Chitosan/double-stranded RNA nanoparticle-mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambiae)

2010 ◽  
Vol 19 (5) ◽  
pp. 683-693 ◽  
Author(s):  
X. Zhang ◽  
J. Zhang ◽  
K. Y. Zhu
Keyword(s):  
Rna Interference ◽  
Anopheles Gambiae ◽  
Chitin Synthase ◽  
Larval Feeding ◽  
Malaria Mosquito ◽  
Double Stranded Rna ◽  
Mosquito Anopheles Gambiae

scholarly journals RNAi by Soaking Aedes aegypti Pupae in dsRNA

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 634
Author(s):  
Fiza Arshad ◽  
Arvind Sharma ◽  
Charleen Lu ◽  
Monika Gulia-Nuss
Keyword(s):  
Functional Analysis ◽  
Rna Interference ◽  
Aedes Aegypti ◽  
Simple Procedure ◽  
Standard Technique ◽  
Malaria Mosquito ◽  
Double Stranded Rna ◽  
Carrier Molecule ◽  
Mosquito Anopheles Gambiae ◽  
Osmotic Challenge

RNA-interference (RNAi) is a standard technique for functional genomics in adult mosquitoes. However, RNAi in immature, aquatic mosquito stages has been challenging. Several studies have shown successful larval RNAi, usually in combination with a carrier molecule. Except for one study in malaria mosquito, Anopheles gambiae, none of the previous studies has explored RNAi in mosquito pupae. Even in the study that used RNAi in pupae, double stranded RNA (dsRNA) was introduced by microinjection. Here, we describe a successful method by soaking pupae in water containing dsRNA without any carrier or osmotic challenge. The knockdown persisted into adulthood. We expect that this simple procedure will be useful in the functional analysis of genes that highly express in pupae or newly emerged adults.

scholarly journals Low-resolution genome map of the malaria mosquito Anopheles gambiae.

1991 ◽  
Vol 88 (24) ◽  
pp. 11187-11191 ◽  
Author(s):  
L. B. Zheng ◽  
R. D. Saunders ◽  
D. Fortini ◽  
A. della Torre ◽  
M. Coluzzi ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito ◽  
Low Resolution ◽  
Genome Map ◽  
Mosquito Anopheles Gambiae

scholarly journals Odorant reception in the malaria mosquito Anopheles gambiae

Nature ◽  
2010 ◽  
Vol 464 (7285) ◽  
pp. 66-71 ◽  
Author(s):  
Allison F. Carey ◽  
Guirong Wang ◽  
Chih-Ying Su ◽  
Laurence J. Zwiebel ◽  
John R. Carlson
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito ◽  
Mosquito Anopheles Gambiae

scholarly journals Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae

2017 ◽  
Vol 13 (1) ◽  
pp. e1006113 ◽  
Author(s):  
Gloria Volohonsky ◽  
Ann-Katrin Hopp ◽  
Mélanie Saenger ◽  
Julien Soichot ◽  
Heidi Scholze ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito ◽  
Transgenic Expression ◽  
Mosquito Anopheles Gambiae
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