Repression and derepression of conjugation of plasmid R1 by wild-type and mutated finP antisense RNA

1991 ◽  
Vol 5 (1) ◽  
pp. 77-87 ◽  
Author(s):  
G. Koraimann ◽  
C. Koraimann ◽  
V. Koronakis ◽  
S. Schlager ◽  
G. Högenauer
Keyword(s):  
Antisense Rna ◽  
Wild Type ◽  
Plasmid R1

Antisense RNA in Replication Control of the INC FII Plasmid R1

1988 ◽  
Vol 7 (5-6) ◽  
pp. 559-564
Author(s):  
E. G. H. Wagner ◽  
C. Persson ◽  
M. Öhman ◽  
K. Nordstrom
Keyword(s):  
Antisense Rna ◽  
Plasmid R1 ◽  
Replication Control

scholarly journals Translational Regulation by an Intramolecular Stem-Loop Is Required for Intermolecular RNA Regulation of the par Addiction Module

2008 ◽  
Vol 190 (18) ◽  
pp. 6076-6083 ◽  
Author(s):  
Sonia Shokeen ◽  
Smita Patel ◽  
Tony J. Greenfield ◽  
Cassandra Brinkman ◽  
Keith E. Weaver
Keyword(s):  
Antisense Rna ◽  
Translational Regulation ◽  
Translational Repression ◽  
Wild Type ◽  
Rna Regulation ◽  
Stem Loop ◽  
Gram Positive Bacteria ◽  
Stability Determinant ◽  
Rna I

ABSTRACT The par stability determinant of Enterococcus faecalis plasmid pAD1 is the only antisense RNA-regulated addiction module identified to date in gram-positive bacteria. par encodes two small, convergently transcribed RNAs, designated RNA I and RNA II, that function as the toxin (Fst)-encoding and antitoxin components, respectively. Previous work showed that structures at the 5′ end of RNA I are important in regulating its translation. The work presented here reveals that a stem-loop sequestering the Fst ribosome binding site is required for translational repression but a helix sequestering the 5′ end of RNA I is not. Furthermore, disruption of the stem-loop prevented RNA II-mediated repression of Fst translation in vivo. Finally, although Fst-encoding wild-type RNA I is not toxic in Escherichia coli, mutations affecting stem-loop stability resulted in toxicity in this host, presumably due to increased translation.

scholarly journals SpoIIE Regulates Sporulation but Does Not Directly Affect Solventogenesis in Clostridium acetobutylicum ATCC 824

2005 ◽  
Vol 187 (6) ◽  
pp. 1930-1936 ◽  
Author(s):  
Miles C. Scotcher ◽  
George N. Bennett
Keyword(s):  
Gene Expression ◽  
Antisense Rna ◽  
Clostridium Acetobutylicum ◽  
Promoter Activity ◽  
Normal Growth ◽  
Wild Type ◽  
Solvent Production ◽  
Intact Copy ◽  
Clostridium Acetobutylicum Atcc 824 ◽  
Production Strain

ABSTRACT Using gene expression reporter vectors, we examined the activity of the spoIIE promoter in wild-type and spo0A-deleted strains of Clostridium acetobutylicum ATCC 824. In wild-type cells, the spoIIE promoter is active in a transient manner during late solventogenesis, but in strain SKO1, where the sporulation initiator spo0A is disrupted, no spoIIE promoter activity is detectable at any stage of growth. Strains 824(pMSpo) and 824(pASspo) were created to overexpress spoIIE and to decrease spoIIE expression via antisense RNA targeted against spoIIE, respectively. Some cultures of strains 824(pMSpo) degenerated during fermentations by losing the pSOL1 megaplasmid and hence did not produce the solvents ethanol, acetone, and butanol. The frequent degeneration event was shown to require an intact copy of spoIIE. Nondegenerate cultures of 824(pMSpo) exhibited normal growth and solvent production. Strain 824(pASspo) exhibited prolonged solventogenesis characterized by increased production of ethanol (225%), acetone (43%), and butanol (110%). Sporulation in strains harboring pASspo was significantly delayed, with sporulating cells exhibiting altered morphology. These results suggest that SpoIIE has no direct effect on the control of solventogenesis and that the changes in solvent production in spoIIE-downregulated cells are mediated by effects on the cell during sporulation.

Antisense RNA inhibition of pyruvate, orthophosphate dikinase and NADP malate dehydrogenase in the C4 plant Flaveria bidentis: analysis of plants with a mosaic phenotype

1999 ◽  
Vol 26 (6) ◽  
pp. 537 ◽  
Author(s):  
Anthony R. Ashton ◽  
Robert T. Furbank ◽  
Stephen J. Trevanion
Keyword(s):  
Transgenic Plants ◽  
Malate Dehydrogenase ◽  
Antisense Rna ◽  
Wild Type ◽  
Pyruvate Orthophosphate Dikinase ◽  
Orthophosphate Dikinase ◽  
Transgenic Lines ◽  
Rna Inhibition ◽  
Target Enzyme ◽  
Flaveria Bidentis

Antisense RNA suppression of either pyruvate, orthophosphate dikinase [EC 2.7.9.1] or NADP malate dehydrogenase [EC 1.1.1.82] gene expression in the C4 dicot Flaveria bidentis L. var. Kuntze produced several independent transgenic lines with leaves showing heritable, mosaic phenotypes. The appearance of these plants was highly variable, with leaves that were either predominantly green, predominantly yellow, or a mixture of the two. The yellow sectors appeared to be clonal in origin. For both sets of transgenic plants, the green and yellow sectors showed a reduction in the activity of the respective target enzyme compared to wild-type leaves. The mRNA of the target enzyme was reduced in both green and yellow sectors of leaves of both types of transformants compared to leaves from wild-type plants. The yellow sectors had decreased amounts of other photosynthetic enzymes on an area basis, but most enzyme activities and electron transport rates were similar to the green sectors on a chlorophyll basis. The mosaic phenotype could not be attributed simply to the degree of suppression of the target enzyme, because we have also obtained uniformly green plants with similar or greater enzyme suppression. The importance of this spatial variability in the effectiveness of the antisense transgenes for the analysis of transgenic plants in general is discussed.

scholarly journals Regulation of plasmid R1 replication: PcnB and RNase E expedite the decay of the antisense RNA, CopA

1997 ◽  
Vol 26 (3) ◽  
pp. 493-504 ◽  
Author(s):  
Fredrik Söderbom ◽  
Uta Binnie ◽  
Millicent Masters ◽  
E. Gerhart H. Wagner
Keyword(s):  
Antisense Rna ◽  
Rnase E ◽  
Plasmid R1
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