scholarly journals Phylogenetic Analysis of the Small Hydrophobic (SH) Gene of Mumps Virus in Korea: Identification of a New Genotype

2000 ◽  
Vol 44 (3) ◽  
pp. 173-177 ◽  
Author(s):  
Sang Hyun Kim ◽  
Ki-joon Song ◽  
Young Kyoo Shin ◽  
Jee Hee Kim ◽  
Soon Mi Choi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Mumps Virus ◽  
New Genotype ◽  
Small Hydrophobic

scholarly journals Characterization of two decades of temporal co-circulation of four mumps virus genotypes in Denmark: identification of a new genotype

2001 ◽  
Vol 82 (11) ◽  
pp. 2675-2680 ◽  
Author(s):  
Tesfaldet Tecle ◽  
Blenda Böttiger ◽  
Claes Örvell ◽  
Bo Johansson
Keyword(s):  
Protein Gene ◽  
Mumps Virus ◽  
Amino Acid Sequences ◽  
Nucleotide Sequencing ◽  
Similar Distribution ◽  
Serum Samples ◽  
New Genotype ◽  
Virus Genotypes ◽  
Small Hydrophobic

Twenty-nine Danish virus isolates and 14 serum samples from patients with mumps were genotyped by nucleotide sequencing of the small hydrophobic (SH) protein gene and the deduced 57 amino acid sequences were aligned with sequences of mumps virus strains published previously. Four neurovirulent genotypes of the SH protein gene, genotypes C, D, H and a new genotype, designated J, were found. There was a dynamic fluctuation of the different genotypes over the two decade period of time. Genotype J was found from 1981 to 1988; genotypes C and H exhibited a similar distribution in time. Genotype D was found between 1979 and 1982, it then disappeared and reappeared again in 1996. From 1996 onwards, genotype D was found to be the predominant genotype, which is in contrast to the situation seen in the neighbouring country of Sweden, where, since 1985, only genotype A has been found.

Phylogenetic analysis of clinical mumps virus isolates from vaccinated and non-vaccinated patients with mumps during an outbreak, Switzerland 1998-2000

2004 ◽  
Vol 73 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Silvia Utz ◽  
Jean-Luc Richard ◽  
Selja Capaul ◽  
Hans C. Matter ◽  
Meri Gorgievski Hrisoho ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Mumps Virus ◽  
Virus Isolates

scholarly journals Discrimination of Mumps Virus Small Hydrophobic Gene Deletion Effects from Gene Translation Effects on Virus Virulence

2011 ◽  
Vol 85 (12) ◽  
pp. 6082-6085 ◽  
Author(s):  
T. Malik ◽  
C. W. Shegogue ◽  
K. Werner ◽  
L. Ngo ◽  
C. Sauder ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Mumps Virus ◽  
Small Hydrophobic

scholarly journals Mumps virus small hydrophobic protein targets ataxin-1 ubiquitin-like interacting protein (ubiquilin 4)

2010 ◽  
Vol 91 (11) ◽  
pp. 2773-2781 ◽  
Author(s):  
M. Woznik ◽  
C. Rodner ◽  
K. Lemon ◽  
B. Rima ◽  
A. Mankertz ◽  
...  
Keyword(s):  
Mumps Virus ◽  
Interacting Protein ◽  
Protein Targets ◽  
Hydrophobic Protein ◽  
Small Hydrophobic Protein ◽  
Small Hydrophobic

scholarly journals The F Gene of Rodent Brain-Adapted Mumps Virus Is a Major Determinant of Neurovirulence

2007 ◽  
Vol 81 (15) ◽  
pp. 8293-8302 ◽  
Author(s):  
Ken Lemon ◽  
Bertus K. Rima ◽  
Stephen McQuaid ◽  
Ingrid V. Allen ◽  
W. Paul Duprex
Keyword(s):  
Reverse Genetics ◽  
Vero Cells ◽  
Mumps Virus ◽  
Recombinant Viruses ◽  
F Gene ◽  
Rodent Brain ◽  
Molecular Determinants ◽  
The Matrix ◽  
Hn Gene ◽  
Small Hydrophobic

ABSTRACT Prior to the introduction of live-attenuated vaccines, mumps virus (MuV) was the leading cause of virus-induced meningitis. Although vaccination has been effective at controlling the disease, the use of insufficiently attenuated strains has been associated with high rates of aseptic meningitis in vaccinees. The molecular basis of MuV attenuation is poorly understood, and no reliable molecular markers of virulence have been identified. In this study, reverse genetics has been used to identify molecular determinants of MuV neuropathogenesis. Recombinant viruses, containing the envelope-associated genes from the Kilham (MuVKH) rodent brain-adapted strain of MuV, were generated in the Jeryl Lynn 5 (MuVJL5) vaccine strain background. The syncytium phenotypes of the recombinant viruses on Vero cells differed depending on the source of the fusion (F) and hemagglutinin-neuraminidase (HN) glycoproteins, with heterologous combinations showing either an increase or a decrease in the level of cell fusion compared to that of the homologous parental combinations. This was confirmed by transiently cotransfecting eukaryotic F and HN glycoprotein expression constructs. A Lewis rat model that discriminates between neurovirulent and nonneurovirulent MuV strains based on the extent of hydrocephalus induced in the rat brain after intracerebral inoculation was used to assess the phenotype of the recombinant viruses. Expression of the matrix (M), small hydrophobic (SH), or HN gene in isolation did not confer a neurovirulent phenotype. Expression of the F gene of the neurovirulent strain alone was sufficient to induce significant levels of hydrocephalus. Coexpression of the homologous HN gene led to a marginal increase in the level of hydrocephalus.

scholarly journals Characterization of three co-circulating genotypes of the small hydrophobic protein gene of mumps virus.

1998 ◽  
Vol 79 (12) ◽  
pp. 2929-2937 ◽  
Author(s):  
T Tecle ◽  
B Johansson ◽  
C Orvell ◽  
M Forsgren ◽  
A Jejcic
Keyword(s):  
Protein Gene ◽  
Mumps Virus ◽  
Hydrophobic Protein ◽  
Small Hydrophobic Protein ◽  
Small Hydrophobic

scholarly journals Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory

2016 ◽  
Vol 31 (1) ◽  
pp. 29-31
Author(s):  
Marieke Brauer ◽  
Marianne Wolfaardt ◽  
Lynne M. Webber ◽  
Maureen B. Taylor
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Reverse Transcription ◽  
Molecular Detection ◽  
Mumps Virus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.

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