scholarly journals Fluconazole-Resistant PathogensCandida inconspicuaandC. norvegensis: DNA Sequence Diversity of the rRNA Intergenic Spacer Region, Antifungal Drug Susceptibility, and Extracellular Enzyme Production

2004 ◽  
Vol 48 (10) ◽  
pp. 761-766 ◽  
Author(s):  
Takashi Sugita ◽  
Kanji Takeo ◽  
Misako Ohkusu ◽  
Eric Virtudazo ◽  
Masako Takashima ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Enzyme Production ◽  
Intergenic Spacer ◽  
Extracellular Enzyme ◽  
Drug Susceptibility ◽  
Sequence Diversity ◽  
Antifungal Drug ◽  
Intergenic Spacer Region ◽  
Fluconazole Resistant

scholarly journals The intergenic spacer region of the ribosomal RNA gene of Penicillium marneffei shows almost no DNA sequence diversity

2010 ◽  
Vol 54 (11) ◽  
pp. 714-716 ◽  
Author(s):  
Nanthawan Mekha ◽  
Takashi Sugita ◽  
Koichi Makimura ◽  
Natteewan Poonwan ◽  
Pathom Sawanpanyalert ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Ribosomal Rna ◽  
Intergenic Spacer ◽  
Sequence Diversity ◽  
Penicillium Marneffei ◽  
Ribosomal Rna Gene ◽  
Intergenic Spacer Region

scholarly journals Differentiation of Moraxella Bovoculi sp. nov. from other Coccoid Moraxellae by the Use of Polymerase Chain Reaction and Restriction Endonuclease Analysis of Amplified DNA

2007 ◽  
Vol 19 (5) ◽  
pp. 532-534 ◽  
Author(s):  
John A. Angelos ◽  
Louise M. Ball
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequence ◽  
Restriction Analysis ◽  
Intergenic Spacer ◽  
Restriction Enzyme Digestion ◽  
Chain Reaction ◽  
Gram Negative ◽  
Intergenic Spacer Region ◽  
Polymerase Chain ◽  
Moraxella Bovoculi

Moraxella oris was historically the only coccoid Moraxella identified in cultures of ocular fluid from cattle with infectious bovine keratoconjunctivitis (IBK) and could be morphologically and biochemically differentiated from Moraxella bovis. Moraxella bovoculi sp. nov. is a recently characterized Moraxella isolated from ulcerated eyes of calves with IBK in northern California in 2002. Like Moraxella ovis, M. bovoculi sp. nov. is a gram-negative coccus/diplococcus. All 18 original isolates of M. bovoculi sp. nov. possessed phenylalanine deaminase (PADase) activity and could therefore be differentiated from M. ovis and M. bovis. During the characterization of 44 additional isolates of hemolytic gram-negative cocci that were cultured from ulcerated eyes of IBK-affected calves, 2 PADase-negative isolates were identified that could not be differentiated biochemically from M. ovis; however, the DNA sequence of the 16S-23S intergenic spacer region (ISR) of the isolates matched the 16S-23S ISR DNA sequence of M. bovoculi sp. nov. To facilitate the identification of PADase-negative moraxellae, a polymerase chain reaction (PCR) coupled with restriction enzyme digestion analysis of amplified DNA was developed. Amplification of the 16S-23S ISR followed by AfaI digestion of amplified DNA could differentiate M. bovoculi sp. nov. from M. ovis and other moraxellae. The DNA sequence analysis of the amplified 16S-23S ISR from the 42 PADase-positive isolates of hemolytic gram-negative cocci indicated that all were M. bovoculi sp. nov. and all possessed an AfaI site. A PCR coupled with restriction analysis of amplified DNA can aid in identifying M. bovoculi sp. nov.

scholarly journals Absence of DNA sequence diversity of the intergenic spacer 1 region inMalassezia nanaisolates from cats

2010 ◽  
Vol 48 (2) ◽  
pp. 427-429 ◽  
Author(s):  
Filippo de Bellis ◽  
Gemma Castellá ◽  
F. Javier Cabañes ◽  
Ross Bond
Keyword(s):  
Dna Sequence ◽  
Intergenic Spacer ◽  
Sequence Diversity

scholarly journals Armillaria Root-Rot Pathogens: Species Boundaries and Global Distribution

Pathogens ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 83 ◽  
Author(s):  
Martin Coetzee ◽  
Brenda Wingfield ◽  
Michael Wingfield
Keyword(s):  
Dna Sequence ◽  
Root Rot ◽  
Sequence Data ◽  
Current Knowledge ◽  
Single Gene ◽  
Intergenic Spacer ◽  
Species Boundaries ◽  
Intergenic Spacer Region ◽  
Biogeographic History ◽  
Armillaria Root Rot

This review considers current knowledge surrounding species boundaries of the Armillaria root-rot pathogens and their distribution. In addition, a phylogenetic tree using translation elongation factor subunit 1-alpha (tef-1α) from isolates across the globe are used to present a global phylogenetic framework for the genus. Defining species boundaries based on DNA sequence-inferred phylogenies has been a central focus of contemporary mycology. The results of such studies have in many cases resolved the biogeographic history of species, mechanisms involved in dispersal, the taxonomy of species and how certain phenotypic characteristics have evolved throughout lineage diversification. Such advances have also occurred in the case of Armillaria spp. that include important causal agents of tree root rots. This commenced with the first phylogeny for Armillaria that was based on IGS-1 (intergenic spacer region one) DNA sequence data, published in 1992. Since then phylogenies were produced using alternative loci, either as single gene phylogenies or based on concatenated data. Collectively these phylogenies revealed species clusters in Armillaria linked to their geographic distributions and importantly species complexes that warrant further research.

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