A physical and genetic map of the Stigmatella aurantiaca DW4/3.1 chromosome

1993 ◽  
Vol 10 (5) ◽  
pp. 1087-1099 ◽  
Author(s):  
Björn Neumann ◽  
Andreas Pospiech ◽  
Hans Ulrich Schairer
Keyword(s):  
Genetic Map ◽  
Stigmatella Aurantiaca ◽  
Physical And Genetic Map

Physical and genetic map of the Spiroplasma kunkelii CR2-3x chromosome

2006 ◽  
Vol 52 (9) ◽  
pp. 857-867 ◽  
Author(s):  
Ellen L Dally ◽  
Thereza S.L Barros ◽  
Yan Zhao ◽  
ShaoPing Lin ◽  
Bruce A Roe ◽  
...  
Keyword(s):  
Genetic Map ◽  
Gel Electrophoresis ◽  
Southern Hybridization ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Analysis ◽  
Two Dimensional ◽  
Pulsed Field ◽  
Spiroplasma Kunkelii ◽  
Corn Stunt ◽  
Physical And Genetic Map

Spiroplasma kunkelii (class Mollicutes) is the characteristically helical, wall-less bacterium that causes corn stunt disease. A combination of restriction enzyme analysis, pulsed-field gel electrophoresis (PFGE), and Southern hybridization analysis was used to construct a physical and genetic map of the S. kunkelii CR2-3x chromosome. The order of restriction fragments on the map was determined by analyses of reciprocal endonuclease double digests employing I-CeuI, AscI, ApaI, EagI, SmaI, BssHII, BglI, and SalI; adjacent fragments were identified on two-dimensional pulsed-field electrophoresis gels. The size of the chromosome was estimated at 1550 kb. Oligonucleotide pairs were designed to prime the amplification of 26 S. kunkelii gene sequences in the polymerase chain reaction (PCR). Using PCR amplicons as probes, the locations of 27 S. kunkelii putative single-copy genes were positioned on the map by Southern hybridization analyses of chromosomal fragments separated in PFGE. The nucleotide sequence of the single ribosomal RNA operon was determined and its location mapped to a chromosomal segment bearing recognition sites for SalI, SmaI, EagI, and I-CeuI.Key words: Spiroplasma kunkelii CR2-3x, corn stunt spiroplasma, mollicutes, genome mapping, two-dimensional pulsed-field gel electrophoresis.

A detailed physical and genetic map of two R.ColBM IncFIII plasmids

Plasmid ◽  
1984 ◽  
Vol 11 (1) ◽  
pp. 105-108 ◽  
Author(s):  
B. Massie ◽  
Doris Dea ◽  
A. Sasarman
Keyword(s):  
Genetic Map ◽  
Physical And Genetic Map

scholarly journals Physical and genetic map ofEnterococcus faeciumATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci

2002 ◽  
Vol 207 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Kozue Oana ◽  
Yukie Okimura ◽  
Yoshiyuki Kawakami ◽  
Nobuaki Hayashida ◽  
Makoto Shimosaka ◽  
...  
Keyword(s):  
Genetic Map ◽  
Genomic Diversity ◽  
Physical And Genetic Map

scholarly journals A combined physical and genetic map of Pseudomonas aeruginosa PAO

1990 ◽  
Vol 136 (12) ◽  
pp. 2351-2357 ◽  
Author(s):  
E. Ratnaningsih ◽  
S. Dharmsthiti ◽  
V. Krishnapillai ◽  
A. Morgan ◽  
M. Sinclair ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Genetic Map ◽  
Physical And Genetic Map

A physical and genetic map of the

1996 ◽  
Vol 252 (3) ◽  
pp. 255 ◽  
Author(s):  
Brigitte Bathe ◽  
J. Kalinowski ◽  
Alfred Pühler
Keyword(s):  
Genetic Map ◽  
Physical And Genetic Map

scholarly journals A porcine BAC library with tenfold genome coverage: a resource for physical and genetic map integration

2001 ◽  
Vol 12 (6) ◽  
pp. 472-474 ◽  
Author(s):  
Scott C. Fahrenkrug ◽  
Gary A. Rohrer ◽  
Brad A. Freking ◽  
Timothy P.L. Smith ◽  
Kazutoyo Osoegawa ◽  
...  
Keyword(s):  
Genetic Map ◽  
Bac Library ◽  
Genome Coverage ◽  
Physical And Genetic Map ◽  
Map Integration
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