scholarly journals Kinetics of Coupled Reactions Catalyzed by Aspartate Aminotransferase and Glutamate Dehydrogenase

1982 ◽  
Vol 121 (3) ◽  
pp. 511-517 ◽  
Author(s):  
Costantino SALERNO ◽  
Judit OVADI ◽  
Tamas KELETI ◽  
Paolo FASELLA
Keyword(s):  
Glutamate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Coupled Reactions ◽  
Kinetics Of

Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer.

1976 ◽  
Vol 22 (10) ◽  
pp. 1614-1617 ◽  
Author(s):  
J P Bretaudiere ◽  
H T Phung ◽  
M Bailly
Keyword(s):  
Glutamate Dehydrogenase ◽  
Continuous Flow ◽  
Kinetic Scheme ◽  
Fixed Time ◽  
Sample Volume ◽  
Enzymatic Determination ◽  
Coupled Reactions ◽  
Continuous Flow Method ◽  
Enzymatic Micromethod

Abstract A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed-time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.

Effect of cryosolvent on transient kinetics of the glutamate dehydrogenase reaction

Biochemistry ◽  
1982 ◽  
Vol 21 (26) ◽  
pp. 6695-6699
Author(s):  
Alan H. Colen ◽  
Robert E. Johnson ◽  
Harvey F. Fisher
Keyword(s):  
Glutamate Dehydrogenase ◽  
Transient Kinetics ◽  
Dehydrogenase Reaction ◽  
Kinetics Of

Glutamate dehydrogenase and aspartate aminotransferase in leukocytes of patients with motor neuron disease

Neurology ◽  
1989 ◽  
Vol 39 (7) ◽  
pp. 956-956 ◽  
Author(s):  
J. Hugon ◽  
F. Tabaraud ◽  
M. Rigaud ◽  
J. M. Vallat ◽  
M. Dumas
Keyword(s):  
Motor Neuron ◽  
Motor Neuron Disease ◽  
Glutamate Dehydrogenase ◽  
Aspartate Aminotransferase
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