A system based in part on three-dimensional structural relationships is described for precisely characterizing the location of cells within secretory complexes of the adult female mouse submandibular gland. The pattern of DNA synthesis during a 90-minute pulse with 3H-thymidine was characterized based upon the above system. Seventy-eight percent of all radiolabeled nuclei were found in the intercalated duct system. One-half of these were in second-order intercalated ducts. DNA synthesis was also observed in acinar cells, granular intercalated duct cells, striated granular duct cells, and granular duct cells. Some secretory complexes contained multiple radiolabeled nuclei, with some of these nuclei in a side-by-side configuration. Approximately one-half of all secretory complexes contained radiolabeled nuclei. A second survey of the frequency of complexes containing radiolabeled nuclei was conducted following four pulses at eight-hour intervals over a 26-hour period. Only about 30% of all complexes contained radiolabeled nuclei. This reduction in the frequency of radiolabeled nuclei when compared with the single pulse suggests the possibility of individual variation. However, a more prolonged period of daily injections for nine days with 3H-thymidine resulted in all but one of the secretory complexes containing radiolabeled nuclei. This latter observation suggests that cell addition in adult submandibular glands is widespread.
Distribution of Kisspeptin Neurones in the Adult Female Mouse Brain