Simultaneous Determination of Atenolol and Amlodipine Using Second Derivative Spectroscopy

The present study describes employing second derivative spectrophotometry for simultaneous determination of atenolol and amlodipine in pure form and in commercial formulations. The method is simple, accurate, precise and economic. Zero crossing point technique was used for analysis of the drugs in the combined formulation. The method was found to be linear in the concentration range 5.0-50.0µg/ml of atenolol at 251nm and 5.0-45.0µg/ml of amlodipine at 264nm. The proposed method was successfully applied to determine atenolol and amlodipine in combined dosage as well as in a separate dosage. The obtained results were in good agreement with standard method.

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Simultaneous Determination of Metoprolol-Hydrochlorothiazide and Propranolol-Hydrochlorothiazide in Combined Formulations by Derivative Spectroscopy

Journal of AOAC International ◽

10.1093/jaoac/80.2.325 ◽

1997 ◽

Vol 80 (2) ◽

pp. 325-330 ◽

Cited By ~ 14

Author(s):

Challapalli V N Prasad ◽

Vipin Bharadwaj ◽

Vidya Narsimhan ◽

Rama T Chowdhary ◽

Pyare Parimoo

Keyword(s):

Simultaneous Determination ◽

Second Derivative ◽

Zero Crossing ◽

Derivative Spectra ◽

Derivative Spectroscopy ◽

Naoh Solution ◽

Compensation Technique ◽

Second Derivative Spectra ◽

Crossing Point

Abstract A derivative spectrophotometric procedure was established for simultaneous determination of propranolol HCI (PP) with hydrochlorothiazide (HTZ) and metoprolol tartrate (MTP) with HTZ in tablet preparations. The method uses first- and second-derivative spectra of tablet extract in 0.01 N NaOH solution. Ratios of analyte concentrations in the mixture were determined by the compensation technique. The zero-crossing point (ZCP) was also used to estimate the amounts of PP and HTZ in the formulations, and results were compared with those from the compensation technique. The results were found to be precise and free from interferences.

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A Rapid, Simultaneous Determination of Paraquat and Diquat in Serum and Urine Using Second-Derivative Spectroscopy

Journal of Analytical Toxicology ◽

10.1093/jat/16.4.214 ◽

1992 ◽

Vol 16 (4) ◽

pp. 214-216 ◽

Cited By ~ 31

Author(s):

Chiaki Fuke ◽

Kiyoshi Ameno ◽

Setsuko Ameno ◽

Takahiro Kirlu ◽

Toyohiko Shinohara ◽

...

Keyword(s):

Simultaneous Determination ◽

Second Derivative ◽

Derivative Spectroscopy ◽

Second Derivative Spectroscopy ◽

Serum And Urine

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Simultaneous Determination of Estradiol and Medroxyprogesterone Acetate in Pharmaceutical Formulations by Second-Derivative Spectrophotometry

Journal of AOAC International ◽

10.1093/jaoac/85.4.883 ◽

2002 ◽

Vol 85 (4) ◽

pp. 883-888 ◽

Cited By ~ 6

Author(s):

M Inés Toral ◽

César Soto ◽

Pablo Richter ◽

Ana E Tapai

Keyword(s):

Simultaneous Determination ◽

Medroxyprogesterone Acetate ◽

Signal To Noise Ratio ◽

Pharmaceutical Formulations ◽

Derivative Spectrophotometry ◽

Fast Method ◽

Second Derivative ◽

Zero Crossing ◽

Relative Standard

Abstract This paper reports a simple and fast method for the simultaneous determination of estradiol (ED) and medroxyprogesterone acetate (MP) in pharmaceutical formulations by second-derivative spectrophotometry. Methanol was used to extract the drugs from formulations, and subsequently the extracts were evaluated directly by derivative spectrophotometry. The drugs were determined simultaneously by using the graphic method at 297.4 nm for ED and the zero-crossing method at 273.4 nm for MP. If both compounds are present together in a sample, it is possible to quantitate one in the presence of the other. The best signal-to-noise ratio was found when the second derivative of the spectrum was used. The linear ranges for determination of the drugs were 4.7 × 10−6 to 1.6 × 10−4 and 7.2 × 10−6 to 2.0 × 10−4 mol/L for ED and MP, respectively. The ingredients commonly found in commercial pharmaceutical formulations do not interfere with the determination. Chemical and spectral variables were optimized for the determination of both analytes. Good levels of repeatability (relative standard deviation), 1.4 and 1.9%, were obtained for ED and MP, respectively. The proposed method was applied to the determination of these drugs in pharmaceutical formulations.

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Zero-Crossing Second Derivative Spectrophotometry for Simultaneous Determination of Palladium and Nickel Using 1-(2-Pyridylazo)-2-Naphthol in Mixed Micellar Solutions

Annali di Chimica ◽

10.1002/adic.200490072 ◽

2004 ◽

Vol 94 (78) ◽

pp. 591-599 ◽

Cited By ~ 2

Author(s):

Habibollah Eskandari

Keyword(s):

Simultaneous Determination ◽

Derivative Spectrophotometry ◽

Second Derivative ◽

Micellar Solutions ◽

Zero Crossing

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Simultaneous determination of tyrosine and tryptophan residues in proteins by second-derivative spectroscopy

Analytical Biochemistry ◽

10.1016/0003-2697(82)90512-7 ◽

1982 ◽

Vol 126 (2) ◽

pp. 251-257 ◽

Cited By ~ 76

Author(s):

Luigi Servillo ◽

Giovanni Colonna ◽

Ciro Balestrieri ◽

Raffaele Ragone ◽

Gaetano Irace

Keyword(s):

Simultaneous Determination ◽

Second Derivative ◽

Derivative Spectroscopy ◽

Tryptophan Residues ◽

Second Derivative Spectroscopy

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Environmental Method to Determine Dopamine and Ascorbic Acid Simultaneously via Derivative Spectrophotometry

Journal of Spectroscopy ◽

10.1155/2013/260376 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Ahmed M. El-Zohry ◽

E. Y. Hashem

Keyword(s):

Ascorbic Acid ◽

Simultaneous Determination ◽

Absorption Spectra ◽

High Precision ◽

Ph Value ◽

Hazardous Materials ◽

Second Derivative ◽

Derivative Spectroscopy ◽

Second Derivative Spectroscopy

Many various methods were applied to determine dopamine and ascorbic acid simultaneously using hazardous materials and complex procedures. Derivative absorption spectra can give safely and five sensitive derivative equations that are used for the simultaneous determination of dopamine and ascorbic acid in the UV region, using first and second derivative spectroscopy with high precision at pH value of 9.2. Dopamine and ascorbic acid can be detected in the ranges of 0.375–9.45 mg L−1and 0.352–5.28 mg L−1, respectively. These obtained methods could be used to determine both reagents in real and synthesized samples.

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Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2014.7.1.6 ◽

2014 ◽

Vol 7 (1) ◽

pp. 2350-2355

Author(s):

Pratik S Mehta ◽

Pratik R. Patel ◽

Rajesh R Parmar ◽

M M K Modasiya ◽

Dushyant A Shah

Keyword(s):

Spectroscopic Method ◽

Spectral Interference ◽

Synthetic Mixture ◽

Simultaneous Estimation ◽

Zero Crossing ◽

First Order ◽

Derivative Spectroscopy ◽

Development And Validation ◽

Crossing Point

A novel, simple, accurate, sensitive, precise and economical derivative spectroscopic method was developed and validated for the determination of cefadroxil and probenecid in synthetic mixture. First order derivative spectroscopy method was adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 4-36 μg/ml for cefadroxil and of 5-25 μg/ml of probenecid. The zero crossing point for cefadroxil and probenecid was 260 nm and 237.8 nm respectively in 0.1N HCl. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found.

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Determination of serum methemalbumin by second-derivative spectroscopy

Clinica Chimica Acta ◽

10.1016/0009-8981(82)90120-6 ◽

1982 ◽

Vol 123 (1-2) ◽

pp. 121-126 ◽

Cited By ~ 7

Author(s):

Alain Bertrand ◽

Claude Cox ◽

Pierre Foucart ◽

Jean Buret

Keyword(s):

Second Derivative ◽

Derivative Spectroscopy ◽

Second Derivative Spectroscopy

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Application of Derivative Spectrophotometry for Simultaneous Determination of Quinapril and Hydrochlorothiazide in the Combination Tablets

Journal of AOAC International ◽

10.1093/jaoac/87.4.847 ◽

2004 ◽

Vol 87 (4) ◽

pp. 847-851 ◽

Cited By ~ 2

Author(s):

Dorota Kowalczuk ◽

Hanna Hopkała

Keyword(s):

Standard Deviation ◽

Simultaneous Determination ◽

Relative Standard Deviation ◽

Spectrophotometric Method ◽

Derivative Spectrophotometry ◽

Order Derivative ◽

Zero Crossing ◽

Relative Standard ◽

Separation Of Components

Abstract A new second-order-derivative spectrophotometric method using zero-crossing technique measures quinapril (QUI) and hydrochlorothiazide (HYD) in 2-component mixtures. The procedure does not require prior separation of components from the sample. QUI was determined at a wavelength of 211.6 nm (zero-crossing wavelength point of HYD). Similarly, HYD was measured at 270.8 nm (zero-crossing wavelength point of QUI). Calibration graphs were constructed over the concentration range of 4.0 to 24.0 μ/mL for QUI and 2.5 to 15.0 μg/mL for HYD. Detection and quantitation limits were 0.85 and 2.5 μg/mL for QUI and 0.12 and 0.4 μg/mL for HYD, respectively. The accuracy (recovery 100.5–102%), precision (relative standard deviation less than 3.5% for QUI and 1.5% for HYD), selectivity, and sensitivity of the elaborated methods were satisfactory. The proposed method was applied successfully for the determination of both drugs in QUI-HYD tablets.

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