Identification and characterization of a novel synthetic peptide substrate specific for Src-family protein tyrosine kinases

2009 ◽  
Vol 45 (6) ◽  
pp. 587-592 ◽  
Author(s):  
KIT S. LAM ◽  
JINZI WU ◽  
QIANG LOU
Keyword(s):  
Tyrosine Kinases ◽  
Synthetic Peptide ◽  
Protein Tyrosine Kinases ◽  
Peptide Substrate ◽  
Protein Tyrosine ◽  
Family Protein ◽  
Identification And Characterization

Association between B-lymphocyte membrane immunoglobulin and multiple members of the Src family of protein tyrosine kinases

1992 ◽  
Vol 12 (5) ◽  
pp. 2315-2321
Author(s):  
M A Campbell ◽  
B M Sefton
Keyword(s):  
B Cells ◽  
B Cell ◽  
Tyrosine Kinases ◽  
B Lymphocyte ◽  
Protein Tyrosine Kinases ◽  
Cell Type ◽  
Protein Tyrosine ◽  
Membrane Immunoglobulin ◽  
Family Protein

Treatment of B lymphocytes with antibodies to membrane immunoglobulin (Ig) stimulates protein tyrosine phosphorylation. We have examined the phosphorylation in vitro of proteins associated with membrane Ig. The Src family protein tyrosine kinases p53/56lyn, p59fyn, and p56lck are associated with membrane Ig in spleen B cells and B-cell lines and undergo phosphorylation in vitro. The pattern of expression of Src family protein tyrosine kinases in B cells varied. Our studies suggest that multiple kinases can potentially interact with membrane Ig and that within any one B-cell type, all of the Src family kinases expressed can be found in association with membrane Ig. We also observed that the Ig-associated Ig alpha protein, multiple forms of Ig beta, and proteins of 100 and 25 kDa were tyrosine phosphorylated in vitro. The 100- and 25-kDa proteins remain unidentified.

Isolation and characterization of a human gene that encodes a new subclass of protein tyrosine kinases

Gene ◽  
1992 ◽  
Vol 110 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Andreas Bräuminger ◽  
Uwe Holtrich ◽  
Klaus Strebhardt ◽  
Helga Rübsamen-Waigmann
Keyword(s):  
Tyrosine Kinases ◽  
Human Gene ◽  
Protein Tyrosine Kinases ◽  
Protein Tyrosine ◽  
Isolation And Characterization

scholarly journals Allosteric networks governing regulation and catalysis of Src-family protein tyrosine kinases: Implications for disease-associated kinases

2010 ◽  
Vol 37 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Heung-Chin Cheng ◽  
Timothy M Johnson ◽  
Ryan D Mills ◽  
Yuh-Ping Chong ◽  
Khai-Chew Chan ◽  
...  
Keyword(s):  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Protein Tyrosine ◽  
Family Protein

scholarly journals Association between B-lymphocyte membrane immunoglobulin and multiple members of the Src family of protein tyrosine kinases.

1992 ◽  
Vol 12 (5) ◽  
pp. 2315-2321 ◽  
Author(s):  
M A Campbell ◽  
B M Sefton
Keyword(s):  
B Cells ◽  
B Cell ◽  
Tyrosine Kinases ◽  
B Lymphocyte ◽  
Protein Tyrosine Kinases ◽  
Cell Type ◽  
Protein Tyrosine ◽  
Membrane Immunoglobulin ◽  
Family Protein

Treatment of B lymphocytes with antibodies to membrane immunoglobulin (Ig) stimulates protein tyrosine phosphorylation. We have examined the phosphorylation in vitro of proteins associated with membrane Ig. The Src family protein tyrosine kinases p53/56lyn, p59fyn, and p56lck are associated with membrane Ig in spleen B cells and B-cell lines and undergo phosphorylation in vitro. The pattern of expression of Src family protein tyrosine kinases in B cells varied. Our studies suggest that multiple kinases can potentially interact with membrane Ig and that within any one B-cell type, all of the Src family kinases expressed can be found in association with membrane Ig. We also observed that the Ig-associated Ig alpha protein, multiple forms of Ig beta, and proteins of 100 and 25 kDa were tyrosine phosphorylated in vitro. The 100- and 25-kDa proteins remain unidentified.

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