scholarly journals Functional and structural characterization of a novel mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus reveals its dual substrate specificity

2010 ◽  
Vol 79 (1) ◽  
pp. 76-93 ◽  
Author(s):  
Nuno Empadinhas ◽  
Pedro José Barbosa Pereira ◽  
Luciana Albuquerque ◽  
Joana Costa ◽  
Bebiana Sá-Moura ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Structural Characterization ◽  
Dual Substrate Specificity ◽  
Dual Substrate

scholarly journals Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis

2019 ◽  
Vol 75 (4) ◽  
pp. 299-306
Author(s):  
Jolanta Cieślak ◽  
Akimasa Miyanaga ◽  
Makoto Takaishi ◽  
Fumitaka Kudo ◽  
Tadashi Eguchi
Keyword(s):  
Amino Acids ◽  
Substrate Specificity ◽  
Natural Product ◽  
Structural Characterization ◽  
Biochemical Analysis ◽  
Natural Product Biosynthesis ◽  
Broad Substrate Specificity ◽  
Selective Incorporation ◽  
Polyketide Antibiotic

Adenylation enzymes play an important role in the selective incorporation of the cognate carboxylate substrates in natural product biosynthesis. Here, the biochemical and structural characterization of the adenylation enzyme IdnL7, which is involved in the biosynthesis of the macrolactam polyketide antibiotic incednine, is reported. Biochemical analysis showed that IdnL7 selects and activates several small amino acids. The structure of IdnL7 in complex with an L-alanyl-adenylate intermediate mimic, 5′-O-[N-(L-alanyl)sulfamoyl]adenosine, was determined at 2.1 Å resolution. The structure of IdnL7 explains the broad substrate specificity of IdnL7 towards small L-amino acids.

scholarly journals Dual-substrate Specificity Short Chain Retinol Dehydrogenases from the Vertebrate Retina

2002 ◽  
Vol 277 (47) ◽  
pp. 45537-45546 ◽  
Author(s):  
Françoise Haeseleer ◽  
Geeng-Fu Jang ◽  
Yoshikazu Imanishi ◽  
Carola A. G. G. Driessen ◽  
Masazumi Matsumura ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Short Chain ◽  
Vertebrate Retina ◽  
Dual Substrate Specificity ◽  
Dual Substrate
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