Use of Bean Sprout Enterobacteriaceae Isolates as Biological Control Agents of Pseudomonas fluorescens

2004 ◽  
Vol 69 (1) ◽  
pp. FMS17-FMS22 ◽  
Author(s):  
K. ENOMOTO
Keyword(s):  
Biological Control ◽  
Pseudomonas Fluorescens ◽  
Biological Control Agents ◽  
Bean Sprout

scholarly journals Control of Fire Blight by Pseudomonas fluorescens A506 and Pantoea vagans C9-1 Applied as Single Strains and Mixed Inocula

2010 ◽  
Vol 100 (12) ◽  
pp. 1330-1339 ◽  
Author(s):  
V. O. Stockwell ◽  
K. B. Johnson ◽  
D. Sugar ◽  
J. E. Loper
Keyword(s):  
Biological Control ◽  
Pseudomonas Fluorescens ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Field Trials ◽  
Disease Suppression ◽  
Biological Control Agents ◽  
Population Sizes ◽  
Mixed Inocula ◽  
Pantoea Vagans

The biological control agents Pseudomonas fluorescens A506 and Pantoea vagans C9-1 were evaluated individually and in combination for the suppression of fire blight of pear or apple in 10 field trials inoculated with the pathogen Erwinia amylovora. The formulation of pathogen inoculum applied to blossoms influenced establishment of the pathogen and the efficacy of biological control. Pantoea vagans C9-1 suppressed fire blight in all five trials in which the pathogen was applied as lyophilized cells but in none of the trials in which the pathogen was applied as freshly harvested cells. In contrast, Pseudomonas fluorescens A506 reduced disease significantly in only one trial. A mixture of the two strains also suppressed fire blight, but the magnitude of disease suppression over all field trials (averaging 32%) was less than that attained by C9-1 alone (42%). The two biological control agents did not antagonize one another on blossom surfaces, and application of the mixture of A506 and C9-1 to blossoms resulted in a greater proportion of flowers having detectable populations of at least one bacterial antagonist than the application of individual strains. Therefore, the mixture of A506 and C9-1 provided less disease control than expected based upon the epiphytic population sizes of the antagonists on blossom surfaces. We speculate that the biocontrol mixture was less effective than anticipated due to incompatibility between the mechanisms by which A506 and C9-1 suppress disease.

scholarly journals Biological Control to Protect Watermelon Blossoms and Seed from Infection by Acidovorax avenae subsp. citrulli

2005 ◽  
Vol 95 (4) ◽  
pp. 413-419 ◽  
Author(s):  
A. Fessehaie ◽  
R. R. Walcott
Keyword(s):  
Biological Control ◽  
Pseudomonas Fluorescens ◽  
Disease Suppression ◽  
Biological Control Agents ◽  
Positive Bacterium ◽  
Control Seed ◽  
Bacterial Fruit Blotch ◽  
Feasible Option ◽  
Phosphate Buffered Saline ◽  
Seed Infestation

The efficacy of biological control seed treatments with Pseudomonas fluorescens (A506), Acidovorax avenae subsp. avenae (AAA 99-2), and an unidentified gram-positive bacterium recovered from watermelon seed (WS-1) was evaluated for the management of bacterial fruit blotch (BFB) of watermelon. In growth chamber and greenhouse experiments, seed treated with AAA 99-2 displayed superior disease suppression, reducing BFB transmission by 96.5%. AAA 99-2, P. fluorescens A506, and Kocide also suppressed the epiphytic growth of A. avenae subsp. citrulli when applied to attached watermelon blossoms 5 h prior to inoculation. Watermelon blossom protection reduced seed infestation by A. avenae subsp. citrulli. From blossoms treated with 0.1 M phosphate buffered saline (PBS), 63% of the resulting seed lots were infested with A. avenae subsp. citrulli. In contrast, for blossoms protected with WS-1, Kocide, P. fluorescens A506, and AAA 99-2, the proportion of infested seed lots were 48.3, 21.1, 24.1, and 13.8%, respectively. The effect of blossom treatments on seed lot infestation was statistically significant (P = 0.001) but WS-1 was not significantly different from PBS. These findings suggest that blossom protection with biological control agents could be a feasible option for managing BFB.

scholarly journals Effect of prohexadionecalcium on nectar composition of pomaceous flowers and on bacterial growth

2005 ◽  
Vol 58 ◽  
pp. 106-111
Author(s):  
F. Spinelli ◽  
J.L Vanneste ◽  
G.L. Marcazzjin ◽  
A.G. Sabatini ◽  
G. Costa
Keyword(s):  
Biological Control ◽  
Pseudomonas Fluorescens ◽  
Bacterial Growth ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Pantoea Agglomerans ◽  
Sugar Concentration ◽  
Sugar Solution ◽  
Biological Control Agents ◽  
Nectar Composition

Prohexadionecalcium is an acylcyclohexanedione that reduces incidence of fire blight caused by Erwinia amylovora To determine whether prohexadionecalcium can be used in conjunction with biological control agents its effect on the secretion and composition of nectar from apple and pear flowers was evaluated Plants treated with prohexadionecalcium produced more nectar but the sugar concentration of the nectar was lower The ability of E amylovora and of two biological control agents of fire blight Pantoea agglomerans and Pseudomonas fluorescens to grow in a sugar solution corresponding to the composition of nectar from treated or untreated plants was determined in the laboratory All bacteria including E amylovora grew better in the sugar solution corresponding to the nectar from treated plants The concentration of sugar in the nontreated flowers may be too high for bacterial growth These results also suggest that prohexadionecalcium can be used alongside biological control agents for reduction of fire blight incidence

scholarly journals Colonisation of apple and pear leaves by different strains of biological control agents of fire blight

2004 ◽  
Vol 57 ◽  
pp. 49-53 ◽  
Author(s):  
J.L. Vanneste ◽  
D.A. Cornish ◽  
F. Spinelli ◽  
J. Yu
Keyword(s):  
Biological Control ◽  
New Zealand ◽  
Pseudomonas Fluorescens ◽  
Leaf Area ◽  
Fire Blight ◽  
Leaf Surface ◽  
Apple Orchards ◽  
Spray Application ◽  
Biological Control Agents ◽  
Different Strains

The ability of biological control agents of fire blight to establish and colonise the surface of apple and pear leaves was investigated Two commercially available biological control agents Pantoea agglomerans P10c and Pseudomonas fluorescens A506 and two strains of P agglomerans isolated from apple orchards in New Zealand were used All of these strains are able to reduce fire blight incidence on apple and pear flowers After spray application the percentage of leaf surface colonised by the biological control agents was measured by imprinting leaves on fresh agar plates Pseudomonas fluorescens A506 did not survive for more than two days in the field while the strains of P agglomerans survived for several days but the percentage of leaf area colonised decreased rapidly after 48 hours These strains might not have the characteristics necessary to control fire blight infections on shoots

Yellow Brazilian Pepper-tree Leaf Galler (suggested common name) Calophya latiforceps Burckhardt (Insecta: Hemiptera: Calophyidae: Calophyinae)

EDIS ◽  
2017 ◽  
Vol 2017 (6) ◽  
Author(s):  
James P. Cuda ◽  
Patricia Prade ◽  
Carey R. Minteer-Killian
Keyword(s):  
Biological Control ◽  
North America ◽  
Natural Enemies ◽  
Host Plants ◽  
Classical Biological Control ◽  
Biological Control Agents ◽  
Pepper Tree ◽  
Brazilian Pepper ◽  
Close Relatives ◽  
Tree Leaf

In the late 1970s, Brazilian peppertree, Schinus terebinthifolia Raddi (Sapindales: Anacardiaceae), was targeted for classical biological control in Florida because its invasive properties (see Host Plants) are consistent with escape from natural enemies (Williams 1954), and there are no native Schinus spp. in North America. The lack of native close relatives should minimize the risk of damage to non-target plants from introduced biological control agents (Pemberton 2000). [...]

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