Simple determination of nitrate in soils by second-derivative spectroscopy

The present study describes employing second derivative spectrophotometry for simultaneous determination of atenolol and amlodipine in pure form and in commercial formulations. The method is simple, accurate, precise and economic. Zero crossing point technique was used for analysis of the drugs in the combined formulation. The method was found to be linear in the concentration range 5.0-50.0µg/ml of atenolol at 251nm and 5.0-45.0µg/ml of amlodipine at 264nm. The proposed method was successfully applied to determine atenolol and amlodipine in combined dosage as well as in a separate dosage. The obtained results were in good agreement with standard method.

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Simultaneous determination of tyrosine and tryptophan residues in proteins by second-derivative spectroscopy

Analytical Biochemistry ◽

10.1016/0003-2697(82)90512-7 ◽

1982 ◽

Vol 126 (2) ◽

pp. 251-257 ◽

Cited By ~ 76

Author(s):

Luigi Servillo ◽

Giovanni Colonna ◽

Ciro Balestrieri ◽

Raffaele Ragone ◽

Gaetano Irace

Keyword(s):

Simultaneous Determination ◽

Second Derivative ◽

Derivative Spectroscopy ◽

Tryptophan Residues ◽

Second Derivative Spectroscopy

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Environmental Method to Determine Dopamine and Ascorbic Acid Simultaneously via Derivative Spectrophotometry

Journal of Spectroscopy ◽

10.1155/2013/260376 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Ahmed M. El-Zohry ◽

E. Y. Hashem

Keyword(s):

Ascorbic Acid ◽

Simultaneous Determination ◽

Absorption Spectra ◽

High Precision ◽

Ph Value ◽

Hazardous Materials ◽

Second Derivative ◽

Derivative Spectroscopy ◽

Second Derivative Spectroscopy

Many various methods were applied to determine dopamine and ascorbic acid simultaneously using hazardous materials and complex procedures. Derivative absorption spectra can give safely and five sensitive derivative equations that are used for the simultaneous determination of dopamine and ascorbic acid in the UV region, using first and second derivative spectroscopy with high precision at pH value of 9.2. Dopamine and ascorbic acid can be detected in the ranges of 0.375–9.45 mg L−1and 0.352–5.28 mg L−1, respectively. These obtained methods could be used to determine both reagents in real and synthesized samples.

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Carboxyhemoglobin determination by second-derivative spectroscopy.

Clinical Chemistry ◽

10.1093/clinchem/31.2.279 ◽

1985 ◽

Vol 31 (2) ◽

pp. 279-281 ◽

Cited By ~ 17

Author(s):

J Parks ◽

H G Worth

Keyword(s):

Spectrum Analysis ◽

Spectral Shift ◽

Zero Order ◽

Reduced Form ◽

Second Derivative ◽

Derivative Spectrum ◽

Derivative Spectroscopy ◽

Set Up ◽

Second Derivative Spectroscopy

Abstract In this procedure hemoglobin is converted to its reduced form and the magnitude of the zero-order spectral shift of the reduced hemoglobin peak at 430 nm to the carboxyhemoglobin peak at 418 nm is determined by second-derivative spectrum analysis. The method is simple, straightforward to set up, and rapid. A result may be obtained within 15 min of receiving the sample. It is sufficiently sensitive to differentiate carboxyhemoglobin concentration in the blood of smokers and nonsmokers.

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