Growth of yeasts, lactic and acetic acid bacteria in palm wine during tapping and fermentation from felled oil palm (Elaeis guineensis) in Ghana

Interspecific OxG hybrids of African palm Elaeis guineensis Jacq. and the American palm Elaeis oleifera Cortes produce high-oleic palm oil (HOPO) with low saturated fatty acid content. OxG hybrids are highly productive, grow slowly, and are resistant to bud rot disease. However, OxG hybrid pollen presents low viability and germinability, so assisted pollination is a must. Hybrids can produce parthenocarpic or seedless fruits, with the exogenous application of plant growth regulators. Thus, naphthalene acetic acid (NAA) effects on parthenocarpic fruits induction, bunch formation, and oil quality were evaluated. The OxG hybrid Coari x La Mé was used. NAA doses, frequency, number of applications, and the phenological stages for the treatments were defined. A total dose of 1200 mg L−1 NAA applied three or four times produced bunches with better fruit set, similar average bunch weight, and oil to dry mesocarp than those obtained with assisted pollination. At a semi-commercial scale, 1200 mg L−1 NAA induced bunches that consisted of 93% or more of seedless fruits. Bunch number (2208 ± 84 versus 1690 ± 129) and oil to bunch (32.2 ± 0.7 versus 25.3 ± 0.8) were higher in the NAA induced bunches than in the assisted pollination. However, the average bunch weight was lower (12.2 ± 0.4 versus 14.9 ± 0.6). NAA increased oil to bunch in 36% (8.7 ± 0.1 versus 6.4 ± 0.3). Thus, with this technology, it is plausible to reach more than 10 tons per hectare per year of HOPO. Potentially, without increasing the planted oil palm area, OxG hybrids and NAA applications could alone meet the world’s fats and oil demands.

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Screening and molecular characterization of cholesterol-lowering lactic acid bacteria isolated from African oil palm wine (Elaeis guineensis) and corn beer.

10.21203/rs.3.rs-106340/v1 ◽

2020 ◽

Author(s):

Betrand Tatsinkou Fossi ◽

Dickson Ebwelle Ekabe ◽

Laure Toukam ◽

Henri Olivier Tatsilong ◽

Anandine Gagneux-Brunon ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Serum Cholesterol ◽

Oil Palm ◽

Elaeis Guineensis ◽

Gram Positive ◽

Cholesterol Lowering ◽

Palm Wine ◽

Genotypic Identification ◽

African Oil Palm

Abstract Background: High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Probiotic supplementation improves health benefits by lowering serum cholesterol. African oil palm (Elaeis guineensis) wine and corn beer have been described as potential sources of probiotic bacteria, but their cholesterol lowering activity has not been extensively investigated. Therefore in this study, we isolated and characterized lactic acid bacteria with probiotic and cholesterol lowering properties from these sources.Methods: A total of fifty lactic acid bacteria (LAB) were isolated from palm wine and corn beer using de Man Rogosa and Sharpe (MRS) agar by pour plate method. Catalase negative and Gram positive colonies were considered as presumptive LAB. For probiotic properties and selection, acid and bile salt tolerance were evaluated based on viable colony count on MRS agar and cholesterol assimilation from culture media was performed by spectrophotometry. The data analysis was performed by One Way Analysis of Variance and p value< 0.05 was considered significant. The isolates with excellent cholesterol assimilation properties were selected and identified with species of morphology and biochemical analysis using API 50 CHL kit and genotypic identification by sequencing of 16 S rRNA gene.Results: Fifty isolates had morphological features similar to LAB. They were catalase negative and Gram positive colonies. Among the fifty isolates, five were considered as acid and bile tolerant (viable count exceeded 6logCFU/ml), with two isolates having excellent cholesterol assimilation property (>80%). The result obtained using API 50 CHL kit did not agree with the genotypic identification which was used to confirm the identity of the isolates as most closely related to Lactobacillus brevis ATCC (Accession No.NR116238) and Enterococcus faeccium NBRC (Accession No. NR113904).Conclusions: The two strains isolated from traditionally fermented drinks in Buea can be considered as potential probiotics. They can therefore be used to formulate food supplement needed to lower cholesterol in hypercholesterolemia patients.

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The microbiology of Bandji, palm wine of Borassus akeassii from Burkina Faso: identification and genotypic diversity of yeasts, lactic acid and acetic acid bacteria

Journal of Applied Microbiology ◽

10.1111/jam.12014 ◽

2012 ◽

Vol 113 (6) ◽

pp. 1428-1441 ◽

Cited By ~ 34

Author(s):

L.I.I. Ouoba ◽

C. Kando ◽

C. Parkouda ◽

H. Sawadogo-Lingani ◽

B. Diawara ◽

...

Keyword(s):

Acetic Acid ◽

Lactic Acid ◽

Burkina Faso ◽

Genotypic Diversity ◽

Acetic Acid Bacteria ◽

Palm Wine

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Ethnobotany of Elaeis guineensis Jacq. and its importance in the household economy of the Ikale and Ilaje of Ondo State, Nigeria

Ethnobotany Research and Applications ◽

10.32859/era.20.42.1-16 ◽

2020 ◽

Vol 20 ◽

Author(s):

Sakiru Morenikeji Erinoso ◽

David Olaniran Aworinde ◽

Olasimbo Anuoluwapo Teniola ◽

Samuel Omoniyi Ojo

Keyword(s):

Palm Oil ◽

Oil Palm ◽

Elaeis Guineensis ◽

Palm Kernel ◽

Palm Tree ◽

Perennial Crop ◽

Palm Wine ◽

Ondo State ◽

Elaeis Guineensis Jacq ◽

African Oil Palm

Background: African oil palm (Elaeis guineensis Jacq.), a perennial crop that originated from Tropical rainforest of West Africa, is one of the most important economic oil crops in Nigeria providing income for rural and semi-urban folks. This study described the ethnobotany of the plant and evaluated the extent to which it contributes to the livelihood strategies of the Ikale and Ilaje speaking people of Ondo State, Nigeria. Methods: Interviews were conducted in the local language and questionnaires were administered to willing respondents. Photographs of tools/machines used in the production of palm oil and allied products were taken. Drawings of the tools were generated on site. Results: The plant is the principal source of palm oil. Major processing tools of palm oil include cracker, presser, digester, and boiling trough. Other products that are derived from oil palm parts are palm kernel oil, kernel meat (for livestock feed), kernel shells (for fuel), palm wine, broom, fish trap, and traditional soap. All these products are made using indigenous methods. They feature in medicinal preparations, traditional ceremonies and are important household commodities. Conclusions: The products derived from oil palm tree serve as means of livelihood in the study communities and are local resources used in everyday activities of the people. The indigenous production methods observed further emphasize the role these products play in the economy of oil palm product makers and sellers. Sustainable tapping of palm wine should be encouraged in order to derive maximum benefits from oil palm tree. Keywords: Ethnobotany, Economic Botany, African Oil Palm, Ondo State, Nigeria

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Origin of Initial Microflora of Palm Wine from Oil Palm Trees (Elaeis guineensis)

Journal of Applied Bacteriology ◽

10.1111/j.1365-2672.1973.tb04142.x ◽

1973 ◽

Vol 36 (4) ◽

pp. 559-565 ◽

Cited By ~ 30

Author(s):

S. I. Faparusi

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Palm Wine ◽

Palm Trees

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EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v12n2.2011.p57-62 ◽

2011 ◽

Vol 12 (2) ◽

pp. 57 ◽

Cited By ~ 3

Author(s):

Sumaryono Sumaryono ◽

Imron Riyadi

Keyword(s):

Acetic Acid ◽

Oil Palm ◽

Root Formation ◽

Elaeis Guineensis ◽

Control Treatment ◽

Naphthalene Acetic Acid ◽

Seedling Production ◽

Ex Vitro ◽

Shoot Height ◽

Elaeis Guineensis Jacq

Plantlets of oil palm (Elaeis guineensis Jacq.) derived from so-matic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with aux-in during ex vitro acclimatization period to simplify the proce-dure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthalene-acetic acid (NAA) at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm) for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequen-cy to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indi-cated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diame-ter by 30% compared to control treatment, but did not influ-ence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plant-lets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.<br /><br />

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EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v12n2.2011.57-62 ◽

2011 ◽

Vol 12 (2) ◽

pp. 57 ◽

Cited By ~ 2

Author(s):

Sumaryono Sumaryono ◽

Imron Riyadi

Keyword(s):

Acetic Acid ◽

Oil Palm ◽

Root Formation ◽

Elaeis Guineensis ◽

Control Treatment ◽

Naphthalene Acetic Acid ◽

Seedling Production ◽

Ex Vitro ◽

Shoot Height ◽

Elaeis Guineensis Jacq

Plantlets of oil palm (Elaeis guineensis Jacq.) derived from so-matic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with aux-in during ex vitro acclimatization period to simplify the proce-dure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthalene-acetic acid (NAA) at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm) for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequen-cy to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indi-cated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diame-ter by 30% compared to control treatment, but did not influ-ence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plant-lets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.<br /><br />

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