Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue

2005 ◽  
Vol 73 (2-3) ◽  
pp. 69-78 ◽  
Author(s):  
Takamichi Miyazaki ◽  
Yasuo Kitagawa ◽  
Kazuhiro Toriyama ◽  
Masato Kobori ◽  
Shuhei Torii
1983 ◽  
Vol 49 (3) ◽  
pp. 411-417 ◽  
Author(s):  
Katherine A. Smalley ◽  
Quinton R. Rogers ◽  
James G. Morris

1. The effects of feeding either high-protein (HP) or low-protein (LP) diets between 1.8 and 15 kg live weight (LW) and a low-energy (LE) or high-energy (HE) intake subsequently on the cellularity of muscle and adipose tissue in pigs growing to 75 kg LW were investigated.2. The effects of the nutritional treatments on muscle tissue were assessed from the weight and DNA content of the m. adductor. For adipose tissue the total DNA content and fat cell size of the subcutaneous adipose tissue contained in the left shoulder joint were determined.3. Feeding the LP diets in early life reduced the weight and DNA content of the m. adductor (P < 0.01) and increased fat cell size (P < 0.01) at 15 kg LW.4. Subsequent to 15 kg there was an almost linear increase in muscle DNA with increasing LW, and the difference between pigs from the initial protein treatments progressively diminished and was no longer apparent at 60 kg LW.5. At 30 kg LW, pigs given the LP diets before 15 kg LW contained less DNA in the subcutaneous adipose tissue from the shoulder joint (P < 0.01) and had larger fat cells (P < 0.05) than pigs given the HP diets initially. However, adipose DNA and fat cell size increased with increasing LW and the differences resulting from the initial protein treatments progressively diminished. On the LE and HE treatments subsequent to 15 kg these differences were no longer evident at 45 and 60 kg respectively.6. Pigs given the HE intake subsequent to 15 kg, contained less DNA in muscle tissue (P < 0·05) at 60 and 75 kg LW and had larger fat cells (P < 0·05) at 45, 60 and 75 kg LW, than pigs on the LE treatment.


2009 ◽  
Vol 23 (10) ◽  
pp. 3494-3505 ◽  
Author(s):  
Silvana Baglioni ◽  
Michela Francalanci ◽  
Roberta Squecco ◽  
Adriana Lombardi ◽  
Giulia Cantini ◽  
...  

1987 ◽  
Vol 19 (07) ◽  
pp. 341-342 ◽  
Author(s):  
N. Kuribayashi ◽  
H. Makino ◽  
S. Fujimoto ◽  
A. Kanatsuka ◽  
S. Yoshida

Author(s):  
D. Alzamora ◽  
V. Pardo ◽  
E. Monkus ◽  
M. Novak

In vitro studies on the metabolic characteristics of white subcutaneous adipose tissue obtained from human newborns have shown metabolic differences with those in adults. There is an increase in oxygen consumption, CO2 production from labeled glucose, and increased incorporation of palmitic acid into triglycerides. Glycogen content was high in 2 to 4 hours old neonates.In order to compare the structure of neonate and adult fat, needle biopsies of gluteal subcutaneous adipose tissue were performed in 15 newborns ranging in age from 2 hours to 5 days, and in 4 adults. Tissue fragments and fat cells isolated by collagenase digestion were fixed in 4% osmium tetroxide and 3.5% glutaraldehyde followed by osmium tetroxide in phosphate buffer and embedded in Epon 812.


1983 ◽  
Vol 50 (3) ◽  
pp. 619-626 ◽  
Author(s):  
R. G. Campbell ◽  
A. C. Dunkin

1. The effects of feeding either high-protein (HP) or low-protein (LP) diets between 1·8 and 15 kg live weight (LW) and a low-energy (LE) or high-energy (HE) intake subsequently on the cellularity of muscle and adipose tissue in pigs growing to 75 kg LW were investigated.2. The effects of the nutritional treatments on muscle tissue were assessed from the weight and DNA content of the m. adductor. For adipose tissue the total DNA content and fat cell size of the subcutaneous adipose tissue contained in the left shoulder joint were determined.3. Feeding the LP diets in early life reduced the weight and DNA content of the m. adductor (P < 0·01) and increased fat cell size (P < 0·01) at 15 kg LW.4. Subsequent to 15 kg there was an almost linear increase in muscle DNA with increasing LW, and the difference between pigs from the initial protein treatments progressively diminished and was no longer apparent at 60 kg LW.5. At 30 kg LW, pigs given the LP diets before 15 kg LW contained less DNA in the subcutaneous adipose tissue from the shoulder joint (P < 0·01) and had larger fat cells (P < 0·05) than pigs given the HP diets initially. However, adipose DNA and fat cell size increased with increasing LW and the differences resulting from the initial protein treatments progressively diminished. On the LE and HE treatments subsequent to 15 kg these differences were no longer evident at 45 and 60 kg respectively.6. Pigs given the HE intake subsequent to 15 kg, contained less DNA in muscle tissue (P < 0·05) at 60 and 75 kg LW and had larger fat cells (P < 0·05) at 45, 60 and 75 kg LW, than pigs on the LE treatment.


2019 ◽  
Author(s):  
Frederique Van de Velde ◽  
Margriet Ouwens ◽  
Arsene-Helene Batens ◽  
Samyah Shadid ◽  
Bruno Lapauw ◽  
...  

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 701-P
Author(s):  
PALLAVI VARSHNEY ◽  
BENJAMIN J. RYAN ◽  
CHIWOON AHN ◽  
MICHAEL W. SCHLEH ◽  
JEFFREY F. HOROWITZ

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