A Cooling Modification to an Apparatus for Phage Typing ofStaphylococcus aureus

SummarySixty-nineStaphylococcus aureusstrains. 39 of which produced staphylococcal enterotoxin B (SEB + ) and 14 of which were associated with toxic shock (TS + ), were studied using the following markers: serotyping, phage typing, antibiotyping. ribotyping, zymotyping and pulsed-field electrophoresis typing. Analysis of the results showed that the enterotoxin B producing strains were derived from at least three clones: the first two consisted of methicillin-susceptible strains, while the third included the methicillin-resistant (MRSA) strains. TS+ strains of non-genital origin appeared to be distributed between the three clones, with no specific characters.

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IsdG and IsdI, Heme-degrading Enzymes in the Cytoplasm ofStaphylococcus aureus

Journal of Biological Chemistry ◽

10.1074/jbc.m307952200 ◽

2003 ◽

Vol 279 (1) ◽

pp. 436-443 ◽

Cited By ~ 183

Author(s):

Eric P. Skaar ◽

Andrew H. Gaspar ◽

Olaf Schneewind

Keyword(s):

Ofstaphylococcus Aureus ◽

Degrading Enzymes

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Effect of pharynx epithelial cells surface desialylation on receptor-mediated adherence ofStaphylococcus aureus

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2009.04525.x ◽

2010 ◽

Vol 108 (4) ◽

pp. 1313-1322 ◽

Cited By ~ 4

Author(s):

S. Sakarya ◽

M.B. Ertugrul ◽

T. Öztürk ◽

C. Gökbulut

Keyword(s):

Epithelial Cells ◽

Ofstaphylococcus Aureus

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Typing ofSalmonella entericaserovar Infantis isolates from 51 outbreaks in Germany between 1974 and 2009 by a novel phage-typing scheme

Epidemiology and Infection ◽

10.1017/s095026881300037x ◽

2013 ◽

Vol 142 (1) ◽

pp. 75-83 ◽

Cited By ~ 9

Author(s):

T. MILLER ◽

P. G. BRAUN ◽

K. FEHLHABER ◽

R. PRAGER ◽

Y. PFEIFER ◽

...

Keyword(s):

Broiler Chickens ◽

High Stability ◽

Animal Species ◽

Pulsed Field Gel Electrophoresis ◽

Phage Typing ◽

Typing Method ◽

Typing Scheme ◽

Strain Collection ◽

Community Outbreaks

SUMMARYWe developed a new phage-typing method and evaluated its application in combination withXbaI macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) as a useful tool for the long-term epidemiology ofSalmonella entericaserovar Infantis. In this study, we investigated 1008S.Infantis isolates recovered from humans, various animal species and food products from 1973 to 2009. The typing scheme is based on 17 typing phages, defining 61 different patterns within the strain collection. The experiments showed that phage typing is a reliable method for differentiation of outbreaks and sporadic clinical cases as well as for elucidation of chains of transmission. The combined analysis of phage typing and PFGE revealed the existence of epidemic clones with a high stability over time like PT29/XB27 which was identified in nosocomial salmonellosis, community outbreaks as well as in broiler chickens from 2002 to 2009.

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