Subunit Interactions in Enzyme Catalysis. Kinetic Analysis of Subunit Interactions in the Enzyme l-Phenylalanine Ammonia-Lyase

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.

Download Full-text

Kinetic Analysis of the Oxidative Conversion of methane in Slow Combustion. II. Parametric Characteristics of Directional Conversion

Физика горения и взрыва ◽

10.15372/fgv20200102 ◽

2020 ◽

Keyword(s):

Kinetic Analysis ◽

Oxidative Conversion ◽

Slow Combustion ◽

Conversion Of Methane ◽

Oxidative Conversion Of Methane

Download Full-text

Primary and Secondary Crystallization Kinetic Analysis of Poly(Hexamethylene Succinate)

Journal of Advanced Thermal Science Research ◽

10.15377/2409-5826.2015.02.02.4 ◽

2016 ◽

Vol 2 (2) ◽

pp. 71-76

Author(s):

Zhiyong Wei ◽

Keyword(s):

Kinetic Analysis ◽

Crystallization Kinetic ◽

Secondary Crystallization

Download Full-text

Recent data on the causative agent of pale green dwarf (Acholeplasma laidlawii var. granulum incertae sedis) in Ukraine: pathogenicityand virulence factors and host reactions

Agricultural science and practice ◽

10.15407/agrisp2.01.030 ◽

2015 ◽

Vol 2 (1) ◽

pp. 30-34

Author(s):

K. Korobkova ◽

V. Patyka

Keyword(s):

Phenylalanine Ammonia Lyase ◽

Proteolytic Enzymes ◽

Physiological State ◽

Plant Cells ◽

Temporary Increase ◽

Adhesive Properties ◽

The Impact ◽

Signaling Interactions ◽

Pale Green

Contemporary state of the distribution of mycoplasma diseases of cultivated crops in Ukraine was analyzed. The changes of the physiological state of plant cells under the impact of mollicutes were investigated. It was demonstrated that there is temporary increase in the activity of peroxidase, catalase, polyphenoloxidase, phenylalanine-ammonia-lyase at the early stages of interaction. The adhesive properties are changed in the mollicutes under the impact of plant lectin; there is synthesis of new polypeptides. It was determined that the phytopathogenic acholeplasma is capable of producing a complex of proteolytic enzymes into the culture me- dium. It was concluded that when plant cells are infected with acholeplasma, a number of signaling interactions and metabolic transformations condition the recognition of pathogenesis and ensure the aggregate response of a plant to stress in the form of defense reactions. It was assumed that some specifi cities of the biology of phy- topathogenic acholeplasma determine their avoiding the immune mechanisms of plants and promote long-term persistence of mollicutes.

Download Full-text