molecular tool
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Author(s):  
Kirsten T. Nijholt ◽  
Pablo I. Sánchez‐Aguilera ◽  
Suzanne N. Voorrips ◽  
Rudolf A. Boer ◽  
B. Daan Westenbrink

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1832
Author(s):  
Abdelaziz Ghanemi ◽  
Mayumi Yoshioka ◽  
Jonny St-Amand

The numerous exercise benefits for health as well as applications for diseases has lead to exercise being prescribed in many pathological conditions. Secreted protein acidic and rich in cysteine (SPARC) gene expression is stimulated by exercise and SPARC has been suggested as a molecular mediator of exercise. Therefore, we suggest using this property for personalized medicine. This can be achieved by prescribing the exercise with a pattern (duration, intensity, etc.) that corresponds to the optimum SPARC/Sparc expression. We expect this approach to optimize the exercise therapy in both the preventive and curative contexts. In the research field, measuring exercise -dependent expression of Sparc would represent a molecular tool to further optimize the selection of exercise animal models as well.


PhytoKeys ◽  
2021 ◽  
Vol 183 ◽  
pp. 95-105
Author(s):  
Anton Glushchenko ◽  
Evgeniy Gusev ◽  
Yevhen Maltsev ◽  
John Patrick Kociolek ◽  
Irina Kuznetsova ◽  
...  

A new cymbelloid diatom species from the genus Cymbopleura (Krammer) Krammer is described on the basis of molecular and morphological investigations. Cymbopleura natellia Glushchenko, Kulikovskiy & Kociolek, sp. nov. is, on the basis of results with molecular data, close to C. naviculiformis (Auerswald ex Heiberg) Krammer. The two species differ both by molecular distance and morphological features. Morphologically, C. natelliasp. nov. is compared with several other species in the genus. This work is a pioneer investigation of cymbelloid taxa using molecular tool from Transbaikal area.


2021 ◽  
Author(s):  
Satoshi Watanabe ◽  
Yuta Nihongaki ◽  
Kie Itoh ◽  
Shigeki Watanabe ◽  
Takanari Inoue

Organelles vitally achieve multifaceted functions to maintain cellular homeostasis. Genetic and pharmacological approaches to manipulate individual organelles are powerful in probing their physiological roles. However, many of them are either slow in action, limited to certain organelles, or rely on toxic agents. Here, we designed a generalizable molecular tool utilizing phospholipase A/acyltransferases (PLAATs) for rapid induction of organelle defunctionalization via remodeling of the membrane phospholipid composition. In particular, we identified a minimal, fully catalytic PLAAT with no unfavorable side effects. Chemically-induced translocation of the engineered PLAAT to the mitochondria surface resulted in their rapid deformation in a phospholipase activity dependent manner, followed by loss of luminal proteins as well as dissipated membrane potential, thus invalidating the functionality. To demonstrate wide applicability, we then adapted the molecular tool in peroxisomes, and observed leakage of matrix-resident functional proteins. The technique was compatible with optogenetic control, viral delivery and operation in primary neuronal cultures. Due to such versatility, the PLAAT strategy should present a novel utility in organelle biology of diverse contexts.


Author(s):  
Taewook Seo ◽  
Jihyo Kim ◽  
Ho-Chul Shin ◽  
Jung Gi Kim ◽  
Shinyeong Ju ◽  
...  
Keyword(s):  

Author(s):  
Anelize Ramos ◽  
Leonardo Fernandes ◽  
Franciane Batista ◽  
Maria Risoleta Freire Marques ◽  
Jacó Joaquim Mattos ◽  
...  

G-type immunoglobulins (IgGs) are extensively used in the pharmaceutical industry against various diseases, being also crucial in multiple immunoassays. The production of secondary monoclonal antibodies (Abs) for IgG detection is not cost-effective, while polyclonal antibody production still depends on laboratory animals, which raises concerns regarding animal welfare. As alternatives, bacterial proteins (A and G) have been widely exploited; however, several difficulties are encountered regarding their use for IgG detection and purification. The widespread use of IgGs in the pharmaceutical industry and the increasing number and variety of new Abs entering the market impose the need to develop new detection and purification strategies. The TRIM21 protein is a soluble intracellular IgG receptor that binds to the Fc region of many species with high affinity. We created a chimeric protein containing a mutated form of the C-terminal domain of mouse TRIM21 linked to a streptavidin moiety to detect IgGs from a wide range of species. The protein is promptly produced by heterologous expression and consists of an improved molecular tool, expanding the portfolio of Ab-affinity ligands for immunoassays.


Plant Disease ◽  
2021 ◽  
Author(s):  
Vanessa Tremblay ◽  
Debra L McLaren ◽  
Yong Min Kim ◽  
Stephen Strelkov ◽  
Robert Conner ◽  
...  

The large-scale deployment of Rps (resistance to Phytophthora sojae) genes in soybean has led to the rapid evolution of the virulence profile (pathotype) of P. sojae populations. Determining the pathotypes of P. sojae isolates is important in selecting soybean germplasm carrying the proper Rps, but this process is fastidious and requires specific expertise. In this work, we used a recently developed molecular assay to assess the pathotypes of P. sojae isolates obtained throughout the provinces of Québec, Ontario and Manitoba. In preliminary assays, the molecular tool showed equivalent prediction of the pathotypes as a phenotyping assay and proved to be much faster to apply while eliminating intermediate values. Following the analysis of nearly 300 isolates, 24 different pathotypes were detected in Québec and Ontario, compared to only eight in Manitoba, where soybean culture is more recent. Pathotype 1a, 1c, 1d was predominant in Québec, while 1a, 1b, 1c, 1d, 1k was the most common in Manitoba. Overall, the results showed that 98 and 86% of the isolates carried pathotype 1a or 1c, respectively, suggesting that Rps1a and Rps1c were no longer effective in Canada. Based on the history of soybean varieties used in surveyed fields, it was found that 84% of them contained Rps genes that were no longer resistant against the pathotypes of the isolates found in the fields. While highlighting an easier and more precise option to assess pathotypes, this study presents the first pan-Canadian survey of P. sojae and stresses the importance of carefully managing the declining sources of resistance.


2021 ◽  
Vol 9 (7) ◽  
pp. 1351
Author(s):  
Joaquin I. Rilling ◽  
Fumito Maruyama ◽  
Michael J. Sadowsky ◽  
Jacquelinne J. Acuña ◽  
Milko A. Jorquera

Azospirillum-based plant and soil inoculants are widely used in agriculture. The inoculated Azospirillum strains are commonly tracked by both culture-dependent and culture-independent methods, which are time-consuming or expensive. In this context, clustered regularly interspaced short palindromic repeats (CRISPR) loci structure is unique in the bacterial genome, including some Azospirillum species. Here, we investigated the use of CRISPR loci to track specific Azospirillum strains in soils systems by PCR. Primer sets for Azospirillum sp. strain B510 were designed and evaluated by colony and endpoint PCR. The CRISPRloci-PCR approach was standardized for Azospirillum sp. strain B510, and its specificity was observed by testing against 9 different Azospirillum strains, and 38 strains of diverse bacterial genera isolated from wheat plants. The CRISPRloci-PCR approach was validated in assays with substrate and wheat seedlings. Azospirillum sp. strain B510 was detected after of two weeks of inoculation in both sterile and nonsterile substrates as well as rhizosphere grown in sterile substrate. The CRISPRloci-PCR approach was found to be a useful molecular tool for specific tracking of Azospirillum at the strain level. This technique can be easily adapted to other microbial inoculants carrying CRISPR loci and can be used to complement other microbiological techniques.


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