The chitinase activity of oil palm (Elaeis guineensis Jacq.) roots against fungal endophytes and pathogenic Ganoderma boninense

Abstract. Hayati R, Lisnawita, Munir E, Basyuni M. 2020. Short Communication: MADS-box genes expression in seedlings of Elaeis guineensis Jacq. resistance to Ganoderma boninense. Biodiversitas 21: 5805-5810. Advances in molecular technology were allowed the molecular studies of the flowering genes to expected to be used to predict oil palm (Elaeis guineensis) fruit production. This study aims to identify the genetic structure associated with flowering genes, belonging to the MADS-box gene family of E. guineensis to Ganoderma boninense susceptible and resistance types. Different length of expression genes was identified for each population displayed the single band pattern. On the basis of UVITEC-1D methods, all populations (RA, RS, SS, and SA) detected overall expression in primer EGGLO and EGDEF. Moreover, several populations (SS, SA, RA) were not expressed in primer EGSQUA, EGAGL2, and EGAG. Based on the GENEALEX results, 5 sets of MADS-box family markers showed Na values were 2.20 to 3.00, and He values were 0.40–0.67. High polymorphism information content (PIC > 0.50) was found in Resistance Asymptomatic (RA) and Susceptible Asymptomatic (SA) populations (PIC = 0.82). The phylogenetic tree described clustering for populations based on genetic distances. The dendrogram was constructed with two cluster groups. The first cluster was described as resistance asymptomatic and symptomatic population. Furthermore, the second cluster consisted of two clustering susceptible asymptomatic and symptomatic populations, separately. Several oil palm genes resistant symptomatic and asymptomatic was found in one cluster, similarly in susceptible symptomatic and asymptomatic grouped more closely. This phenomenon is likely to be the sampling rate and the addictive nature of parent crossing derivatives.

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The Phenylpropanoid Pathway and Lignin in Defense against Ganoderma boninense Colonized Root Tissues in Oil Palm (Elaeis guineensis Jacq.)

Frontiers in Plant Science ◽

10.3389/fpls.2017.01395 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 13

Author(s):

Nisha T. Govender ◽

Maziah Mahmood ◽

Idris A. Seman ◽

Mui-Yun Wong

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Phenylpropanoid Pathway ◽

Ganoderma Boninense ◽

Elaeis Guineensis Jacq ◽

Root Tissues

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THE POTENTIAL OF ENDOPHYTIC Trichoderma FROM OIL PALM (Elaeis guineensis Jacq.) ROOTS OF NORTH SUMATRA, INDONESIA AGAINST Ganoderma boninense

Journal of Oil Palm Research ◽

10.21894/jopr.2019.0049 ◽

2019 ◽

Author(s):

DEDEK HARYADI

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Ganoderma Boninense ◽

Elaeis Guineensis Jacq ◽

North Sumatra

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AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v5i1.2767 ◽

2018 ◽

Vol 5 (1) ◽

pp. 53

Author(s):

Bedah Rupaedah ◽

Debby Viola Amanda ◽

Reni Indrayanti ◽

Nia Asiani ◽

Bambang Sukmadi ◽

...

Keyword(s):

Plant Height ◽

Elaeis Guineensis ◽

Disease Incidence ◽

Chitinase Activity ◽

Ganoderma Boninense ◽

Trichoderma Sp ◽

Three Stages ◽

Elaeis Guineensis Jacq ◽

Stenotrophomonas Rhizophila ◽

The Stability

Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninense ABSTRACTBasal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp. ABSTRAKPenyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp.

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