GD2 Ganglioside-Specific Monoclonal Antibody Reacts with Murine Cytotoxic T Lymphocytes Reactive with FBL-3N Erythroleukaemia

1994 ◽  
Vol 40 (5) ◽  
pp. 557-563 ◽  
Author(s):  
A. YOSHIMURA ◽  
K. TAKAMIYA ◽  
I. KATO ◽  
E. NAKAYAMA ◽  
H. SHIKU ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Specific Monoclonal Antibody ◽  
Gd2 Ganglioside

A new determinant, Qa-m208, detected on T lymphocytes and transfected L cells by a Kb-specific monoclonal antibody

1984 ◽  
Vol 19 (6) ◽  
pp. 461-474 ◽  
Author(s):  
Margriet Oudshoorn-Snoek ◽  
Andrew L. Mellor ◽  
Richard A. Flavell ◽  
Peter Demand
Keyword(s):  
Monoclonal Antibody ◽  
T Lymphocytes ◽  
Specific Monoclonal Antibody ◽  
L Cells

scholarly journals CD8+ Lymphocytes Do Not Mediate Protection against Acute Superinfection 20 Days after Vaccination with a Live Attenuated Simian Immunodeficiency Virus

2005 ◽  
Vol 79 (19) ◽  
pp. 12264-12272 ◽  
Author(s):  
Richard Stebbings ◽  
Neil Berry ◽  
Herman Waldmann ◽  
Pru Bird ◽  
Geoff Hale ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Lymphocytes ◽  
Simian Immunodeficiency Virus ◽  
Cytotoxic T Lymphocytes ◽  
T Lymphocyte ◽  
Lymphoid Tissue ◽  
Human Immunoglobulin ◽  
Wild Type ◽  
Lymphocyte Depletion ◽  
Immunodeficiency Virus

ABSTRACT In order to test the hypothesis that CD8+ cytotoxic T lymphocytes mediate protection against acute superinfection, we depleted >99% of CD8+ lymphocytes in live attenuated simian immunodeficiency virus macC8 (SIVmacC8) vaccinees from the onset of vaccination, maintained that depletion for 20 days, and then challenged with pathogenic, wild-type SIVmacJ5. Vaccinees received 5 mg per kg of humanized anti-CD8 monoclonal antibody (MAb) 1 h before inoculation, followed by the same dose again on days 3, 7, 10, 13, and 17. On day 13, peripheral CD8+ T lymphocytes were >99% depleted in three out of four anti-CD8 MAb-treated vaccinees. At this time attenuated SIVmacC8 viral RNA loads in anti-CD8 MAb-treated vaccinees were significantly higher than control vaccinees treated contemporaneously with nonspecific human immunoglobulin. Lymphoid tissue CD8+ T lymphocyte depletion was >99% in three out of four anti-CD8 MAb-treated vaccinees on the day of wild-type SIVmacJ5 challenge. All four control vaccinees and three out of four anti-CD8 MAb-treated vaccinees were protected against detectable superinfection with wild-type SIVmacJ5. Although superinfection with wild-type SIVmacJ5 was detected at postmortem in a single anti-CD8 MAb-treated vaccinee, this did not correlate with the degree of preceding CD8+ T lymphocyte depletion. Clearance of attenuated SIVmacC8 viremia coincided with recovery of normal CD8+ T lymphocyte counts between days 48 and 76. These results support the view that cytotoxic T lymphocytes are important for host-mediated control of SIV primary viremia but do not indicate a central role in protection against acute superinfection conferred by inoculation with live attenuated SIV.

C1.7 MONOCLONAL ANTIBODY DESIGNATES HIGH-AVIDITY CD4+ CYTOTOXIC T LYMPHOCYTES INVOLVED IN CLINICAL HEART REJECTION

1998 ◽  
Vol 66 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Nancy E.M. van Emmerik ◽  
Christiaan J. Knoop ◽  
Leonard M.B. Vaessen ◽  
Aggie H.M.M. Balk ◽  
Bas Mochtar ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
High Avidity

Bispecific Monoclonal Antibody Targeted Cytotoxic T Lymphocytes can Recycle

T Lymphocytes ◽  
1992 ◽  
pp. 247-249
Author(s):  
J. A. C. Voorthuis ◽  
E. Braakman ◽  
C. P. M. Ronteltap ◽  
N. E. B. A. M. van Esch ◽  
R. L. H. Bolhuis
Keyword(s):  
Monoclonal Antibody ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Bispecific Monoclonal Antibody

scholarly journals TLiSA1, a human T lineage-specific activation antigen involved in the differentiation of cytotoxic T lymphocytes and anomalous killer cells from their precursors.

1985 ◽  
Vol 161 (5) ◽  
pp. 1063-1078 ◽  
Author(s):  
G F Burns ◽  
T Triglia ◽  
J A Werkmeister ◽  
C G Begley ◽  
A W Boyd
Keyword(s):  
Monoclonal Antibody ◽  
T Cells ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Sodium Dodecyl ◽  
Interleukin 2 ◽  
Target Cells ◽  
Scatchard Analysis ◽  
Human T Cells ◽  
Activation Antigen

The characteristics of a novel T lineage-specific activation antigen, termed TLiSA1, are described. The antigen was detected with a mouse monoclonal antibody, LeoA1, that was raised against activated human T cells generated in mixed lymphocyte culture (MLC). The antigen became strongly expressed on T cells 48-72 h after stimulation with phytohemagglutinin, and retained expression on MLC-activated T cells after 10 d of culture. The antigen was absent from a range of human T, B, myeloid, fibroblast, and tumour cell lines, but was present on the surface of the interleukin 2 (IL-2)-dependent gibbon cell line MLA-144. Analysis of the antigen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of immunoprecipitates obtained from activated human T cells demonstrated a broad band in the region of 70 kD, whereas precipitates obtained from MLA-144 revealed a single narrow band of 95 kD. The molecule was expressed with a maximum density of 66,000 copies per cell on the surface of MLC-activated T cell blasts, as assessed by Scatchard analysis. TLiSA1 was distinguished from the IL-2 receptor bound by the anti-Tac monoclonal antibody by demonstrating that the antigens did not comodulate or coprecipitate, and by constructing an IL-2-independent human T X T hybrid that expressed the TLiSA1 but not the Tac antigen. MLC with B lymphoblasts was used to generate cytotoxic T lymphocytes (CTL) specific for the stimulating cell, and anomalous killer (AK) cells able to kill melanoma target cells. The presence of LeoA1 or F(ab')2 fragments of the antibody from the beginning of coculture did not affect proliferation in these cultures, but did inhibit the induction of both CTL and AK cells from their precursors. This inhibition of differentiation by LeoA1 was confirmed under conditions of limiting dilution, where it was shown that the antibody reduced the frequency of CTL produced, and greatly (fourfold) reduced the frequency of AK cells generated from their precursors. We discuss the possibility that human CTL may express a differentiation factor receptor that is distinct from the receptor for IL-2.

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