Flow-Cytometric Analysis of Erythrocytic Blood Group A Antigen Density Profile

Abstract Objectives RBC alloantibodies can lead to ABO grouping discrepancies unrelated to A or B antigens or antibodies posing challenges in the blood bank testing. Routine blood bank testing and flow cytometry were used to immunophenotype reagent cells and elucidate the cause of ABO discrepancies in two patients. Methods ABO discrepancy was identified in two patients after transfusion with several units of RBCs. For both patients, the pretransfusion type and screen demonstrated blood group A. Eight and 16 days later, both patients showed an apparent antibody to reagent group A cells, which prompted additional study with patients’ samples and flow cytometric testing of commercial reagent cells. Results In both patients’ specimens, posttransfusion evaluation demonstrated an emerging antibody to the Kell antigen (K). The RBCs of both patients typed negative for K, and both were transfused with K-positive RBCs. Flow cytometric analysis of reagent RBCs demonstrated that five of seven lot numbers were positive for K. Conclusions Emerging anti-K antibody led to agglutination of the K-positive reagent A1 cells, highlighting the importance of considering RBC alloantibodies and the composition of reagent cells when interpreting cases with an apparent ABO grouping discrepancy.

Download Full-text

Red blood cell blood group A antigen level affects the ability of heparin and PfEMP1 antibodies to disrupt Plasmodium falciparum rosettes

Malaria Journal ◽

10.1186/s12936-021-03975-w ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Pontus Hedberg ◽

Madle Sirel ◽

Kirsten Moll ◽

Mpungu Steven Kiwuwa ◽

Petter Höglund ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Blood Group ◽

Experimental Studies ◽

Surface Antigens ◽

Adverse Outcomes ◽

Single Group ◽

Flow Cytometric Assay ◽

Blood Group A ◽

Flow Cytometric ◽

Group A

Abstract Background The histo-blood group ABO system has been associated with adverse outcomes in COVID-19, thromboembolic diseases and Plasmodium falciparum malaria. An integral part of the severe malaria pathogenesis is rosetting, the adherence of parasite infected red blood cells (RBCs) to uninfected RBCs. Rosetting is influenced by the host’s ABO blood group (Bg) and rosettes formed in BgA have previously been shown to be more resilient to disruption by heparin and shield the parasite derived surface antigens from antibodies. However, data on rosetting in weak BgA subgroups is scarce and based on investigations of relatively few donors. Methods An improved high-throughput flow cytometric assay was employed to investigate rosetting characteristics in an extensive panel of RBC donor samples of all four major ABO Bgs, as well as low BgA expressing samples. Results All non-O Bgs shield the parasite surface antigens from strain-specific antibodies towards P. falciparum erythrocyte membrane protein 1 (PfEMP1). A positive correlation between A-antigen levels on RBCs and rosette tightness was observed, protecting the rosettes from heparin- and antibody-mediated disruption. Conclusions These results provide new insights into how the ABO Bg system affects the disease outcome and cautions against interpreting the results from the heterogeneous BgA phenotype as a single group in epidemiological and experimental studies. Graphical Abstract

Download Full-text

609: Reduced Expression of the Blood Group a Antigen is Caused by Allelic Loss and/or Hypermethylation of the ABO Gene on 9Q34.1 in Transitional Cell Carcinoma of the Bladder

The Journal of Urology ◽

10.1016/s0022-5347(18)34849-3 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 166-166

Author(s):

Yoshitomo Chihara ◽

Kiyohide Fujimoto ◽

Yoshihiko Hirao ◽

Kokichi Sugano ◽

Yae Kanai ◽

...

Keyword(s):

Transitional Cell Carcinoma ◽

Cell Carcinoma ◽

Blood Group ◽

Transitional Cell ◽

Allelic Loss ◽

Blood Group A ◽

Group A ◽

Carcinoma Of The Bladder

Download Full-text

Faculty Opinions recommendation of Serum antibodies to blood group A predict survival on PROSTVAC-VF.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717987121.793472395 ◽

2013 ◽

Author(s):

Kevin Yarema

Keyword(s):

Blood Group ◽

Serum Antibodies ◽

Blood Group A ◽

Group A

Download Full-text

Isolation and characterization of novel glycolipids with blood group A-related structures: galactosyl-A and sialosylgalactosyl-A.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47927-6 ◽

1987 ◽

Vol 262 (29) ◽

pp. 14228-14234

Author(s):

H Clausen ◽

S B Levery ◽

E D Nudelman ◽

M Stroud ◽

M E Salyan ◽

...

Keyword(s):

Blood Group ◽

Blood Group A ◽

Isolation And Characterization ◽

Group A

Download Full-text

Immunochemical studies on blood groups. Purification and characterization of radioactive 3H-reduced di- to hexasaccharides produced by alkaline beta-elimination-borohydride 3H reduction of Smith degraded blood group A active glycoproteins.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)39854-x ◽

1984 ◽

Vol 259 (11) ◽

pp. 7178-7186 ◽

Cited By ~ 6

Author(s):

A M Wu ◽

E A Kabat ◽

B Nilsson ◽

D A Zopf ◽

F G Gruezo ◽

...

Keyword(s):

Blood Group ◽

Blood Groups ◽

Purification And Characterization ◽

Blood Group A ◽

Group A ◽

Beta Elimination

Download Full-text

ABO groups can play a role in susceptibility and severity of COVID-19

Egyptian Journal of Bronchology ◽

10.1186/s43168-020-00051-w ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

S. Samra ◽

M. Habeb ◽

R. Nafae

Keyword(s):

Blood Group ◽

Abo Blood Group ◽

Infection Group ◽

Mechanically Ventilated ◽

Blood Group A ◽

Group A ◽

Study Population ◽

Group B ◽

The Relationship ◽

Seriously Ill

Abstract Background A few people infected by the coronavirus become seriously ill, while others show little to no signs of the symptoms, or are asymptomatic. Recent researches are pointing to the fact that the ABO blood group might play an important role in a person’s susceptibility and severity of COVID-19 infection. Aim of the study: try to understand the relationship between ABO groups and COVID-19 (susceptibility and severity). Results A total of (507) patients were included in this study. The study population was divided based on the ABO blood group into types A+, A−, B+, AB, O+, and O−. Blood group A was associated with high susceptibility of infection: group A, 381 (75.1%); and less common in group O, 97 (19.2%), group B, 18 (3.5%), and group AB, 11 (2.2%). The severity of COVID-19 infection was common in non-blood group O where (20 (7.1%), 4 (26.7%), 2 (11%), and 1 (9%) in type A+, A−, B+, and AB, respectively), while in type O 3.1%. And mechanically ventilated patients were 22 (5.9%), 2 (13.4%), 2 (11.1%), and 1 (1%). Mortality was high in blood groups A and B, 16 (4.37%) and 1 (5.5%), respectively, while in blood group O, it was 1%. Conclusion The incidence, severity, and mortality of COVID-19 were common in non-blood group O. While blood group O was protected against COVID-19.

Download Full-text