CD8+ CD122+regulatory T cells contain clonally expanded cells with identical CDR3 sequences of the T-cell receptor β-chain

AbstractTargeting T cell receptor β-chain constant region 1 (TRBC1) CAR-T could specifically kill TRBC1+ T-cell malignancies. However, over-expressed CARs on anti-TRBC1 CAR transduced TRBC1+ T cells (CAR-C1) bound to autologous TRBC1, masking TRBC1 from identification by other anti-TRBC1 CAR-T, and moreover only the remaining unoccupied CARs recognized TRBC1+ cells, considerably reducing therapeutic potency of CAR-C1. In addition, co-culture of anti-TRBC1 CAR-T and TRBC1+ cells could promote exhaustion and terminal differentiation of CAR-T. These findings provide a rationale for pre-depleting TRBC1+ T cells before anti-TRBC1 CAR-T manufacturing.

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Diversity of T cell receptor α and β chain genes expressed by human T cells specific for similar myelin basic protein peptide/major histocompatibility complexes

European Journal of Immunology ◽

10.1002/eji.1830220319 ◽

1992 ◽

Vol 22 (3) ◽

pp. 753-758 ◽

Cited By ~ 56

Author(s):

Gerhard Giegerich ◽

Martin Pette ◽

Edgar Meinl ◽

Jörg T. Epplen ◽

Hartmut Wekerle ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Myelin Basic Protein ◽

T Cell Receptor ◽

Basic Protein ◽

Cell Receptor ◽

Major Histocompatibility ◽

Human T Cells ◽

Major Histocompatibility Complexes ◽

Β Chain

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Wasp venom immunotherapy expands a subpopulation of CD4+CD25+ forkhead box protein 3–positive regulatory T cells expressing the T-cell receptor Vβ2 and Vβ5.1 chains

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2012.05.010 ◽

2012 ◽

Vol 130 (4) ◽

pp. 994-996.e3 ◽

Cited By ~ 5

Author(s):

Andreas Kerstan ◽

Niklas Beyersdorf ◽

Johanna Stoevesandt ◽

Axel Trautmann

Keyword(s):

T Cells ◽

T Cell ◽

Regulatory T Cells ◽

T Cell Receptor ◽

Cell Receptor ◽

Venom Immunotherapy ◽

Wasp Venom ◽

Forkhead Box

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Highly Biased CDR3 Usage in Restricted Sets of β Chain Variable Regions During Viral Superantigen 9 Response

Journal of Experimental Medicine ◽

10.1084/jem.187.2.253 ◽

1998 ◽

Vol 187 (2) ◽

pp. 253-258 ◽

Cited By ~ 14

Author(s):

Cristina Ciurli ◽

David N. Posnett ◽

Rafick-Pierre Sékaly ◽

François Denis

Keyword(s):

T Cells ◽

T Cell ◽

T Cell Receptor ◽

Hypervariable Region ◽

Cell Receptor ◽

Variable Regions ◽

Cdr3 Region ◽

Tumor Virus ◽

The One ◽

Β Chain

Superantigens encoded by the mouse mammary tumor virus can stimulate a large proportion of T cells through interaction with germline-encoded regions of the T cell receptor β chain like the hypervariable region 4 (HV4) loop. However, several lines of evidence suggest that somatically generated determinants in the CDR3 region might influence superantigen responses. We stimulated T cells from donors differing at the BV6S7 allele with vSAG9 to assess the nature and structure of the T cell receptor in amplified T cells and to evaluate the contribution of non-HV4 elements in vSAG recognition. This report demonstrates that vSAG9 stimulation caused the expansion of TCR BV6-expressing T cells, although to varying degrees depending on the BV6 subfamily. The BV6S7 subfamily was preferentially expanded in all donors, but in donors homozygous for the BV6S7*2 allele, a significant number of BV6S5 T cells were amplified and showed a highly biased β chain junctional region (BJ) and CDR3 usage. As CDR3 regions are involved in major histocompatibility complex (MHC)–peptide interaction, such a selection is highly suggestive of an intimate MHC–TCR interaction and would imply that the topology of the MHC-vSAG-TCR complex is similar to the one occurring during conventional antigen recognition.

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