Melatonin activates adventitious root formation by promoting the function of MdWOX11 in apple

Abstract Background Melatonin (MT) plays a key role in the plant growth and development, however, whether MT involved in apple adventitious root (AR) development is not entirely understood. In present study, we set up four MT-treated groups at different times according to the stages of AR development and one control group in Malus domestica (MP), endogenous hormones levels of MT, auxin (IAA), zeatin-riboside (ZR), gibberellins (GA1 + 3), abscisic acid (ABA) were analyzed in five groups, then the expression of MT signal, IAA synthesis, transport, and signal transduction, cell cycle, and root development related genes were measured by RT-qPCR, the function of MdWOX11 was analyzes by technology of apple transgenic. Results Advance of AR development by MT is depended on its promotion of stage of AR induction at 0–2 d in the apple rootstocks. MT-treated increased IAA levels, there were crosstalk between MT and IAA during inducing AR formation. Expression analysis exposed that the expression of MdWOX11 induced by MT-treatment, and positively regulated AR formation in apple. Furthermore, MdWOX11 over-expressed lines produced more ARs than ‘GL3’, phenotypic analysis indicated that MdWOX11 over-expressed lines were more sensitive to exogenous MT-treatment than ‘GL3’, it was also indicated that MdWOX11 regulated AR formation in response to MT in apple rootstock. Conclusions MT promotes AR formation mainly during AR induction stage by inducing IAA levels as well as by up-regulation of MdWOX11.

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Melatonin promotes adventitious root formation in apple by promoting the function of MdWOX11

BMC Plant Biology ◽

10.1186/s12870-020-02747-z ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Jiangping Mao ◽

Chundong Niu ◽

Ke Li ◽

Shiyue Chen ◽

Muhammad Mobeen Tahir ◽

...

Keyword(s):

Adventitious Root ◽

Zeatin Riboside ◽

Adventitious Root Formation ◽

Control Group ◽

Apple Rootstocks ◽

Phenotypic Analysis ◽

Malus Prunifolia ◽

Treatment Groups ◽

Transgenic Lines ◽

Transgenic Apple

Abstract Background Melatonin (MT) is important for plant growth and development; however, it is not known whether MT is involved in apple adventitious root (AR) development. In this study, we treated Malus prunifolia (MP) at four different stages of AR development, and analyzed the level of the endogenous hormones MT, auxin (IAA), zeatin-riboside (ZR), abscisic acid (ABA), and gibberellins (GA1 + 3) in all four treatment groups and the untreated control group. The expression of MT, IAA biosynthesis, transport and signal transduction, the cell cycle, and root development related genes were quantified by RT-qPCR. The function of MdWOX11 was analyzed in transgenic apple plants. Results The promotion of AR development by MT was dependent on the stage of AR induction between 0 and 2 d in apple rootstocks. MT-treatment increased the level of IAA and crosstalk existed between MT and IAA during AR formation. The expression of MdWOX11 was induced by MT treatment and positively regulated AR formation in apple. Furthermore, transgenic lines that overexpressed MdWOX11 lines produced more ARs than ‘GL3’. Phenotypic analysis indicated that MdWOX11 overexpression lines were more sensitive to exogenous MT treatment than ‘GL3’, suggesting that MdWOX11 regulates AR formation in response to MT in apple rootstock. Conclusions MT promotes AR formation mainly during the AR induction stage by inducing IAA levels and upregulating MdWOX11.

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Melatonin promotes adventitious root formation in apple by promoting the function of MdWOX11

10.21203/rs.3.rs-29239/v2 ◽

2020 ◽

Author(s):

Jiangping Mao ◽

Chundong Niu ◽

Ke Li ◽

Shiyue Chen ◽

Muhammad Mobeen Tahir ◽

...

Keyword(s):

Adventitious Root ◽

Zeatin Riboside ◽

Adventitious Root Formation ◽

Control Group ◽

Apple Rootstocks ◽

Phenotypic Analysis ◽

Malus Prunifolia ◽

Treatment Groups ◽

Expression Of Genes ◽

Transgenic Lines

Abstract Background: Melatonin (MT) is important for plant growth and development; however, it is not known whether MT is involved in apple adventitious root (AR) development. In this study, we treated Malus prunifolia (MP) at four different stages of AR development, and analysed the level of the endogenous hormones MT, auxin (IAA), zeatin-riboside (ZR), gibberellins (GA1+3) and abscisic acid (ABA) in all four treatment groups and in the untreated control group. The expression of genes related to MT, IAA biosynthesis, transport and signal transduction, the cell cycle, and root development was quantified by RT-qPCR, and the function of MdWOX11 was analysed in transgenic apple plants.Results: The promotion of AR development by MT was dependent on the stage of AR induction between 0–2 d in apple rootstocks. MT-treatment increased the level of IAA and crosstalk existed between MT and IAA during AR formation. The expression of MdWOX11 was induced by MT treatment and positively regulated AR formation in apple. Furthermore, transgenic lines that overexpressed MdWOX11 lines produced more ARs than ‘GL3’. Phenotypic analysis indicated that MdWOX11 overexpression lines were more sensitive to exogenous MT treatment than ‘GL3’, suggesting that MdWOX11 regulates AR formation in response to MT in apple rootstock.Conclusions: MT promotes AR formation mainly during the AR induction stage by inducing IAA levels and upregulating MdWOX11.

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Melatonin promotes adventitious root formation in apple by promoting the function of MdWOX11

10.21203/rs.3.rs-29239/v3 ◽

2020 ◽

Author(s):

Jiangping Mao ◽

Chundong Niu ◽

Ke Li ◽

Shiyue Chen ◽

Muhammad Mobeen Tahir ◽

...

Keyword(s):

Adventitious Root ◽

Zeatin Riboside ◽

Adventitious Root Formation ◽

Control Group ◽

Apple Rootstocks ◽

Phenotypic Analysis ◽

Malus Prunifolia ◽

Treatment Groups ◽

Transgenic Lines ◽

Transgenic Apple

Abstract Background: Melatonin (MT) is important for plant growth and development; however, it is not known whether MT is involved in apple adventitious root (AR) development. In this study, we treated Malus prunifolia (MP) at four different stages of AR development, and analyzed the level of the endogenous hormones MT, auxin (IAA), zeatin-riboside (ZR), abscisic acid (ABA), and gibberellins (GA1+3) in all four treatment groups and the untreated control group. The expression of MT, IAA biosynthesis, transport and signal transduction, the cell cycle, and root development related genes were quantified by RT-qPCR. The function of MdWOX11 was analyzed in transgenic apple plants.Results: The promotion of AR development by MT was dependent on the stage of AR induction between 0–2 d in apple rootstocks. MT-treatment increased the level of IAA and crosstalk existed between MT and IAA during AR formation. The expression of MdWOX11 was induced by MT treatment and positively regulated AR formation in apple. Furthermore, transgenic lines that overexpressed MdWOX11 lines produced more ARs than ‘GL3’. Phenotypic analysis indicated that MdWOX11 overexpression lines were more sensitive to exogenous MT treatment than ‘GL3’, suggesting that MdWOX11 regulates AR formation in response to MT in apple rootstock.Conclusions: MT promotes AR formation mainly during the AR induction stage by inducing IAA levels and upregulating MdWOX11.

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Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽

10.21273/hortsci15212-20 ◽

2020 ◽

Vol 55 (9) ◽

pp. 1463-1467 ◽

Cited By ~ 3

Author(s):

Benjamin E. Deloso ◽

Anders J. Lindström ◽

Frank A. Camacho ◽

Thomas E. Marler

Keyword(s):

Butyric Acid ◽

Root Development ◽

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

The Other ◽

Control Group ◽

Stem Cuttings ◽

Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

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