Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory
2016 ◽
Vol 31
(1)
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pp. 29-31
Keyword(s):
Rt Pcr
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The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.
1996 ◽
Vol 42
(1-4)
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pp. 13-17
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1999 ◽
Vol 32
(5)
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pp. 423-430
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