. 4022-4027 ◽  
Author(s):  
Christian R. H. Raetz ◽  
George M. Carman ◽  
William Dowhan ◽  
Ru Tai Jiang ◽  
Wieslaw Waszkuc ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phosphatidylserine Synthase

scholarly journals Phosphatidylethanolamine Domains and Localization of Phospholipid Synthases in Bacillus subtilis Membranes

2005 ◽  
Vol 187 (6) ◽  
pp. 2163-2174 ◽  
Author(s):  
Ayako Nishibori ◽  
Jin Kusaka ◽  
Hiroshi Hara ◽  
Masato Umeda ◽  
Kouji Matsumoto
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Green Fluorescent Protein ◽  
Acridine Orange ◽  
Fluorescent Protein ◽  
Cyclic Peptide ◽  
Peptide Probe ◽  
Phosphatidylserine Synthase ◽  
Green Fluorescent ◽  
Mutant Cells

ABSTRACT Application of the cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange has recently revealed CL-rich domains in the septal regions and at the poles of the Bacillus subtilis membrane (F. Kawai, M. Shoda, R. Harashima, Y. Sadaie, H. Hara, and K. Matsumoto, J. Bacteriol. 186:1475-1483, 2004). This finding prompted us to examine the localization of another phospholipid, phosphatidylethanolamine (PE), with the cyclic peptide probe, Ro09-0198 (Ro), that binds specifically to PE. Treatment with biotinylated Ro followed by tetramethyl rhodamine-conjugated streptavidin revealed that PE is localized in the septal membranes of vegetative cells and in the membranes of the polar septum and the engulfment membranes of sporulating cells. When the mutant cells of the strains SDB01 (psd1::neo) and SDB02 (pssA10::spc), which both lack PE, were examined under the same conditions, no fluorescence was observed. The localization of the fluorescence thus evidently reflected the localization of PE-rich domains in the septal membranes. Similar PE-rich domains were observed in the septal regions of the cells of many Bacillus species. In Escherichia coli cells, however, no PE-rich domains were found. Green fluorescent protein fusions to the enzymes that catalyze the committed steps in PE synthesis, phosphatidylserine synthase, and in CL synthesis, CL synthase and phosphatidylglycerophosphate synthase, were localized mainly in the septal membranes in B. subtilis cells. The majority of the lipid synthases were also localized in the septal membranes; this includes 1-acyl-glycerol-3-phosphate acyltransferase, CDP-diacylglycerol synthase, phosphatidylserine decarboxylase, diacylglycerol kinase, glucolipid synthase, and lysylphosphatidylglycerol synthase. These results suggest that phospholipids are produced mostly in the septal membranes and that CL and PE are kept from diffusing out to lateral ones.

scholarly journals Cloning of genes involved in membrane lipid synthesis. Effects of amplification of phosphatidylserine synthase in Escherichia coli.

1981 ◽  
Vol 256 (5) ◽  
pp. 2219-2225
Author(s):  
A. Ohta ◽  
K. Waggoner ◽  
K. Louie ◽  
W. Dowhan
Keyword(s):  
Escherichia Coli ◽  
Lipid Synthesis ◽  
Membrane Lipid ◽  
Phosphatidylserine Synthase
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