Isolation and Characterization of Hydrophobic Proteins (H Proteins) in the Membrane Fraction of Bacillus subtilis. Involvement in Membrane Biosynthesis and the Formation of Biochemically Active Membrane Vesicles by Combining H Proteins with Lipid

1976 ◽  
Vol 71 (2) ◽  
pp. 451-458 ◽  
Author(s):  
Iwao KUSAKA ◽  
Kou HAYAKAWA ◽  
Keiko KANAI ◽  
Sakuzo FUKUI
Keyword(s):  
Bacillus Subtilis ◽  
Membrane Fraction ◽  
Membrane Vesicles ◽  
Isolation And Characterization ◽  
Active Membrane ◽  
Membrane Biosynthesis

Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin

Extremophiles ◽  
2002 ◽  
Vol 6 (6) ◽  
pp. 499-506 ◽  
Author(s):  
Niran Roongsawang ◽  
Jiraporn Thaniyavarn ◽  
Suthep Thaniyavarn ◽  
Takayuki Kameyama ◽  
Mitsuru Haruki ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Isolation And Characterization ◽  
Bacillomycin L

scholarly journals Biochemical Characterization of the C4-Dicarboxylate Transporter DctA from Bacillus subtilis

2010 ◽  
Vol 192 (11) ◽  
pp. 2900-2907 ◽  
Author(s):  
Maarten Groeneveld ◽  
Ruud G. J. Detert Oude Weme ◽  
Ria H. Duurkens ◽  
Dirk Jan Slotboom
Keyword(s):  
Bacillus Subtilis ◽  
Biochemical Characterization ◽  
Krebs Cycle ◽  
Membrane Vesicles ◽  
Positive Bacterium ◽  
Proton Symport ◽  
Negative Membrane Potential ◽  
Isolated Membrane Vesicles

ABSTRACT Bacterial secondary transporters of the DctA family mediate ion-coupled uptake of C4-dicarboxylates. Here, we have expressed the DctA homologue from Bacillus subtilis in the Gram-positive bacterium Lactococcus lactis. Transport of dicarboxylates in vitro in isolated membrane vesicles was assayed. We determined the substrate specificity, the type of cotransported ions, the electrogenic nature of transport, and the pH and temperature dependence patterns. DctA was found to catalyze proton-coupled symport of the four C4-dicarboxylates from the Krebs cycle (succinate, fumurate, malate, and oxaloacetate) but not of other mono- and dicarboxylates. Because (i) succinate-proton symport was electrogenic (stimulated by an internal negative membrane potential) and (ii) the divalent anionic form of succinate was recognized by DctA, at least three protons must be cotransported with succinate. The results were interpreted in the light of the crystal structure of the homologous aspartate transporter GltPh from Pyrococcus horikoshii.

Production, isolation and characterization of [Leu4]- and [Ile4]surfactins from Bacillus subtilis

1995 ◽  
Vol 2 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Jean-Marc Bonmatin ◽  
Henri Labb� ◽  
Isabelle Grangemard ◽  
Fran�oise Peypoux ◽  
R�gine Maget-Dana ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Inhibitors of the Essential Histidine Kinase, YycG in Bacillus subtilis and Staphylococcus aureus

2003 ◽  
Vol 56 (12) ◽  
pp. 1045-1052 ◽  
Author(s):  
TAKAFUMI WATANABE ◽  
YOSHIKI HASHIMOTO ◽  
KANEYOSHI YAMAMOTO ◽  
KIYO HIRAO ◽  
AKIRA ISHIHAMA ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Histidine Kinase ◽  
Isolation And Characterization ◽  
And Staphylococcus Aureus
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