Adventitious root formation 'in vitro' in apple rootstocks (Malus pumila) I. Factors affecting the length of the auxin-sensitive phase in M.9

Stem expiants of the rhododendron cultivar Catawbiense Album rooted more easily in vitro than those of Pink Pearl, agreeing with the experience of nursery practice. Rooting occurred only on segments of young soft shoots and was strongly promoted when the expiants were placed inverted on the medium. Continuous light inhibited, whereas continuous darkness promoted rooting. Rooting occurred only in the presence of an auxin together with a sugar in the culture medium. There was no evidence that mineral nutrition and temperatures between 21 degrees and 29 degrees C. play an important role.-Univ. Wageningen. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Download Full-text

Advance of adventitious root formation and production, and saponin accumulation by in vitro cultures of Giao Co Lam (Gynostemma pentaphyllum)

Acta Horticulturae ◽

10.17660/actahortic.2018.1224.15 ◽

2018 ◽

pp. 109-118

Author(s):

P.C. Khai ◽

T.V. Minh

Keyword(s):

Adventitious Root ◽

Root Formation ◽

Adventitious Root Formation ◽

In Vitro Cultures ◽

Gynostemma Pentaphyllum

Download Full-text

Melatonin activates adventitious root formation by promoting the function of MdWOX11 in apple

10.21203/rs.3.rs-29239/v1 ◽

2020 ◽

Author(s):

Jiangping Mao ◽

Chundong Niu ◽

Ke Li ◽

Muhammad Mobeen Tahir ◽

Mingyu Han ◽

...

Keyword(s):

Adventitious Root ◽

Root Formation ◽

Zeatin Riboside ◽

Adventitious Root Formation ◽

Control Group ◽

Endogenous Hormones ◽

Apple Rootstocks ◽

Phenotypic Analysis ◽

Set Up ◽

Induction Stage

Abstract Background Melatonin (MT) plays a key role in the plant growth and development, however, whether MT involved in apple adventitious root (AR) development is not entirely understood. In present study, we set up four MT-treated groups at different times according to the stages of AR development and one control group in Malus domestica (MP), endogenous hormones levels of MT, auxin (IAA), zeatin-riboside (ZR), gibberellins (GA1 + 3), abscisic acid (ABA) were analyzed in five groups, then the expression of MT signal, IAA synthesis, transport, and signal transduction, cell cycle, and root development related genes were measured by RT-qPCR, the function of MdWOX11 was analyzes by technology of apple transgenic. Results Advance of AR development by MT is depended on its promotion of stage of AR induction at 0–2 d in the apple rootstocks. MT-treated increased IAA levels, there were crosstalk between MT and IAA during inducing AR formation. Expression analysis exposed that the expression of MdWOX11 induced by MT-treatment, and positively regulated AR formation in apple. Furthermore, MdWOX11 over-expressed lines produced more ARs than ‘GL3’, phenotypic analysis indicated that MdWOX11 over-expressed lines were more sensitive to exogenous MT-treatment than ‘GL3’, it was also indicated that MdWOX11 regulated AR formation in response to MT in apple rootstock. Conclusions MT promotes AR formation mainly during AR induction stage by inducing IAA levels as well as by up-regulation of MdWOX11.

Download Full-text