Demonstration of western equine encephalitis virus in mouse brain by immunofluorescence
Keyword(s):
Brains of mice infected with western equine encephalitis (WEE) virus were fixed in Carnoy's fluid at 4 °C and transferred through chloroform to paraffin. The procedure gave excellent preservation of structural architecture and viral antigen. The latter was demonstrated in sections by the indirect fluorescent antibody technique in which Evans blue was used as a counterstain. The technique proved to be rapid, simple, and reliable for the identification of WEE virus in mouse brain.
1974 ◽
Vol 18
(5)
◽
pp. 411-413
◽
Keyword(s):
1959 ◽
Vol 3
(1)
◽
pp. 71-83
◽
1989 ◽
Vol 1
(2)
◽
pp. 105-109
◽
2000 ◽
Vol 41
(4-5)
◽
pp. 301-308
◽
1965 ◽
Vol 13
(3)
◽
pp. 155-160
◽
Keyword(s):